Identificação de genes de resistência a nematoides em alface (Lactuca sativa) por marcador molecular AFLP

• Autor(a) principal: Paulino, Willian Elias
• Data de Publicação: 2018
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFU
• Texto Completo: https://repositorio.ufu.br/handle/123456789/25541
• topic: Olericultura; Nematoide-das-galhas; Mapa de ligação; Vegetables crop; Root-knot nematode; Linkage map; CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL; CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS

The lettuce (Lactuca sativa) crop can be very affected by environmental factors, abiotic and biotic, like nematodes. In Brazil, most of the nematodes which are harmful to lettuce belong to genus Meloidogyne, which are installed in the root system of the plant forming root-knots. An alternative for the control of these nematodes is the use of resistant cultivars, but these aren’t usually pleasing to the market. Then, a cross was made between a resistant cultivar (Salinas 88) and a susceptible cultivar but that had market characteristics (Colorado), advancing another generation with self-fertilization. The samples used here were planted and evaluated at the Federal University of Lavras (UFLA). Using molecular marker AFLP, we sought to identify polymorphic marks linked to the resistance phenotype, making it possible to use this marker in breeding programs. With the formed marks, it was possible to determine a QTL for the resistance character.