PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2

Detalhes bibliográficos
Autor(a) principal: Souza, Mariana Tainná Silva
Data de Publicação: 2020
Outros Autores: Marinho, Barbhara Mota, Pasin, Thiago Machado, Nelson, David Lee, Benassi, Vivian Machado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista de Engenharia Química e Química
Texto Completo: https://periodicos.ufv.br/jcec/article/view/10560
Resumo: Amylases are used in numerous industrial applications for converting starch into products of greater value.  This work aimed to prospect filamentous fungi, analyze the morphological and physiological characteristics of the isolates; as well as to select an amylase producing fungus and to optimize the parameters for the cultivation of the microorganism and biochemically characterize the amylase. Among 21 filamentous fungi isolated in Janaúba, state of Minas Gerais, Brazil, the best amylase producer was selected for standardization of culture parameters and subsequent enzymatic characterization. Maximum activity was obtained in CP medium after six days of cultivation at 30 °C. Amylases produced by this fungus are stable to variations in pH and temperature, exhibited optimum activities at 65 oC and pH 6.0, and were significantly activated in the presence of 5 and 10 mm KH2PO4.
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spelling PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2EnzimasFungos FilamentososBioprospecçãoBiotecnologiaEnzymesFilamentous fungiBioprospectingBiotechnologyAmylases are used in numerous industrial applications for converting starch into products of greater value.  This work aimed to prospect filamentous fungi, analyze the morphological and physiological characteristics of the isolates; as well as to select an amylase producing fungus and to optimize the parameters for the cultivation of the microorganism and biochemically characterize the amylase. Among 21 filamentous fungi isolated in Janaúba, state of Minas Gerais, Brazil, the best amylase producer was selected for standardization of culture parameters and subsequent enzymatic characterization. Maximum activity was obtained in CP medium after six days of cultivation at 30 °C. Amylases produced by this fungus are stable to variations in pH and temperature, exhibited optimum activities at 65 oC and pH 6.0, and were significantly activated in the presence of 5 and 10 mm KH2PO4. As amilases são utilizadas em inúmeras aplicações industriais para a conversão de amido em produtos de maior valor agregado. Esse trabalho objetivou prospectar fungos filamentosos, analisar as características morfológicas e fisiológicas dos isolados, assim como, selecionar fungo produtor de amilases e otimizar os parâmetros de cultivo do microrganismo e caracterizar bioquimicamente a amilase. Dos vinte e um fungos filamentosos isolados em Janaúba, Minas Gerais, Brasil, foi selecionado o melhor produtor de amilases para padronização dos parâmetros de cultivo e posterior caracterização enzimática. A máxima atividade foi obtida em meio CP, após seis dias de cultivo, a 30 °C. As amilases produzidas são estáveis ao pH e à temperatura; apresentaram maiores atividades a 65 °C em pH 6,0 sendo significativamente ativadas na presença de 5 e 10 mM de KH2PO4. Universidade Federal de Viçosa - UFV2020-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufv.br/jcec/article/view/1056010.18540/jcecvl6iss3pp0365-0376The Journal of Engineering and Exact Sciences; Vol. 6 No. 3 (2020); 0365-0376The Journal of Engineering and Exact Sciences; Vol. 6 Núm. 3 (2020); 0365-0376The Journal of Engineering and Exact Sciences; v. 6 n. 3 (2020); 0365-03762527-1075reponame:Revista de Engenharia Química e Químicainstname:Universidade Federal de Viçosa (UFV)instacron:UFVenghttps://periodicos.ufv.br/jcec/article/view/10560/5918Copyright (c) 2020 The Journal of Engineering and Exact Sciencesinfo:eu-repo/semantics/openAccessSouza, Mariana Tainná Silva Marinho, Barbhara Mota Pasin, Thiago Machado Nelson, David LeeBenassi, Vivian Machado2021-02-23T20:12:27Zoai:ojs.periodicos.ufv.br:article/10560Revistahttp://www.seer.ufv.br/seer/rbeq2/index.php/req2/indexONGhttps://periodicos.ufv.br/jcec/oaijcec.journal@ufv.br||req2@ufv.br2446-94162446-9416opendoar:2021-02-23T20:12:27Revista de Engenharia Química e Química - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
title PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
spellingShingle PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
Souza, Mariana Tainná Silva
Enzimas
Fungos Filamentosos
Bioprospecção
Biotecnologia
Enzymes
Filamentous fungi
Bioprospecting
Biotechnology
title_short PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
title_full PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
title_fullStr PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
title_full_unstemmed PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
title_sort PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2
author Souza, Mariana Tainná Silva
author_facet Souza, Mariana Tainná Silva
Marinho, Barbhara Mota
Pasin, Thiago Machado
Nelson, David Lee
Benassi, Vivian Machado
author_role author
author2 Marinho, Barbhara Mota
Pasin, Thiago Machado
Nelson, David Lee
Benassi, Vivian Machado
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Souza, Mariana Tainná Silva
Marinho, Barbhara Mota
Pasin, Thiago Machado
Nelson, David Lee
Benassi, Vivian Machado
dc.subject.por.fl_str_mv Enzimas
Fungos Filamentosos
Bioprospecção
Biotecnologia
Enzymes
Filamentous fungi
Bioprospecting
Biotechnology
topic Enzimas
Fungos Filamentosos
Bioprospecção
Biotecnologia
Enzymes
Filamentous fungi
Bioprospecting
Biotechnology
description Amylases are used in numerous industrial applications for converting starch into products of greater value.  This work aimed to prospect filamentous fungi, analyze the morphological and physiological characteristics of the isolates; as well as to select an amylase producing fungus and to optimize the parameters for the cultivation of the microorganism and biochemically characterize the amylase. Among 21 filamentous fungi isolated in Janaúba, state of Minas Gerais, Brazil, the best amylase producer was selected for standardization of culture parameters and subsequent enzymatic characterization. Maximum activity was obtained in CP medium after six days of cultivation at 30 °C. Amylases produced by this fungus are stable to variations in pH and temperature, exhibited optimum activities at 65 oC and pH 6.0, and were significantly activated in the presence of 5 and 10 mm KH2PO4.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufv.br/jcec/article/view/10560
10.18540/jcecvl6iss3pp0365-0376
url https://periodicos.ufv.br/jcec/article/view/10560
identifier_str_mv 10.18540/jcecvl6iss3pp0365-0376
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.ufv.br/jcec/article/view/10560/5918
dc.rights.driver.fl_str_mv Copyright (c) 2020 The Journal of Engineering and Exact Sciences
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 The Journal of Engineering and Exact Sciences
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa - UFV
publisher.none.fl_str_mv Universidade Federal de Viçosa - UFV
dc.source.none.fl_str_mv The Journal of Engineering and Exact Sciences; Vol. 6 No. 3 (2020); 0365-0376
The Journal of Engineering and Exact Sciences; Vol. 6 Núm. 3 (2020); 0365-0376
The Journal of Engineering and Exact Sciences; v. 6 n. 3 (2020); 0365-0376
2527-1075
reponame:Revista de Engenharia Química e Química
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str Revista de Engenharia Química e Química
collection Revista de Engenharia Química e Química
repository.name.fl_str_mv Revista de Engenharia Química e Química - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv jcec.journal@ufv.br||req2@ufv.br
_version_ 1800211189403222016

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