Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)

Detalhes bibliográficos
Autor(a) principal: Faria, Filipe de Castro
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFVJM
Texto Completo: http://acervo.ufvjm.edu.br/jspui/handle/1/2029
Resumo: Ag?ncia financiadora: Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais ? (FAPEMIG) (CDS-APQ-02459-15).
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spelling Faria, Filipe de CastroGrael, Cristiane Fernanda FuzerSantos, Antonio SousaRuela, Fernando ArminiUniversidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Grael, Cristiane Fernanda Fuzer2019-08-15T18:22:23Z2019-08-15T18:22:23Z20192019-03-20FARIA, Filipe de Castro. Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae). 2019. 88 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2019.http://acervo.ufvjm.edu.br/jspui/handle/1/2029Ag?ncia financiadora: Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais ? (FAPEMIG) (CDS-APQ-02459-15).Ag?ncia financiadora (Bolsa de estudos): Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES).?rea de concentra??o: Ci?ncias Farmac?uticas.A utiliza??o de plantas medicinais regionais pela popula??o tem levado a um aumento dos estudos para analisar o potencial t?xico das plantas. Al?m disso, o interesse das ind?strias e institutos de pesquisa por plantas medicinais tamb?m aumentou. A esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae) ? conhecida popularmente como Matapasto ou Enxota e, ocorre naturalmente na regi?o de Cerrado, inclusive no munic?pio de Diamantina (MG), regi?o do Vale do Jequitinhonha e Mucuri. Essa planta ? utiliza com anti-inflamat?rio, analg?sico e cicatrizante usado topicamente em prepara??es alco?licas ou hidroalco?licas. Assim se faz necess?rio a triagem toxicol?gica da esp?cie bot?nica. A planta foi colhida no Campus JK da UFVJM e foram preparados o extrato etan?lico bruto das partes a?reas e suas fases hex?nica, dicloromet?nica e hidroalco?lica atrav?s de parti??o liquido-liquido. Foi realizado um screening fitoqu?mico para a identifica??o das principais classes de metab?litos especiais nas fases. Na fase hex?nica foram detectados cumarinas e triterpenos/esteroides; na fase dicloromet?nica foram detectados cumarinas, triterpenos/esteroides e taninos hidrolis?veis, na fase hidroalco?lica foram detectados alcaloides, cumarinas, saponinas, triterpenos/esteroides e taninos hidrolis?veis. Foi feita a an?lise das fases hex?nica e dicloromet?nica em CG/EM e foram identificados sesquiterpenos, constituintes graxos, triterpenos, base nitrogenada, monossacar?deo, alcaloide pirrolizid?nico (licopsamina), alde?do,cetona, compostos fen?licos. Foi utilizada CLAE/DAD para an?lise do perfil fitoqu?mico da fase hidroalco?lica, observando-se a presen?a de prov?vel de constituintes poliinsaturados. Para a triagem toxicol?gica do extrato bruto e de suas fases foram empregadas duas metodologias: ensaio de letalidade com o microcrust?ceo Artemia salina Leach, e o ensaio de viabilidade de c?lulas da linhagem L929, proveniente de tecido conjuntivo de camundongo. Os resultados demonstraram certa toxicidade do extrato e das fases, utilizando a artemia a fase hidroalco?lica apresentou maior toxicidade apresentando CL50 de 1,333mg/mL e o extrato etan?lico bruto obteve CL50 de 4,814mg/mL, sendo o menos t?xico. No ensaio com as c?lulas L929 a fase dicloromet?nica foi a mais t?xica, chegando a reduzir 95,48% da viabilidade das c?lulas e apresentando CC50 de 11,39 ?g/mL e a fase hidroalco?lica foi considerada a menos t?xica apresentando porcentagem de morte celular variando de 55,67 a 19,38, com CC50 de 174,2 ?g/mL. O estudo sugere que o extrato etan?lico de A. fastigiatum possui constitituintes que corroboram seu uso na medicina popular como anti-inflamat?rio e a detec??o de toxicidade pode ser um indicativo para avaliar a??o antitumoral do extrato e de subst?ncias isoladas da planta e para utilizar a planta na medicina popular de forma racional.Submitted by Nivaldo Melo (nivaldo.melo@ufvjm.edu.br) on 2019-08-14T17:58:52Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) filipe_castro_faria.pdf: 1879153 bytes, checksum: 792eae6480e8ea309f3d9e71259ec586 (MD5)Approved for entry into archive by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2019-08-15T18:22:23Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) filipe_castro_faria.pdf: 1879153 bytes, checksum: 792eae6480e8ea309f3d9e71259ec586 (MD5)Made available in DSpace on 2019-08-15T18:22:23Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) filipe_castro_faria.pdf: 1879153 bytes, checksum: 792eae6480e8ea309f3d9e71259ec586 (MD5) Previous issue date: 2019Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG)Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2019.The use of regional medicinal plants by the population has led to an increase in studies to analyze the toxic potential of plants. In addition, the interest of industries and research institutes for medicinal plants has also increased. The species Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae) is popularly known as Matapasto or Enxota and occurs naturally in the Cerrado region, including in the municipality of Diamantina (MG), Jequitinhonha Valley and Mucuri region. This plant is used with anti-inflammatory, analgesic and healing used topically in alcoholic or hydroalcoholic preparations. Thus, it is necessary the toxicological screening of the botanical species. The plant was harvested at Campus JK of the UFVJM and the crude ethanolic extract of the aerial parts and their hexane, dichloromethane and hydroalcoholic phases were prepared through a liquid-liquid partition. A phytochemical screening was carried out to identify the main classes of special metabolites in the phases. In the hexane phase coumarins and triterpenes / steroids were detected; in the dichloromethane phase, coumarins, triterpenes / steroids and hydrolysable tannins were detected. In the hydroalcoholic phase, alkaloids, coumarins, saponins, triterpenes / steroids and hydrolysable tannins were detected. The hexane and dichloromethane phases were analyzed in GC / MS and sesquiterpenes, fatty constituents, triterpenes, nitrogenous base, monosaccharide, pyrrolizidine alkaloid (licopsamine), aldehyde, ketone and phenolic compounds were identified. It was used CLAE / DAD to analyze the phytochemical profile of the hydroalcoholic phase, observing the presence of probable polyunsaturated constituents. For the toxicological screening of the crude extract and its phases two methodologies were used: lethality test with the microcrack Artemia salina Leach, and the viability assay of L929 lineage cells from mouse connective tissue. The results showed a certain toxicity of the extract and the phases, using the artemia the hydroalcoholic phase presented higher toxicity presenting LC50 of 1,333mg / mL and the raw ethanolic extract obtained LC50 of 4.814mg / mL, being the least toxic. In the L929 cell assay the dichloromethane phase was the most toxic, reducing 95.48% of cell viability and presenting CC50 of 11.39 ?g / mL and the hydroalcoholic phase was considered to be less toxic, with a percentage of cell death varying from 55.67 to 19.38, with CC50 of 174.2 ?g / mL. The study suggests that the ethanolic extract of A. fastigiatum has constituents that corroborate its use in folk medicine as an anti-inflammatory and the detection of toxicity can be an indicative to evaluate antitumor action of the extract and substances isolated from the plant and to use the plant in rational medicine.porUFVJMA concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data.info:eu-repo/semantics/openAccessEstudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. 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dc.title.pt_BR.fl_str_mv Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)
title Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)
spellingShingle Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)
Faria, Filipe de Castro
Plantas medicinais
Artemia salina
Citotoxicidade
Qu?mica de produto natural
Medicinal plants
Cytotoxicity
Natural product chemistry
title_short Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)
title_full Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)
title_fullStr Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)
title_full_unstemmed Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)
title_sort Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae)
author Faria, Filipe de Castro
author_facet Faria, Filipe de Castro
author_role author
dc.contributor.references.none.fl_str_mv Grael, Cristiane Fernanda Fuzer
Santos, Antonio Sousa
Ruela, Fernando Armini
dc.contributor.institution.pt_BR.fl_str_mv Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
dc.contributor.author.fl_str_mv Faria, Filipe de Castro
dc.contributor.advisor1.fl_str_mv Grael, Cristiane Fernanda Fuzer
contributor_str_mv Grael, Cristiane Fernanda Fuzer
dc.subject.keyword.pt_BR.fl_str_mv Plantas medicinais
Artemia salina
Citotoxicidade
Qu?mica de produto natural
topic Plantas medicinais
Artemia salina
Citotoxicidade
Qu?mica de produto natural
Medicinal plants
Cytotoxicity
Natural product chemistry
dc.subject.keyword.en.fl_str_mv Medicinal plants
Cytotoxicity
Natural product chemistry
description Ag?ncia financiadora: Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais ? (FAPEMIG) (CDS-APQ-02459-15).
publishDate 2019
dc.date.submitted.none.fl_str_mv 2019-03-20
dc.date.accessioned.fl_str_mv 2019-08-15T18:22:23Z
dc.date.available.fl_str_mv 2019-08-15T18:22:23Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIA, Filipe de Castro. Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae). 2019. 88 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2019.
dc.identifier.uri.fl_str_mv http://acervo.ufvjm.edu.br/jspui/handle/1/2029
identifier_str_mv FARIA, Filipe de Castro. Estudo fitoqu?mico e avalia??o de potencial t?xico da esp?cie Ageratum fastigiatum (Gardner) RM King & H. Rob (Asteraceae). 2019. 88 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2019.
url http://acervo.ufvjm.edu.br/jspui/handle/1/2029
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv UFVJM
publisher.none.fl_str_mv UFVJM
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFVJM
instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron:UFVJM
instname_str Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron_str UFVJM
institution UFVJM
reponame_str Repositório Institucional da UFVJM
collection Repositório Institucional da UFVJM
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