Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia

Detalhes bibliográficos
Autor(a) principal: Abreu, Caique Menezes de
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFVJM
Texto Completo: http://acervo.ufvjm.edu.br/jspui/handle/1/2107
Resumo: ?rea de concentra??o: Produ??o Vegetal.
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spelling Abreu, Caique Menezes deCosta, M?rcia Regina daMarriel, Ivanildo Ev?dioGrazziotti, Paulo HenriqueUniversidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Costa, M?rcia Regina da2019-11-29T21:09:44Z2019-11-29T21:09:44Z20192019-02-21ABREU, Caique Menezes de. Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia. 2019. 93 p. Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2019.http://acervo.ufvjm.edu.br/jspui/handle/1/2107?rea de concentra??o: Produ??o Vegetal.O presente trabalho foi realizado com apoio da Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? Brasil (CAPES) ? C?digo de Financiamento 001.As bact?rias diazotr?ficas podem aumentar a indu??o sist?mica de resist?ncia a pragas e doen?as, toler?ncia a condi??es adversas e fornecer fitohorm?nios e nutrientes para plantas de interesse florestal, mas essa tecnologia ainda n?o ? explorada. O objetivo do presente trabalho foi quantificar, isolar e caracterizar a popula??o de bact?rias diazotr?ficas endof?ticas e rizosf?rica em Eucalyptus e Corymbia e verificar a resposta de sensibilidade em plantas n?o alvo. A popula??o de bact?rias diazotr?ficas de folhas, ra?zes e rizosfera em Eucalyptus cloeziana, h?brido Eucalyptus urophylla x Eucalyptus grandis (urograndis) e Corymbia citriodora foi quantificada pelo m?todo do n?mero mais prov?vel, cultivadas em meio semis?lido livre de nitrog?nio. A partir dos cultivos semi-seletivos para quantifica??o de diazotr?fico foram isoladas 87 bact?rias. Estas estirpes foram caracterizadas morfologicamente por tipo de c?lulas e de col?nias; bioquimicamente pela produ??o de enzimas amilase, catalase, esterase-lipase, oxidase, urease e nitrato redutase e pela produ??o de ?cido indol-3-ac?tico (AIA), bacteriocinas, sider?foro e solubilizadores de fosfatos. A caracteriza??o gen?tica dos isolados foi estimada pela dist?ncia de dissimilaridade, por meio dos descritores morfol?gicos e bioqu?micos. A intera??o entre diazotr?ficos-plantas sem causar sintomas de doen?a foi verificada pelo m?todo da rea??o de hipersensibilidade induzida em plantas n?o alvo Capsicum annuum, Nicotiana tabacum e Solanum lycopersicum. Uma cole??o de bact?rias com potenciais promotores de crescimento vegetal foi estabelecida de maneira a preservar linhagens para investiga??es posteriores sobre seu potencial como inoculante para o setor florestal. Todos as 87 estirpes bacterianas al?m de fixarem N apresentaram a capacidade de produzir pelo menos mais um dos produtos investigados. A frequ?ncia das m?ltiplas fun??es foi de 92% para a catalase, 57,5% para fosfatase, 67% para urease, 69% para nitrato redutase, 40% para esteraselipase, 24% produ??o de sider?foro e 83% produ??o de ?cido indol-3-ac?tico. A diversidade microbiana gerou 38 grupos bacterianos, de Eucalyptus e Corymbia e microambientes diferentes, de interesse biotecnol?gico agron?mico e industrial. A atividade microbiana produtora de AIA dependente de L-triptofano foi observada em Herbaspirillum spp., Burkholderia spp., e Azospirillum spp. naturais dos tr?s microambientes da planta hospedeira, com produ??o de at? 411,3 ?g mL-1, escolhendo 28 estirpes. O ?ndice de solubilidade de P por bact?rias diazotr?ficas na forma de P-org?nico e P-inorg?nico selecionou de 38 estirpes com moderada a alta capacidade de solubiliza??o. As estirpes CctRZlgi-124, EhiFjnfb-34 e EhiFjnfb-36 podem ser utilizadas no controle biol?gico, com a??o antagonista a cepas de Xanthomonas spp. Todas as estirpes apresentaram resposta negativa a rea??o de hipersensibilidade induzida, significando potenciais agentes endof?ticos e rizosf?ricos ben?ficos as plantas de eucalipto. A densidade diazotr?fica em plantas de Corymbia citriodora, h?brido urograndis e Eucalyptus cloeziana diminui na medida que h? o afastamento do microambiente rizosf?rico para microambientes da filosfera, como ra?zes e folhas. A modifica??o da densidade de c?lulas bacterianas diazotr?ficas ? dada em fun??o do gen?tipo da planta hospedeira e ao microambiente. O clone Corymbia citriodora apresentou densidade maior que em rela??o ao h?brido urograndis e ao E. cloeziana. A multifuncionalidade de diazotr?ficos possibilita a elabora??o de arranjos e formula??es de inoculante espec?ficos e mistos para uso no setor florestal e ou industrial.Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2019-11-29T21:07:19Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) caique_menezes_abreu.pdf: 1638850 bytes, checksum: e63362938bce229946f654d2ef8c8976 (MD5)Approved for entry into archive by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2019-11-29T21:09:44Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) caique_menezes_abreu.pdf: 1638850 bytes, checksum: e63362938bce229946f654d2ef8c8976 (MD5)Made available in DSpace on 2019-11-29T21:09:44Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) caique_menezes_abreu.pdf: 1638850 bytes, checksum: e63362938bce229946f654d2ef8c8976 (MD5) Previous issue date: 2019Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2019.Diazotrophic bacteria can increase systemic induction of pest and disease resistance, tolerance to adverse conditions and provide phytohormones and nutrients for plants of forest interest, but this technology is not yet explored. The objective of the present work was to quantify, isolate and characterize the population of endophytic and rhizospheric diazotrophic bacteria in Eucalyptus and Corymbia and to verify the sensitivity response in non-target plants. The population of diazotrophic leaf, root and rhizosphere bacteria in Eucalyptus cloeziana, Eucalyptus urophylla x Eucalyptus grandis hybrids (urograndis) and Corymbia citriodora were quantified by the most probable number method, grown in nitrogen - free semi - solid medium. From the semi-selective cultures for diazotrophic quantification, 87 bacteria were isolated. These strains were characterized morphologically by cell type and colony; biochemically by the production of amylase, catalase, esterase-lipase, oxidase, urease and nitrate reductase enzymes and by the production of indole-3-acetic acid (AIA), bacteriocins, siderophore and phosphate solubilizers. The genetic characterization of the isolates was estimated by the distance of dissimilarity, through the morphological and biochemical descriptors. The interaction between diazotrophic plants without causing disease symptoms was verified by the induced hypersensitivity reaction method in non-target plants Capsicum annuum, Nicotiana tabacum and Solanum lycopersicum. A collection of bacteria with potential plant growth promoters has been established in order to preserve lineages for further investigation of their potential as an inoculant for the forest sector. All bacterial strains besides fixing N had the capacity to produce at least one more of the investigated products. The frequency of the multiple functions was 92% for catalase, 57.5% for phosphatase, 67% for urease, 69% for nitrate reductase, 40% for esterase-lipase, 24% siderophore production, and 83% indole acid production -3-acetic acid. The microbial diversity generated 38 bacterial groups, Eucalyptus and Corymbia and different microenvironments, of agronomic and industrial biotechnological interest. L-tryptophan-dependent AIA producing microbial activity was observed in Herbaspirillum spp., Burkholderia spp. and Azospirillum spp. of the three microenvironments of the host plant, yielding up to 411.3 ?g mL-1, choosing 28 strains. The solubility index of P by diazotrophic bacteria in the form of P-organic and P-inorganic selected from 38 strains with moderate to high solubilization capacity. The strains CctRZlgi-124, EhiFjnfb-34 and EhiFjnfb-36 can be used in biological control, with antagonistic action to strains of Xanthomonas spp. All strains showed a negative response to the induced hypersensitivity reaction, signifying potential endophytic and rhizospheric agents beneficial to eucalyptus plants. The diazotrophic density in plants of Corymbia citriodora, urograndis hybrid and Eucalyptus cloeziana decreases as there is a move away from the rhizospheric microenvironment to microenvironments of the phyllosphere, such as roots and leaves. The density modification of diazotrophic bacterial cells is given as a function of the genotype of the host plant and the microenvironment. The Corymbia citriodora clone presented higher density than that of the urograndis hybrid and E. cloeziana. The multifunctionality of diazotrophs enables the elaboration of specific and mixed inoculant formulations and arrangements for use in the forestry and or industrial sector.porUFVJMA concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data.info:eu-repo/semantics/openAccessQuantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e CorymbiaQuantification, isolation and characterization of diazotrophic bacteria in Eucalyptus and Corymbiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisDensidade microbianaDiazotr?ficoBiotecnologiaSolubilizadoresSider?foroMicrobial densityDiazotrophicBiotechnologySolubilizersSiderophorereponame:Repositório Institucional da UFVJMinstname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)instacron:UFVJMTEXTcaique_menezes_abreu.pdf.txtcaique_menezes_abreu.pdf.txtExtracted texttext/plain223770http://acervo.ufvjm.edu.br/jspui/bitstream/1/2107/6/caique_menezes_abreu.pdf.txt9e3282a28368973e3b808b821f7dc394MD56LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia
dc.title.alternative.en.fl_str_mv Quantification, isolation and characterization of diazotrophic bacteria in Eucalyptus and Corymbia
title Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia
spellingShingle Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia
Abreu, Caique Menezes de
Densidade microbiana
Diazotr?fico
Biotecnologia
Solubilizadores
Sider?foro
Microbial density
Diazotrophic
Biotechnology
Solubilizers
Siderophore
title_short Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia
title_full Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia
title_fullStr Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia
title_full_unstemmed Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia
title_sort Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia
author Abreu, Caique Menezes de
author_facet Abreu, Caique Menezes de
author_role author
dc.contributor.referee.none.fl_str_mv Costa, M?rcia Regina da
Marriel, Ivanildo Ev?dio
Grazziotti, Paulo Henrique
dc.contributor.institution.pt_BR.fl_str_mv Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
dc.contributor.author.fl_str_mv Abreu, Caique Menezes de
dc.contributor.advisor1.fl_str_mv Costa, M?rcia Regina da
contributor_str_mv Costa, M?rcia Regina da
dc.subject.keyword.pt_BR.fl_str_mv Densidade microbiana
Diazotr?fico
Biotecnologia
Solubilizadores
Sider?foro
topic Densidade microbiana
Diazotr?fico
Biotecnologia
Solubilizadores
Sider?foro
Microbial density
Diazotrophic
Biotechnology
Solubilizers
Siderophore
dc.subject.keyword.en.fl_str_mv Microbial density
Diazotrophic
Biotechnology
Solubilizers
Siderophore
description ?rea de concentra??o: Produ??o Vegetal.
publishDate 2019
dc.date.submitted.none.fl_str_mv 2019-02-21
dc.date.accessioned.fl_str_mv 2019-11-29T21:09:44Z
dc.date.available.fl_str_mv 2019-11-29T21:09:44Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv ABREU, Caique Menezes de. Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia. 2019. 93 p. Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2019.
dc.identifier.uri.fl_str_mv http://acervo.ufvjm.edu.br/jspui/handle/1/2107
identifier_str_mv ABREU, Caique Menezes de. Quantifica??o, isolamento e caracteriza??o de bact?rias diazotr?ficas em Eucalyptus e Corymbia. 2019. 93 p. Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2019.
url http://acervo.ufvjm.edu.br/jspui/handle/1/2107
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv UFVJM
publisher.none.fl_str_mv UFVJM
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFVJM
instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron:UFVJM
instname_str Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron_str UFVJM
institution UFVJM
reponame_str Repositório Institucional da UFVJM
collection Repositório Institucional da UFVJM
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