Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis

Detalhes bibliográficos
Autor(a) principal: Spencer, Paula Villela Dessimoni
Data de Publicação: 2022
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFVJM
Texto Completo: http://acervo.ufvjm.edu.br/jspui/handle/1/3139
Resumo: O orientador do trabalho n?o mencionado na lista da Folha de Aprova??o.
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spelling Spencer, Paula Villela DessimoniNelson, David LeeSantos, Sandro Luiz Barbosa dosMeira Pires, Juliana Rocha deAra?jo, Clin?scia Rodrigues RochaLucas, Rosymar Coutinho deFid?ncio, Paulo HenriqueBenassi, Vivian MachadoUniversidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Nelson, David Lee2023-03-09T21:17:04Z2023-03-09T21:17:04Z20222022-10-18SPENCER, Paula Villela Dessimoni. Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis. 2022. 114 p. Tese (Doutorado em Biocombust?veis) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2022.http://acervo.ufvjm.edu.br/jspui/handle/1/3139O orientador do trabalho n?o mencionado na lista da Folha de Aprova??o.Muitos microrganismos secretam enzimas durante o seu crescimento, uma dessas enzimas s?o as lipases que s?o utilizadas em v?rios setores biotecnol?gicos. Uma de suas aplica??es ? na rea??o de transesterifica??o para produ??o de biodiesel. O objetivo foi coletar, isolar fungos filamentosos produtores de lipase e analisar as caracter?sticas morfol?gicas macrosc?picas dos fungos filamentosos isolados e do banco de fungos do laborat?rio, assim como, padronizar o cultivo do microrganismo selecionado para uma maior atividade lipol?tica, seguindo caracteriza??o bioqu?mica da lipase sol?vel produzida em meio submerso e caracteriza??o f?sico-qu?mica dos ?leos vegetais. Sendo assim foi poss?vel isolar doze fungos filamentosos de distintas amostras coletadas em Diamantina-MG, cuja maioria apresentou colora??o branca, aspecto seco, topografia plana e pigmenta??o. Analisou-se o efeito da temperatura no crescimento dos doze fungos isolados e dos oito fungos filamentosos do banco de fungo do laborat?rio em meio de cultura s?lido contendo aveia e Batata-Dextrose-?gar (BDA), onde obteve-se as maiores taxas de crescimento em ambos os meios de cultura a 30?C. Pela triagem de produ??o lipol?tica o fungo do banco 3.2TA pertencente ao filo Mucoromycota e obteve o maior halo de crescimento, sendo selecionado para a fermenta??o submersa. Analisaram-se cinco meios de cultura submersos para a produ??o de lipase e a melhor atividade foi obtida no meio Adams ap?s 72 horas de cultivo, seguindo a otimiza??o, observou-se que a maior atividade na fonte de nitrog?nio foi em ureia, peptona e extrato de levedura. Para a fontes de sais a maior atividade foi em sais CP e Khanna com pH inicial de 4,0. A fonte de carbono foi analisada e a melhor atividade foi obtida em ?leo de oliva. Foi realizada a caracteriza??o bioqu?mica da enzima sobre os efeitos do pH e temperatura onde apresentou melhor atividade nas faixas de pHs 7-14 a altas temperaturas. A termoestabilidade da enzima foi a 55oC nos tempos de 60 e 90 min. A estabilidade ao pH foi a um pH de 5,5, nos tempos de incuba??o de e 60 min. A estabilidade a solventes foi obtida em metanol e etanol. A maior hidr?lise enzim?tica ocorreu em azeite de oliva, seguido do ?leo de algod?o, soja e milho. Foi realizada a caracteriza??o f?sico qu?mica de diferentes ?leos vegetais, o maior ?ndice de acidez foi no ?leo de dend? e de oliva. O maior valor para ?ndice de peroxido foi em ?leo de abacate, oliva, pequi e os menores em ?leo de trigo. Para o ?ndice de saponifica??o os maiores valores foram no ?leo de abacate, trigo, canola. Para o material insaponific?vel os maiores valores foram nos ?leos de soja, algod?o, girassol, e o menor valor no ?leo de dend?. Sendo esses par?metros f?sicos-qu?micos importantes a serem realizados em mat?rias primas como ?leos vegetais que s?o utilizadas na produ??o de biodiesel.Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2023-03-09T21:10:47Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) paula_villela_dessimoni_spencer.pdf: 1781463 bytes, checksum: 04b20e0ea19378a2b699cbe908c48fe0 (MD5)Approved for entry into archive by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2023-03-09T21:17:04Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) paula_villela_dessimoni_spencer.pdf: 1781463 bytes, checksum: 04b20e0ea19378a2b699cbe908c48fe0 (MD5)Made available in DSpace on 2023-03-09T21:17:04Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) paula_villela_dessimoni_spencer.pdf: 1781463 bytes, checksum: 04b20e0ea19378a2b699cbe908c48fe0 (MD5) Previous issue date: 2022Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq)Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG)Tese (Doutorado) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2022.Many microorganisms secrete enzymes during their growth, one of these enzymes are lipases that are used in various biotechnological sectors. One of its applications is in the transesterification reaction for biodiesel production. The objective was to collect, isolate lipase-producing filamentous fungi and analyze the macroscopic morphological characteristics of the isolated filamentous fungi and the laboratory fungal bank, as well as standardize the cultivation of the selected microorganism for greater lipolytic activity, following biochemical characterization of the soluble lipase. produced in submerged medium and physicochemical characterization of vegetable oils. Thus, it was possible to isolate twelve filamentous fungi from different samples collected in Diamantina-MG, most of which presented white coloration, dry appearance, flat topography and pigmentation. The effect of temperature on the growth of the twelve isolated fungi and of the eight filamentous fungi from the fungus bank in the laboratory was analyzed in a solid culture medium containing oat and Potato-Dextrose-Agar (PDA), where the highest growth rates were obtained. in both culture media at 30?C. By screening for lipolytic production, the fungus from bank 3.2TA belonging to the phylum Mucoromycota obtained the highest growth halo, being selected for submerged fermentation. Five submerged culture media were analyzed for the production of lipase and the best activity was obtained in Adams medium after 72 hours of cultivation, following the optimization, it was observed that the highest activity in the nitrogen source was in urea, peptone and extract of yeast. For the salt sources, the highest activity was in CP and Khanna salts with an initial pH of 4.0. The carbon source was analyzed and the best activity was obtained in olive oil. The biochemical characterization of the enzyme was carried out on the effects of pH and temperature where it showed better activity in the ranges of pH 7-14 at high temperatures. The enzyme thermostability was at 55oC for 60 and 90 min. The pH stability was at a pH of 5.5, at incubation times of and 60 min. Solvent stability was achieved in methanol and ethanol. The highest enzymatic hydrolysis occurred in olive oil, followed by cotton, soybean and corn oil. The physicochemical characterization of different vegetable oils was carried out, the highest acidity index was in palm and olive oil. The highest value for peroxide index was in avocado, olive, pequi oil and the lowest in wheat oil. For the saponification index the highest values were in avocado, wheat and canola oil. For the unsaponifiable material, the highest values were in soybean, cotton, sunflower oils, and the lowest value in palm oil. These physical-chemical parameters are important to be performed in raw materials such as vegetable oils that are used in the production of biodiesel.porUFVJMA concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data.info:eu-repo/semantics/openAccessCaracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisFungos filamentososLipasesCaracteriza??o enzim?ticaBiodieselFilamentous fungiLipasesEnzymatic characterizationBiodieselreponame:Repositório Institucional da UFVJMinstname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)instacron:UFVJMTEXTpaula_villela_dessimoni_spencer.pdf.txtpaula_villela_dessimoni_spencer.pdf.txtExtracted texttext/plain274089http://acervo.ufvjm.edu.br/jspui/bitstream/1/3139/6/paula_villela_dessimoni_spencer.pdf.txta352739d388e11f6ad58b539fa5adde6MD56LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis
title Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis
spellingShingle Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis
Spencer, Paula Villela Dessimoni
Fungos filamentosos
Lipases
Caracteriza??o enzim?tica
Biodiesel
Filamentous fungi
Lipases
Enzymatic characterization
Biodiesel
title_short Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis
title_full Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis
title_fullStr Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis
title_full_unstemmed Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis
title_sort Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis
author Spencer, Paula Villela Dessimoni
author_facet Spencer, Paula Villela Dessimoni
author_role author
dc.contributor.referee.none.fl_str_mv Nelson, David Lee
Santos, Sandro Luiz Barbosa dos
Meira Pires, Juliana Rocha de
Ara?jo, Clin?scia Rodrigues Rocha
Lucas, Rosymar Coutinho de
Fid?ncio, Paulo Henrique
dc.contributor.advisorco.none.fl_str_mv Benassi, Vivian Machado
dc.contributor.institution.pt_BR.fl_str_mv Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
dc.contributor.author.fl_str_mv Spencer, Paula Villela Dessimoni
dc.contributor.advisor1.fl_str_mv Nelson, David Lee
contributor_str_mv Nelson, David Lee
dc.subject.keyword.pt_BR.fl_str_mv Fungos filamentosos
Lipases
Caracteriza??o enzim?tica
Biodiesel
topic Fungos filamentosos
Lipases
Caracteriza??o enzim?tica
Biodiesel
Filamentous fungi
Lipases
Enzymatic characterization
Biodiesel
dc.subject.keyword.en.fl_str_mv Filamentous fungi
Lipases
Enzymatic characterization
Biodiesel
description O orientador do trabalho n?o mencionado na lista da Folha de Aprova??o.
publishDate 2022
dc.date.submitted.none.fl_str_mv 2022-10-18
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-03-09T21:17:04Z
dc.date.available.fl_str_mv 2023-03-09T21:17:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SPENCER, Paula Villela Dessimoni. Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis. 2022. 114 p. Tese (Doutorado em Biocombust?veis) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2022.
dc.identifier.uri.fl_str_mv http://acervo.ufvjm.edu.br/jspui/handle/1/3139
identifier_str_mv SPENCER, Paula Villela Dessimoni. Caracteriza??o bioqu?mica de lipases de fungos filamentosos visando a produ??o de biocombust?veis. 2022. 114 p. Tese (Doutorado em Biocombust?veis) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2022.
url http://acervo.ufvjm.edu.br/jspui/handle/1/3139
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv UFVJM
publisher.none.fl_str_mv UFVJM
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFVJM
instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron:UFVJM
instname_str Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron_str UFVJM
institution UFVJM
reponame_str Repositório Institucional da UFVJM
collection Repositório Institucional da UFVJM
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