O desenvolvimento do aparelho reprodutor de machos diplóides de Melipona quadrifasciata Lepeletier, 1836 sob influência do hormônio juvenil

• Autor(a) principal: Ferreira, Ríudo de Paiva
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2343
• topic: Abelhas indígenas sem-ferrão; Machos diplóides; Hormônio juvenil; Indigenous stingless bees; Diploid males; Juvenile hormone; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Topical application of juvenile hormone (JH) in last instar larvae, during the cocoon spin of Melipona quadrifasciata larvae induces differentiation of workers larvae in Queens, the same effect was observed in diploid males of this species. Males that were morphologically similar to the queen were "Like Queen Male" (QLM). The objective of this study was to characterize the morphology of the testes, seminal vesicles and sperm haploid and diploid males of similar to the queen by the treatment with 2 μg / ml HJ in order to elucidate the effect of this hormone in the development of the characteristics of the reproductive structures of males of M. quadrifasciata. We made histological and ultrastructural analysis of the testes, seminal vesicles and sperm of 21 newly emerged QLM and the ages of three and five days of adulthood. The same structures of 21 haploid and 21 diploid males natural at the same ages were used for comparison. Haploid and diploid males, the QLM had lower survival time. In the QLM, we observed a rapid degeneration of internal organs. The testes of the QLM are smaller than those of haploid and diploid males. Spermatozoa of haploid and diploid males are very similar, in the QLM the sperm are not formed. The seminal vesicle of the QLM is also undergoing a rapid process of cell degeneration and portions are observed in the light organ in newly emerged males. From the data presented, the HJ seems to shorten the life span of males QLM.