Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum

Detalhes bibliográficos
Autor(a) principal: Carvalho, Izabela Maria Montezano de
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/2413
Resumo: Brazil possesses the most abundant and diverse plant life in the world, home to nearly 22% of all higher plant species on the planet. Traditional knowledge of many of these Brazilian plants is associated to this genetic patrimony, especially the use of plants in popular medicine. Therefore, the proposal of the present work was to evaluate the antihepatotoxic potential of extracts obtained of bark and leafs from plant species popularly used for this purpose, Strychnos pseudoquina A. St.- Hil., family: Loganiaceae and Coutarea hexandra (Jacq.) K. Schum, family: Rubiaceae. A preliminary phytochemical analysis was separately performed on the ethanol extract of bark and leafs by thin film chromatography; as well as an in vitro evaluation of antioxidant potential. Subsequently, biological tests were conducted toinvestigate the capacity of the evaluated extracts to revert hepatic damage inducted by paracetamol in rats. In vivo antihepatotoxic tests were performed by employing three distinctive doses and thereafter, evaluating biochemical and histological parameters of the animals. From the preliminary phytochemical analysis, differences in the composition of evaluated secondary metabolitegroups were observed between the bark and leaf extracts for each of the studied species. In relation to antioxidant potential, in both species, the bark extracts showed better results compared with leafs. In the biological testof the species S. pseudoquina, the bark (100mg/kg) and leaf (200mg/kg) extracts presented possible beneficiary effects on the metabolism of the hepatobiliary system. On the other hand, results indicated the incapability of these extracts to revert the hepatocellular damages caused by the paracetamol. In the same test model conducted for C. hexandra, a weak antihepatotoxic effect was observed in toxicity induced by parecetamol from the bark extract (100mg/kg), while the leaf extract showed no activity. Histological observations confirmed the beneficiary effect of the bark extracts from both species against liver damage induced by paracetamol in rats. A possible acting mechanism may involve antioxidant activity of this extract, principally promoted by polyphenolic metabolites.
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spelling Carvalho, Izabela Maria Montezano dehttp://lattes.cnpq.br/0103210968160524Oliveira, Tânia Toledo dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787758J2Fonseca, Cláudio Césarhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780777E6Leite, João Paulo Vianahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763897U8Fontes, Elita Sciohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794299J7Diaz, Marisa Alves Nogueirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723344J72015-03-26T13:07:28Z2011-04-042015-03-26T13:07:28Z2009-07-14CARVALHO, Izabela Maria Montezano de. Evaluation of antihepatotoxic activity of two plant species popularly known as "quina": Strychnos pseudoquina A. St. Hil. and Coutarea hexandra (Jacq.) K. Schum. 2009. 94 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2009.http://locus.ufv.br/handle/123456789/2413Brazil possesses the most abundant and diverse plant life in the world, home to nearly 22% of all higher plant species on the planet. Traditional knowledge of many of these Brazilian plants is associated to this genetic patrimony, especially the use of plants in popular medicine. Therefore, the proposal of the present work was to evaluate the antihepatotoxic potential of extracts obtained of bark and leafs from plant species popularly used for this purpose, Strychnos pseudoquina A. St.- Hil., family: Loganiaceae and Coutarea hexandra (Jacq.) K. Schum, family: Rubiaceae. A preliminary phytochemical analysis was separately performed on the ethanol extract of bark and leafs by thin film chromatography; as well as an in vitro evaluation of antioxidant potential. Subsequently, biological tests were conducted toinvestigate the capacity of the evaluated extracts to revert hepatic damage inducted by paracetamol in rats. In vivo antihepatotoxic tests were performed by employing three distinctive doses and thereafter, evaluating biochemical and histological parameters of the animals. From the preliminary phytochemical analysis, differences in the composition of evaluated secondary metabolitegroups were observed between the bark and leaf extracts for each of the studied species. In relation to antioxidant potential, in both species, the bark extracts showed better results compared with leafs. In the biological testof the species S. pseudoquina, the bark (100mg/kg) and leaf (200mg/kg) extracts presented possible beneficiary effects on the metabolism of the hepatobiliary system. On the other hand, results indicated the incapability of these extracts to revert the hepatocellular damages caused by the paracetamol. In the same test model conducted for C. hexandra, a weak antihepatotoxic effect was observed in toxicity induced by parecetamol from the bark extract (100mg/kg), while the leaf extract showed no activity. Histological observations confirmed the beneficiary effect of the bark extracts from both species against liver damage induced by paracetamol in rats. A possible acting mechanism may involve antioxidant activity of this extract, principally promoted by polyphenolic metabolites.O Brasil possui a maior e mais diversa flora mundial, sendo detentor de cerca de 22% de todas as espécies de plantas superiores do planeta. Ao mesmo tempo, o conhecimento tradicional de várias culturas brasileiras está associado a esse patrimônio genético, sobretudo com o uso de plantas na medicina popular. Nesse contexto, o presente trabalho teve como propósito avaliar o potencial antihepatotóxico de extratos obtidos de cascas e folhas de duas espécies vegetais utilizadas popularmente com esta finalidade, as quinas Strychnos pseudoquina A. St.-Hil., família Loganiaceae, e Coutarea hexandra (Jacq.) K. Schum, família Rubiaceae. Foi realizada a prospecção fitoquímica preliminar, por cromatografia em camada delgada, dos extratos etanólicos de cascas e folhas, separadamente, destas espécies e avaliado o potencial antioxidante in vitro. Posteriormente, ensaios biológicos foram conduzidos para investigar a capacidade dos extratos avaliados em reverter injúria hepática induzida por paracetamol em ratos. Os testes in vivo antihepatotóxico foram realizados empregando-se três doses distintas, sendo ao final do experimento, avaliados parâmetros bioquímicos e histológicos dos animais. Pela prospecção fitoquímica preliminar observaram-se diferenças na composição dos grupos de metabólitos secundários avaliados entre os extratos de cascas e folhas para cada uma das espécies. Em relação ao potencial antioxidante, em ambas as espécies, os extratos provenientes das cascas demonstraram melhores resultados em relação aos da folha. No ensaio biológico da espécie S. pseudoquina, o extrato de cascas (100mg/kg) e de folhas (200mg/kg) apresentaram possível efeito benéfico no metabolismo do sistema hepatobiliar. Por outro lado, os resultados indicam a incapacidade desses extratos em reverter as lesões hepatocelulares causadas pelo paracetamol. No mesmo modelo de ensaio conduzido para a C. hexandra, observou-se fraco efeito antihepatotóxico em toxicidade induzida por paracetamol do extrato de cascas (100mg/kg), enquanto o extrato das folhas não mostrou atividade. Observações histológicas confirmam o efeito benéfico dos extratos de cascas de ambas as espécies contra injúrias do fígado induzidas por paracetamol em ratos. Possível mecanismo pode envolver a atividade antioxidante desse extrato, promovido principalmente por metabólitos polifenólicos.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Bioquímica AgrícolaUFVBRBioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animalQuinaAtividade antihepatotóxicaQuinaAntihepatotoxic activityCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAvaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. SchumEvaluation of antihepatotoxic activity of two plant species popularly known as "quina": Strychnos pseudoquina A. St. Hil. and Coutarea hexandra (Jacq.) K. 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dc.title.por.fl_str_mv Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum
dc.title.alternative.eng.fl_str_mv Evaluation of antihepatotoxic activity of two plant species popularly known as "quina": Strychnos pseudoquina A. St. Hil. and Coutarea hexandra (Jacq.) K. Schum
title Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum
spellingShingle Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum
Carvalho, Izabela Maria Montezano de
Quina
Atividade antihepatotóxica
Quina
Antihepatotoxic activity
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum
title_full Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum
title_fullStr Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum
title_full_unstemmed Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum
title_sort Avaliação de atividade antihepatotóxica de duas espécies vegetais popularmente conhecidas como "quina": Strychnos pseudoquina A. St. Hil. e Coutarea hexandra (Jacq.) K. Schum
author Carvalho, Izabela Maria Montezano de
author_facet Carvalho, Izabela Maria Montezano de
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/0103210968160524
dc.contributor.author.fl_str_mv Carvalho, Izabela Maria Montezano de
dc.contributor.advisor-co1.fl_str_mv Oliveira, Tânia Toledo de
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787758J2
dc.contributor.advisor-co2.fl_str_mv Fonseca, Cláudio César
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780777E6
dc.contributor.advisor1.fl_str_mv Leite, João Paulo Viana
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763897U8
dc.contributor.referee1.fl_str_mv Fontes, Elita Scio
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794299J7
dc.contributor.referee2.fl_str_mv Diaz, Marisa Alves Nogueira
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723344J7
contributor_str_mv Oliveira, Tânia Toledo de
Fonseca, Cláudio César
Leite, João Paulo Viana
Fontes, Elita Scio
Diaz, Marisa Alves Nogueira
dc.subject.por.fl_str_mv Quina
Atividade antihepatotóxica
topic Quina
Atividade antihepatotóxica
Quina
Antihepatotoxic activity
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
dc.subject.eng.fl_str_mv Quina
Antihepatotoxic activity
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Brazil possesses the most abundant and diverse plant life in the world, home to nearly 22% of all higher plant species on the planet. Traditional knowledge of many of these Brazilian plants is associated to this genetic patrimony, especially the use of plants in popular medicine. Therefore, the proposal of the present work was to evaluate the antihepatotoxic potential of extracts obtained of bark and leafs from plant species popularly used for this purpose, Strychnos pseudoquina A. St.- Hil., family: Loganiaceae and Coutarea hexandra (Jacq.) K. Schum, family: Rubiaceae. A preliminary phytochemical analysis was separately performed on the ethanol extract of bark and leafs by thin film chromatography; as well as an in vitro evaluation of antioxidant potential. Subsequently, biological tests were conducted toinvestigate the capacity of the evaluated extracts to revert hepatic damage inducted by paracetamol in rats. In vivo antihepatotoxic tests were performed by employing three distinctive doses and thereafter, evaluating biochemical and histological parameters of the animals. From the preliminary phytochemical analysis, differences in the composition of evaluated secondary metabolitegroups were observed between the bark and leaf extracts for each of the studied species. In relation to antioxidant potential, in both species, the bark extracts showed better results compared with leafs. In the biological testof the species S. pseudoquina, the bark (100mg/kg) and leaf (200mg/kg) extracts presented possible beneficiary effects on the metabolism of the hepatobiliary system. On the other hand, results indicated the incapability of these extracts to revert the hepatocellular damages caused by the paracetamol. In the same test model conducted for C. hexandra, a weak antihepatotoxic effect was observed in toxicity induced by parecetamol from the bark extract (100mg/kg), while the leaf extract showed no activity. Histological observations confirmed the beneficiary effect of the bark extracts from both species against liver damage induced by paracetamol in rats. A possible acting mechanism may involve antioxidant activity of this extract, principally promoted by polyphenolic metabolites.
publishDate 2009
dc.date.issued.fl_str_mv 2009-07-14
dc.date.available.fl_str_mv 2011-04-04
2015-03-26T13:07:28Z
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dc.identifier.citation.fl_str_mv CARVALHO, Izabela Maria Montezano de. Evaluation of antihepatotoxic activity of two plant species popularly known as "quina": Strychnos pseudoquina A. St. Hil. and Coutarea hexandra (Jacq.) K. Schum. 2009. 94 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2009.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/2413
identifier_str_mv CARVALHO, Izabela Maria Montezano de. Evaluation of antihepatotoxic activity of two plant species popularly known as "quina": Strychnos pseudoquina A. St. Hil. and Coutarea hexandra (Jacq.) K. Schum. 2009. 94 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2009.
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