Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus

Detalhes bibliográficos
Autor(a) principal: Galinari, Éder
Data de Publicação: 2017
Outros Autores: Sabry, Diego Araújo, Sassaki, Guilherme Lanzi, Macedo, Gorete Ribeiro, Passos, Flávia Maria Lopes, Mantovani, Hilário Cuquetto, Rocha, Hugo Alexandre Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.carbpol.2016.11.015
http://www.locus.ufv.br/handle/123456789/19014
Resumo: Cell wall polysaccharides from filamentous fungi and yeasts have been reported as antioxidant and antiproliferative polymers. Thus, we evaluated these activities from cell wall polysaccharides from Kluyveromyces marxianus CCT7735. By using a centrifugal filter, a 203 kDa α-d-mannan (KMM-5) was obtained. KMM-5 exhibited no effect on HeLa cells and a weak antiproliferative activity against Hep-G2 cells. In addition, at higher concentrations, it presented a cytotoxicity to the normal cell line, 3T3. However, KMM-5 showed copper- and iron-chelating abilities, the latter of which presented improved activity. By using 2D-NMR COSY, HSQC edited and HMBC experiments, a structure arrangement was proposed. The main chain was formed by 6)-α-d-Manp-(1 → 6) units substituted at the 2-O-position by non-reducing terminals α-d-Manp-(1 → 2) and by a branched tetrasaccharide. The latter was formed by an internal 2)-α-d-Manp-(1 → 2) unit with linked to it a 2,3)-α-d-Manp-(1 → 2) unit substituted at the 2-O-position by a non-reducing terminal α-d-Manp-(1 → 2), and at the 3-O-position by a non-reducing terminal α-d-Manp-(1 → 3). In conclusion, we considered K. marxianus CCT7735 a source of natural and renewable polysaccharides with pharmacological properties.
id UFV_03065447b1a179b5570cfe2d6d5a84ec
oai_identifier_str oai:locus.ufv.br:123456789/19014
network_acronym_str UFV
network_name_str LOCUS Repositório Institucional da UFV
repository_id_str 2145
spelling Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianusNMRPolysaccharideCopper chelatorIron chelatorTumor cellCell wall polysaccharides from filamentous fungi and yeasts have been reported as antioxidant and antiproliferative polymers. Thus, we evaluated these activities from cell wall polysaccharides from Kluyveromyces marxianus CCT7735. By using a centrifugal filter, a 203 kDa α-d-mannan (KMM-5) was obtained. KMM-5 exhibited no effect on HeLa cells and a weak antiproliferative activity against Hep-G2 cells. In addition, at higher concentrations, it presented a cytotoxicity to the normal cell line, 3T3. However, KMM-5 showed copper- and iron-chelating abilities, the latter of which presented improved activity. By using 2D-NMR COSY, HSQC edited and HMBC experiments, a structure arrangement was proposed. The main chain was formed by 6)-α-d-Manp-(1 → 6) units substituted at the 2-O-position by non-reducing terminals α-d-Manp-(1 → 2) and by a branched tetrasaccharide. The latter was formed by an internal 2)-α-d-Manp-(1 → 2) unit with linked to it a 2,3)-α-d-Manp-(1 → 2) unit substituted at the 2-O-position by a non-reducing terminal α-d-Manp-(1 → 2), and at the 3-O-position by a non-reducing terminal α-d-Manp-(1 → 3). In conclusion, we considered K. marxianus CCT7735 a source of natural and renewable polysaccharides with pharmacological properties.Carbohydrate Polymers2018-04-23T11:22:39Z2018-04-23T11:22:39Z2017-02-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf01448617https://doi.org/10.1016/j.carbpol.2016.11.015http://www.locus.ufv.br/handle/123456789/19014engv. 157, p. 1298-1305, feb. 2017Galinari, ÉderSabry, Diego AraújoSassaki, Guilherme LanziMacedo, Gorete RibeiroPassos, Flávia Maria LopesMantovani, Hilário CuquettoRocha, Hugo Alexandre Oliveirainfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:32:48Zoai:locus.ufv.br:123456789/19014Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:32:48LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus
title Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus
spellingShingle Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus
Galinari, Éder
NMR
Polysaccharide
Copper chelator
Iron chelator
Tumor cell
title_short Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus
title_full Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus
title_fullStr Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus
title_full_unstemmed Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus
title_sort Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus
author Galinari, Éder
author_facet Galinari, Éder
Sabry, Diego Araújo
Sassaki, Guilherme Lanzi
Macedo, Gorete Ribeiro
Passos, Flávia Maria Lopes
Mantovani, Hilário Cuquetto
Rocha, Hugo Alexandre Oliveira
author_role author
author2 Sabry, Diego Araújo
Sassaki, Guilherme Lanzi
Macedo, Gorete Ribeiro
Passos, Flávia Maria Lopes
Mantovani, Hilário Cuquetto
Rocha, Hugo Alexandre Oliveira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Galinari, Éder
Sabry, Diego Araújo
Sassaki, Guilherme Lanzi
Macedo, Gorete Ribeiro
Passos, Flávia Maria Lopes
Mantovani, Hilário Cuquetto
Rocha, Hugo Alexandre Oliveira
dc.subject.por.fl_str_mv NMR
Polysaccharide
Copper chelator
Iron chelator
Tumor cell
topic NMR
Polysaccharide
Copper chelator
Iron chelator
Tumor cell
description Cell wall polysaccharides from filamentous fungi and yeasts have been reported as antioxidant and antiproliferative polymers. Thus, we evaluated these activities from cell wall polysaccharides from Kluyveromyces marxianus CCT7735. By using a centrifugal filter, a 203 kDa α-d-mannan (KMM-5) was obtained. KMM-5 exhibited no effect on HeLa cells and a weak antiproliferative activity against Hep-G2 cells. In addition, at higher concentrations, it presented a cytotoxicity to the normal cell line, 3T3. However, KMM-5 showed copper- and iron-chelating abilities, the latter of which presented improved activity. By using 2D-NMR COSY, HSQC edited and HMBC experiments, a structure arrangement was proposed. The main chain was formed by 6)-α-d-Manp-(1 → 6) units substituted at the 2-O-position by non-reducing terminals α-d-Manp-(1 → 2) and by a branched tetrasaccharide. The latter was formed by an internal 2)-α-d-Manp-(1 → 2) unit with linked to it a 2,3)-α-d-Manp-(1 → 2) unit substituted at the 2-O-position by a non-reducing terminal α-d-Manp-(1 → 2), and at the 3-O-position by a non-reducing terminal α-d-Manp-(1 → 3). In conclusion, we considered K. marxianus CCT7735 a source of natural and renewable polysaccharides with pharmacological properties.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-10
2018-04-23T11:22:39Z
2018-04-23T11:22:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 01448617
https://doi.org/10.1016/j.carbpol.2016.11.015
http://www.locus.ufv.br/handle/123456789/19014
identifier_str_mv 01448617
url https://doi.org/10.1016/j.carbpol.2016.11.015
http://www.locus.ufv.br/handle/123456789/19014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 157, p. 1298-1305, feb. 2017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Carbohydrate Polymers
publisher.none.fl_str_mv Carbohydrate Polymers
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
_version_ 1817559938835152896