Biomarcadores inflamatórios e determinantes da síndrome metabólica em adultos jovens saudáveis

• Autor(a) principal: Rocha, José Luiz Marques
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2769
• topic: Biomarcadores inflamatórios; TNF-α IL-6; Síndrome metabólica; Inflammatory biomarkers; TNF-α IL-6; Metabolic syndrome; CNPQ::CIENCIAS DA SAUDE::NUTRICAO

Several chronic diseases are accompanied by inflammatory processes and evidence continues to accumulate on the clinical relevance of these laboratory predictors of pathophysiological events. In this context, the present study was to evaluate, in young and clinically healthy individuals, plasma concentrations of TNF- and IL-6 and the expression of genes related to inflammation (TNF-&#945;, IL-6, ICAM1, IL1R1 and IL-18) and their possible association with risk factors for the development of metabolic syndrome (MS). 161 individuals were evaluated about the anthropometry, body composition, dietary intake, and biochemical and clinical datas. The plasma level of TNF-&#945; and IL-6 was conducted by immunoassay (ELISA) in EDTA plasma. Gene expression (GE) was performed on periferial blood mononuclear cells (PBMC) using real time PCR. Comparisons between groups were performed by the Mann-Whitney-U or Student-t in accordance with the distribution of variables. We used Spearman correlation, the linear regression model and the logistic regression. The results were presented as mean ± SD, interval of 95% and significance level of P <0.05. The results showed that plasma concentrations of TNF-&#945; and IL-6 were higher in males (P <0.001), contrary to the values of GE was not different between genders. Individuals with higher circulating levels of TNF-&#945; also showed higher body weight, waist circumference, waist/hip ratio, fat-free mass, systolic and diastolic blood pressure, glucose and uric acid levels, and on the other hand, smaller values of percentage of total fat, total cholesterol and HDL- and LDL-c (P <0.05). Individuals with higher circulating levels of IL-6 showed higher values of body weight, BMI, truncal fat, fat-free mass, systolic and diastolic blood pressure, HDL-c and uric acid, on the other hand, lower values of percentage of total fat, biceps and triceps skinfold, triglycerides, total cholesterol and LDL-c (P <0.05). The GE of this cytokine presented inverse concentrations of IL-6 in the plasm (P = 0.03). Individuals with higher TNF-&#945; consumed more calories, protein, copper and fiber, but less iron in the diet (P<0.05). Those with higher IL-6 consumed more calories, macronutrients, fiber, iron and copper (P<0.05). By simple regression analysis, we found that the GE of ICAM1, the R1IL- 1 and IL-18 had a positive predictive effect on circulating levels of TNF-&#945; (P<0.05 ). As for IL-6, body weight, fat-free mass, truncal adiposity, the systolic and diastolic blood pressure, levels of uric acid, glucose and LDL-c had a positive predictive effect, unlike percentage of total fat, circulating levels of total cholesterol and LDL-c and HDL-c, besides the concentration of selenium in nails, which had a negative predictive effect. Individuals who are positioned in the two last tertiles (3.48 to 5.08 pg / mL and &#8805; 5.9 pg / mL) of the distribution of circulating levels of TNF-&#945;, compared with those in the lowest tertile (<3.48 pg/mL) showed a significant increase the chances of having abdominal obesity (P <0.05). In the same way, those who werw in the last tertile, compared with those in smaller, showed a significant increase in the chances of having low levels of HDL-c. When the same analysis was performed for IL-6, it was found that individuals who were in the two largest tertiles (3.43 to 4.86 pg / mL and &#8805; 4.87 pg / mL) of the distribution ofcirculating levels of this cytokine, compared with those in lowest tertile (<3.43 pg / mL) showed significant increase in serum concentrations likely to have decreased HDL-c. This study contributes to better understanding of associations between markers of interest (TNF-&#945; and IL-6) and the risk factors for the occurrence of diseases associated with MS. Moreover, this study revealed the association of mRNA expression of some inflammatory markers in PBMC with important variables in the surrounding context of metabolic diseases, even in healthy adults.