Impala, a transposon from Fusarium oxysporum, is active in the genome of Penicillium griseoroseum

• Autor(a) principal: Queiroz, Marisa Vieira de
• Data de Publicação: 2002
• Outros Autores: Daboussi, Marie-Josée
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1111/j.1574-6968.2003.tb11535.x; http://www.locus.ufv.br/handle/123456789/13890
• topic: Transposon impala; Heterologous transposition; Filamentous fungus; Penicillium griseoroseum

An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD gene and to reinsert at new genomic positions. As in the original host, impala inserts into a TA site and footprints left by impala excisions are generally 5 bp. The fact that impala is able to transpose in P. griseoroseum offers the opportunity to develop a gene-tagging system based on this element with the objective to detect and clone genes related in pectinase production.