Ação in vitro de fungos das espécies Duddingtonia flagrans, Monacrosporium sinense e Pochonia chlamydosporia sobre ovos de Fasciola hepatica e Schistosoma mansoni

• Autor(a) principal: Braga, Fábio Ribeiro
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4943
• topic: Fungos nematófagos; Fasciola hepatica; Schistosoma mansoni; Nematophagous fungi; Fasciola hepatica; Schistosoma mansoni; CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA

The in vitro effects of four isolates of the nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium sinense (SF53) and Pochonia chlamydosporia (VC1 and VC4) on eggs of Fasciola hepatica and Schistosoma mansoni was evaluated in two assays (A and B). Eggs of F. hepatica (assay A) and S. mansoni (assay B) were incubated in Petri dishes with 2% water-agar inoculated with the grown fungal isolates and a control without fungus. After seven, 14 and 21 days post- inoculation, one hundred eggs were removed and classified according to the following parameters: type 1, lytic effect without morphological damage to eggshell; type 2, lytic effect with morphological alteration of embryo and eggshell; and type 3, lytic effect with morphological alteration of embryo and eggshell, with hyphal penetration and internal egg colonization. In assay A, D. flagrans (AC001) and M. sinense (SF53) showed results only for type-1 effect on F. hepatica eggs, but with no significant difference (p>0.01) between them. P. chlamydosporia (VC1 and VC4) showed percentage results for ovicidal activity of type-1, -2 and -3 effects on F. hepatica eggs, with type-3 effect of 12.8% (VC1) and 16.5% (VC4); 14.4% (VC1) and 18.7% (VC4), 20.1% (VC1) and 21.5% (VC4) at seven, 14 and 21 days respectively. At the end of assay A no difference was found in the action of VC1 and VC4 for type-1, -2 and -3 effects over the three studied periods. In assay B, D. flagrans (AC001) and M. sinense (SF53) showed percentage results only for type 1 effect on S. mansoni eggs, with no significant difference (p>0.01) between them. P. chlamydosporia showed ovicidal activity on S. mansoni eggs with percentage results for type-1, -2 and -3 effects showing type 3 effect of: 26.6% (VC1) and 17.2% (VC4); 25.6% (VC1) and 22.6% (VC4); 26.3% (VC1) and 23.0% (VC4) at seven, 14 and 21 days respectively. At the end of the assay B, no difference was found in the action between the isolates VC1 and VC4 for type-1, -2 and -3 effects as well. The results of the A and B in vitro assays showed that P. chlamydosporia (VC1 and VC4) negatively affected F. hepatica and S. mansoni eggs and can therefore be used as biological control agent for these helminths.