Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.envexpbot.2016.12.004 http://www.locus.ufv.br/handle/123456789/21515 |
Resumo: | Sucrose is a key metabolite in guard cell (GC) regulation and has been proposed to form a key metabolic connection between mesophyll cells and GCs. However, the importance of sucrose transport across the GC plasma membrane in stomatal movements remains to be determined. Here, we characterized transgenic tobacco plants expressing an antisense gene construct for the SUCROSE TRANSPORTER 1 (SUT1) driven by the GC specific promoter KST1. GCs of transgenic plants contained less sucrose, more starch and had increases in K+ content. Lower GC sucrose content was associated with lower stomatal conductance that, in turn, led to a lower transpiration rate and higher carbon isotope composition ratio compared to wild type plants. Although the transgenic plants showed reduced growth under optimal water supply condition, they showed improved growth compared to wild type plants under osmotic stress and demonstrated lower water consumption that was correlated with higher relative water content under water-deficit conditions. This demonstrates the desiccation postponement phenotype of these plants together with an improved tolerance to water deficit. Taken together, our results indicate that SUT1 has an important role in stomatal movements and suggest that genetic manipulation of GC sucrose transport could be used to obtain plants with greater water use efficiency. |
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Antunes, Werner CamargosDaloso, Danilo de MenezesPinheiro, Daniela PereiraWilliams, Thomas Christopher RhysLoureiro, Marcelo Ehlers2018-08-29T16:37:43Z2018-08-29T16:37:43Z2017-0300988472https://doi.org/10.1016/j.envexpbot.2016.12.004http://www.locus.ufv.br/handle/123456789/21515Sucrose is a key metabolite in guard cell (GC) regulation and has been proposed to form a key metabolic connection between mesophyll cells and GCs. However, the importance of sucrose transport across the GC plasma membrane in stomatal movements remains to be determined. Here, we characterized transgenic tobacco plants expressing an antisense gene construct for the SUCROSE TRANSPORTER 1 (SUT1) driven by the GC specific promoter KST1. GCs of transgenic plants contained less sucrose, more starch and had increases in K+ content. Lower GC sucrose content was associated with lower stomatal conductance that, in turn, led to a lower transpiration rate and higher carbon isotope composition ratio compared to wild type plants. Although the transgenic plants showed reduced growth under optimal water supply condition, they showed improved growth compared to wild type plants under osmotic stress and demonstrated lower water consumption that was correlated with higher relative water content under water-deficit conditions. This demonstrates the desiccation postponement phenotype of these plants together with an improved tolerance to water deficit. Taken together, our results indicate that SUT1 has an important role in stomatal movements and suggest that genetic manipulation of GC sucrose transport could be used to obtain plants with greater water use efficiency.engEnvironmental and Experimental Botanyv. 135, p. 73- 85, march 2017Elsevier B.V.info:eu-repo/semantics/openAccessDrought avoidanceGuard cellOsmorregulationTranspirationSucrose transportWater use efficiencyGuard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal openinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf1116434https://locus.ufv.br//bitstream/123456789/21515/1/artigo.pdfb7ccc7c036888b614a8536f83854942cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/21515/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg6416https://locus.ufv.br//bitstream/123456789/21515/3/artigo.pdf.jpgd7e0b9c287d815c575114d9f5ca703b6MD53123456789/215152018-08-29 23:00:37.954oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-08-30T02:00:37LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening |
title |
Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening |
spellingShingle |
Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening Antunes, Werner Camargos Drought avoidance Guard cell Osmorregulation Transpiration Sucrose transport Water use efficiency |
title_short |
Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening |
title_full |
Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening |
title_fullStr |
Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening |
title_full_unstemmed |
Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening |
title_sort |
Guard cell-specific down-regulation of the sucrose transporter SUT1 leads to improved water use efficiency and reveals the interplay between carbohydrate metabolism and K+ accumulation in the regulation of stomatal opening |
author |
Antunes, Werner Camargos |
author_facet |
Antunes, Werner Camargos Daloso, Danilo de Menezes Pinheiro, Daniela Pereira Williams, Thomas Christopher Rhys Loureiro, Marcelo Ehlers |
author_role |
author |
author2 |
Daloso, Danilo de Menezes Pinheiro, Daniela Pereira Williams, Thomas Christopher Rhys Loureiro, Marcelo Ehlers |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Antunes, Werner Camargos Daloso, Danilo de Menezes Pinheiro, Daniela Pereira Williams, Thomas Christopher Rhys Loureiro, Marcelo Ehlers |
dc.subject.pt-BR.fl_str_mv |
Drought avoidance Guard cell Osmorregulation Transpiration Sucrose transport Water use efficiency |
topic |
Drought avoidance Guard cell Osmorregulation Transpiration Sucrose transport Water use efficiency |
description |
Sucrose is a key metabolite in guard cell (GC) regulation and has been proposed to form a key metabolic connection between mesophyll cells and GCs. However, the importance of sucrose transport across the GC plasma membrane in stomatal movements remains to be determined. Here, we characterized transgenic tobacco plants expressing an antisense gene construct for the SUCROSE TRANSPORTER 1 (SUT1) driven by the GC specific promoter KST1. GCs of transgenic plants contained less sucrose, more starch and had increases in K+ content. Lower GC sucrose content was associated with lower stomatal conductance that, in turn, led to a lower transpiration rate and higher carbon isotope composition ratio compared to wild type plants. Although the transgenic plants showed reduced growth under optimal water supply condition, they showed improved growth compared to wild type plants under osmotic stress and demonstrated lower water consumption that was correlated with higher relative water content under water-deficit conditions. This demonstrates the desiccation postponement phenotype of these plants together with an improved tolerance to water deficit. Taken together, our results indicate that SUT1 has an important role in stomatal movements and suggest that genetic manipulation of GC sucrose transport could be used to obtain plants with greater water use efficiency. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-03 |
dc.date.accessioned.fl_str_mv |
2018-08-29T16:37:43Z |
dc.date.available.fl_str_mv |
2018-08-29T16:37:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.envexpbot.2016.12.004 http://www.locus.ufv.br/handle/123456789/21515 |
dc.identifier.issn.none.fl_str_mv |
00988472 |
identifier_str_mv |
00988472 |
url |
https://doi.org/10.1016/j.envexpbot.2016.12.004 http://www.locus.ufv.br/handle/123456789/21515 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 135, p. 73- 85, march 2017 |
dc.rights.driver.fl_str_mv |
Elsevier B.V. info:eu-repo/semantics/openAccess |
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Elsevier B.V. |
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openAccess |
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Environmental and Experimental Botany |
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Environmental and Experimental Botany |
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