Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos

Detalhes bibliográficos
Autor(a) principal: Bento, Cláudia Braga Pereira
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5296
Resumo: The increase in world population demands ever more food and consumer goods, with a consequent increase in the production of agroindustrial residues natives from logging, alcohol fuel plants and industries for beneficiary eligibility of agricultural products. Several studies have demonstrated the potential of white rot fungi to decompose of lignocellulosic substrates, but the use of such residues in ruminant diets has not been properly examined. The objective of this study was to determine the chemical composition and in vitro digestibility of agroindustrial residues (eucalyptus bark, sawdust, sugarcane bagasse, corn kernels, coffee bark, coconut fiber and lump of cotton) inoculated with white rot fungi (Pleurotus ostreatus and Lentinula edodes), and determine the activity of cellulase, xylanase and laccase in ruminal fluid incubated in vitro with corn kernels or sugarcane bagasse ground at different particle sizes. When the residues were treated with L. edodes the content of CP in eucalyptus bark increased 91 % and 78 % in the treatments added with rice bran or urea, respectively. The content of ADL decreased 70% in fructified sawdust added with rice bran compared to the controls. The NDF of sugarcane bagasse decreased 5 % when added with rice bran and 21 % when added with urea. When the residues were treated with P. ostreatus the levels of CEL and ADL of eucalyptus bark decreased 22 % and 137 %, respectively. With sawdust, the concentrations of NDF and ADF decreased 19 % and 27 %, respectively, after fruit body formation. The EE increased 402 % in sugarcane bagasse treated with P. ostreatus. With corn kernels, the content of ash increased 130 % compared to controls in the treatment showing fruit body formation. There was a reduction of 60 % in the LDA content of coffee barks after fructification. Fungus fructification in the coconut fiber reduced the levels of LDA and CEL in 25 % and 20 %, respectively. There were no significant differences between treatments for CP, ADF, CEL, HEM and ADL contents in the lump of cotton. However, there was significant difference in IVDMD between the enriched fructified substrates treated with L. edodes. An average increase 111 % and 98 % was observed for the fructified fungus added with rice bran or urea, respectively, compared to controls. The biggest increase in IVDMD after fructification in residues treated with P. ostreatus was obtained for the eucalyptus bark (200 %), followed by corn kernels (67 %) and sugar cane bagasse (13 %). The highest cellulase activity in corn kernels residues was obtained when using particles with a diameter of 0.6 mm. The xylanase activity was higher than the activity of cellulase for all treatments and sizes of particles tested. The particle size of 0.6 mm in the inoculated treatment yielded maximum xylanase activity after 48 hours of incubation (118.17 U/mL). The activity of cellulase in sugarcane bagasse was superior for all treatments and particle sizes when compared to corn kernels residues. The highest activity of xylanase (78.89 U/mL) in the fructified sugarcane bagasse was obtained after 48 hours of incubation and particles size of 0.6 mm. The changes in chemical composition and IVDMD showed that L. edodes (UFV 73) and P. ostreatus (PLO 06) could improve the nutritional quality of ruminant rations by increasing the content of CP and IVDMD and reducing the levels of NDF, ADF and ADL of agroindustrial residues, allowing its use in ruminant rations.
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spelling Bento, Cláudia Braga Pereirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4137421E5Kasuya, Maria Catarina Megumihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721444T5Rodrigues, Marcelo Teixeirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788161Y5Mantovani, Hilário Cuquettohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727026Z7Passos, Flávia Maria Lopeshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781817D3Nascimento, Antonio Galvão dohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797432E82015-03-26T13:51:43Z2008-09-252015-03-26T13:51:43Z2008-04-30BENTO, Cláudia Braga Pereira. Effect of white rot fungi on the nutritional quality of lignocellulosic agroindustrial residues. 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/5296The increase in world population demands ever more food and consumer goods, with a consequent increase in the production of agroindustrial residues natives from logging, alcohol fuel plants and industries for beneficiary eligibility of agricultural products. Several studies have demonstrated the potential of white rot fungi to decompose of lignocellulosic substrates, but the use of such residues in ruminant diets has not been properly examined. The objective of this study was to determine the chemical composition and in vitro digestibility of agroindustrial residues (eucalyptus bark, sawdust, sugarcane bagasse, corn kernels, coffee bark, coconut fiber and lump of cotton) inoculated with white rot fungi (Pleurotus ostreatus and Lentinula edodes), and determine the activity of cellulase, xylanase and laccase in ruminal fluid incubated in vitro with corn kernels or sugarcane bagasse ground at different particle sizes. When the residues were treated with L. edodes the content of CP in eucalyptus bark increased 91 % and 78 % in the treatments added with rice bran or urea, respectively. The content of ADL decreased 70% in fructified sawdust added with rice bran compared to the controls. The NDF of sugarcane bagasse decreased 5 % when added with rice bran and 21 % when added with urea. When the residues were treated with P. ostreatus the levels of CEL and ADL of eucalyptus bark decreased 22 % and 137 %, respectively. With sawdust, the concentrations of NDF and ADF decreased 19 % and 27 %, respectively, after fruit body formation. The EE increased 402 % in sugarcane bagasse treated with P. ostreatus. With corn kernels, the content of ash increased 130 % compared to controls in the treatment showing fruit body formation. There was a reduction of 60 % in the LDA content of coffee barks after fructification. Fungus fructification in the coconut fiber reduced the levels of LDA and CEL in 25 % and 20 %, respectively. There were no significant differences between treatments for CP, ADF, CEL, HEM and ADL contents in the lump of cotton. However, there was significant difference in IVDMD between the enriched fructified substrates treated with L. edodes. An average increase 111 % and 98 % was observed for the fructified fungus added with rice bran or urea, respectively, compared to controls. The biggest increase in IVDMD after fructification in residues treated with P. ostreatus was obtained for the eucalyptus bark (200 %), followed by corn kernels (67 %) and sugar cane bagasse (13 %). The highest cellulase activity in corn kernels residues was obtained when using particles with a diameter of 0.6 mm. The xylanase activity was higher than the activity of cellulase for all treatments and sizes of particles tested. The particle size of 0.6 mm in the inoculated treatment yielded maximum xylanase activity after 48 hours of incubation (118.17 U/mL). The activity of cellulase in sugarcane bagasse was superior for all treatments and particle sizes when compared to corn kernels residues. The highest activity of xylanase (78.89 U/mL) in the fructified sugarcane bagasse was obtained after 48 hours of incubation and particles size of 0.6 mm. The changes in chemical composition and IVDMD showed that L. edodes (UFV 73) and P. ostreatus (PLO 06) could improve the nutritional quality of ruminant rations by increasing the content of CP and IVDMD and reducing the levels of NDF, ADF and ADL of agroindustrial residues, allowing its use in ruminant rations.O aumento da população mundial demanda cada vez mais alimentos e bens de consumo, com conseqüente aumento na produção de resíduos agroindustriais oriundos de madeireiras, usinas de álcool combustível e indústrias de beneficiamento de produtos agrícolas. Diversos estudos têm demonstrado o potencial de fungos da podridão branca em decompor substratos ligninocelulósicos, mas a utilização desses resíduos na alimentação de ruminantes ainda tem sido pouco explorada. O objetivo deste trabalho foi determinar a composição bromatológica e a digestibilidade in vitro de resíduos agroindustriais (casca de eucalipto, serragem de eucalipto, bagaço de cana-de-açúcar, sabugo de milho, casca de café, fibra de coco e casca de caroço de algodão desengordurado) inoculados com fungos causadores da podridão branca (Pleurotus ostreatus e Lentinula edodes), assim como determinar a atividade enzimática de celulase, xilanase e lacase em três diferentes tamanhos de partículas dos resíduos sabugo de milho e bagaço de cana-de-açúcar incubados com líquido ruminal in vitro, visando avaliar o potencial hidrolítico das comunidades microbianas do rúmen. Quando os resíduos foram tratados com L. edodes o teor de PB do resíduo casca de eucalipto aumentou 91 % e 78 % no tratamento enriquecido com farelo de arroz e uréia, respectivamente. O teor de LDA diminuiu 70 % na serragem de eucalipto frutificada e enriquecida com farelo de arroz comparada ao controle. A FDN do resíduo bagaço de cana diminuiu 5 % quando enriquecido com farelo e 21 % quando enriquecido com uréia. Quando os resíduos foram tratados com P. ostreatus os teores de CEL e LDA da casca de eucalipto diminuíram 22 % e 137 %, respectivamente, após o tratamento. Na serragem de eucalipto as concentrações de FDN e FDA diminuíram 19 % e 27 % no tratamento frutificado, respectivamente. O EE aumentou 402 % no tratamento frutificado no bagaço. No sabugo o conteúdo de cinzas aumentou 130 % no tratamento frutificado em relação ao controle. Houve redução de 60 % no teor de LDA da casca de café após a frutificação. A frutificação fúngica reduziu os teores de LDA e CEL em 25 % e 20 %, respectivamente na fibra de coco. Não houve diferenças significativas entre os tratamentos para os teores de PB, FDA, CEL, HEM e LDA no resíduo casca de caroço de algodão desengordurado. Houve diferença significativa na DIVMS entre os enriquecimentos dos resíduos tratados com L. edodes, tendo sido observado aumento médio de 111 % e 98 % quando frutificado e adicionado de farelo de arroz e uréia, respectivamente, em relação ao controle O maior incremento de DIVMS no tratamento frutificado nos resíduos tratados com P. ostreatus foi obtido para a casca de eucalipto (200 %), sabugo de milho (67 %) e bagaço de cana-de-açúcar (13 %). As maiores atividades de celulase no resíduo sabugo de milho foram obtidas quando se utilizou partículas com 0,6 mm de diâmetro. A atividade de xilanase foi maior quando comparada à atividade de celulase em todos os tratamentos e tamanhos de partículas testados. O tamanho de partícula 0,6 mm no tratamento inoculado foi o que apresentou atividade máxima de xilanase após 48 horas de incubação (118,17 U/mL). A atividade de celulase no resíduo bagaço de cana-de-açúcar foi superior em todos os tratamentos e tamanhos de partículas quando comparado ao resíduo sabugo de milho. O tamanho de partícula 0,6 mm apresentou as maiores concentrações de xilanase no bagaço de cana-de-açúcar, tendo sido observado valor máximo após 48 horas de incubação de 78,89 U/mL no tratamento frutificado. As mudanças na composição química e na DIVMS indicam que L. edodes (UFV 73) e P. ostreatus (PLO 06) melhoram o valor nutricional dos resíduos tratados, aumentando o teor de PB e DIVMS e reduzindo os teores de FDN, FDA e LDA dos resíduos agroindustriais, com possibilidade de utilização na alimentação de ruminantes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Microbiologia AgrícolaUFVBRAssociações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesseResíduos ligninocelulósicosFungos da podridão brancaQualidade nutricionalDigestibilidadeLignocellulosic residuesWhite rot fungiNutritional qualityDigestibilityCNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADAEfeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicosEffect of white rot fungi on the nutritional quality of lignocellulosic agroindustrial residuesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL01 - capa_abstract.pdfapplication/pdf74835https://locus.ufv.br//bitstream/123456789/5296/1/01%20-%20capa_abstract.pdf716f5bf894e92efbf5c4b42ff95351adMD51TEXT01 - capa_abstract.pdf.txt01 - capa_abstract.pdf.txtExtracted texttext/plain8745https://locus.ufv.br//bitstream/123456789/5296/2/01%20-%20capa_abstract.pdf.txtcc95e61a59b88f2cb4aa16b5349eeadfMD52THUMBNAIL01 - capa_abstract.pdf.jpg01 - capa_abstract.pdf.jpgIM Thumbnailimage/jpeg3736https://locus.ufv.br//bitstream/123456789/5296/3/01%20-%20capa_abstract.pdf.jpgf133dc746f88cf9ac581b154bb52e32fMD53123456789/52962016-04-10 23:17:26.298oai:locus.ufv.br:123456789/5296Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:17:26LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos
dc.title.alternative.eng.fl_str_mv Effect of white rot fungi on the nutritional quality of lignocellulosic agroindustrial residues
title Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos
spellingShingle Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos
Bento, Cláudia Braga Pereira
Resíduos ligninocelulósicos
Fungos da podridão branca
Qualidade nutricional
Digestibilidade
Lignocellulosic residues
White rot fungi
Nutritional quality
Digestibility
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA
title_short Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos
title_full Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos
title_fullStr Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos
title_full_unstemmed Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos
title_sort Efeito de fungos da podridão branca sobre a qualidade nutricional de resíduos agroindustriais ligninocelulósicos
author Bento, Cláudia Braga Pereira
author_facet Bento, Cláudia Braga Pereira
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4137421E5
dc.contributor.author.fl_str_mv Bento, Cláudia Braga Pereira
dc.contributor.advisor-co1.fl_str_mv Kasuya, Maria Catarina Megumi
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721444T5
dc.contributor.advisor-co2.fl_str_mv Rodrigues, Marcelo Teixeira
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788161Y5
dc.contributor.advisor1.fl_str_mv Mantovani, Hilário Cuquetto
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727026Z7
dc.contributor.referee1.fl_str_mv Passos, Flávia Maria Lopes
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781817D3
dc.contributor.referee2.fl_str_mv Nascimento, Antonio Galvão do
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797432E8
contributor_str_mv Kasuya, Maria Catarina Megumi
Rodrigues, Marcelo Teixeira
Mantovani, Hilário Cuquetto
Passos, Flávia Maria Lopes
Nascimento, Antonio Galvão do
dc.subject.por.fl_str_mv Resíduos ligninocelulósicos
Fungos da podridão branca
Qualidade nutricional
Digestibilidade
topic Resíduos ligninocelulósicos
Fungos da podridão branca
Qualidade nutricional
Digestibilidade
Lignocellulosic residues
White rot fungi
Nutritional quality
Digestibility
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA
dc.subject.eng.fl_str_mv Lignocellulosic residues
White rot fungi
Nutritional quality
Digestibility
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA
description The increase in world population demands ever more food and consumer goods, with a consequent increase in the production of agroindustrial residues natives from logging, alcohol fuel plants and industries for beneficiary eligibility of agricultural products. Several studies have demonstrated the potential of white rot fungi to decompose of lignocellulosic substrates, but the use of such residues in ruminant diets has not been properly examined. The objective of this study was to determine the chemical composition and in vitro digestibility of agroindustrial residues (eucalyptus bark, sawdust, sugarcane bagasse, corn kernels, coffee bark, coconut fiber and lump of cotton) inoculated with white rot fungi (Pleurotus ostreatus and Lentinula edodes), and determine the activity of cellulase, xylanase and laccase in ruminal fluid incubated in vitro with corn kernels or sugarcane bagasse ground at different particle sizes. When the residues were treated with L. edodes the content of CP in eucalyptus bark increased 91 % and 78 % in the treatments added with rice bran or urea, respectively. The content of ADL decreased 70% in fructified sawdust added with rice bran compared to the controls. The NDF of sugarcane bagasse decreased 5 % when added with rice bran and 21 % when added with urea. When the residues were treated with P. ostreatus the levels of CEL and ADL of eucalyptus bark decreased 22 % and 137 %, respectively. With sawdust, the concentrations of NDF and ADF decreased 19 % and 27 %, respectively, after fruit body formation. The EE increased 402 % in sugarcane bagasse treated with P. ostreatus. With corn kernels, the content of ash increased 130 % compared to controls in the treatment showing fruit body formation. There was a reduction of 60 % in the LDA content of coffee barks after fructification. Fungus fructification in the coconut fiber reduced the levels of LDA and CEL in 25 % and 20 %, respectively. There were no significant differences between treatments for CP, ADF, CEL, HEM and ADL contents in the lump of cotton. However, there was significant difference in IVDMD between the enriched fructified substrates treated with L. edodes. An average increase 111 % and 98 % was observed for the fructified fungus added with rice bran or urea, respectively, compared to controls. The biggest increase in IVDMD after fructification in residues treated with P. ostreatus was obtained for the eucalyptus bark (200 %), followed by corn kernels (67 %) and sugar cane bagasse (13 %). The highest cellulase activity in corn kernels residues was obtained when using particles with a diameter of 0.6 mm. The xylanase activity was higher than the activity of cellulase for all treatments and sizes of particles tested. The particle size of 0.6 mm in the inoculated treatment yielded maximum xylanase activity after 48 hours of incubation (118.17 U/mL). The activity of cellulase in sugarcane bagasse was superior for all treatments and particle sizes when compared to corn kernels residues. The highest activity of xylanase (78.89 U/mL) in the fructified sugarcane bagasse was obtained after 48 hours of incubation and particles size of 0.6 mm. The changes in chemical composition and IVDMD showed that L. edodes (UFV 73) and P. ostreatus (PLO 06) could improve the nutritional quality of ruminant rations by increasing the content of CP and IVDMD and reducing the levels of NDF, ADF and ADL of agroindustrial residues, allowing its use in ruminant rations.
publishDate 2008
dc.date.available.fl_str_mv 2008-09-25
2015-03-26T13:51:43Z
dc.date.issued.fl_str_mv 2008-04-30
dc.date.accessioned.fl_str_mv 2015-03-26T13:51:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv BENTO, Cláudia Braga Pereira. Effect of white rot fungi on the nutritional quality of lignocellulosic agroindustrial residues. 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5296
identifier_str_mv BENTO, Cláudia Braga Pereira. Effect of white rot fungi on the nutritional quality of lignocellulosic agroindustrial residues. 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.
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dc.publisher.program.fl_str_mv Mestrado em Microbiologia Agrícola
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dc.publisher.department.fl_str_mv Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse
publisher.none.fl_str_mv Universidade Federal de Viçosa
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