Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.riam.2017.04.001 http://www.locus.ufv.br/handle/123456789/18594 |
Resumo: | Exposure of the nematophagous fungus Pochonia chlamydosporia to solar radiation and elevated temperatures before being incorporated into the soil can reduce its survival and efficiency as biocontrol agent. A field experiment was carried out to assess the effect of the exposure period on the viability of P. chlamydosporia applied on the soil surface. A commercial bionematicide based on P. chlamydosporia was sprayed on soil, and soil samples were collected before and at 0, 30, 60, 90, 120, and 150 min after fungal application. Relative humidity (RH), the irradiance (IR), air temperature (AT), and soil temperature (ST) were recorded. The number of P. chlamydosporia colony forming units (CFUs) was evaluated after 20 days of incubation. P. chlamydosporia survival decreased over the time of exposure on the soil surface. Overall, the number of CFUs decreased by more than 90% at 150 min after application. Exposure to RH ≥61%, ST and AT between 25–35 ◦ C and 19–29 ◦ C, and IR between 1172 and 2126 mol of photons m −2 s −1 induced a negative exponential effect on the survival of the fungus over the time. Exposure to climatic conditions on the soil surface reduces P. chlamydosporia viability. |
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Fernandes, Rafael HenriqueLopes, Everaldo AntônioBorges, Darlan FerreiraBontempo, Amanda FerreiraZanuncio, José ColaSerrão, José Eduardo2018-04-03T11:21:53Z2018-04-03T11:21:53Z2017-09-2711301406https://doi.org/10.1016/j.riam.2017.04.001http://www.locus.ufv.br/handle/123456789/18594Exposure of the nematophagous fungus Pochonia chlamydosporia to solar radiation and elevated temperatures before being incorporated into the soil can reduce its survival and efficiency as biocontrol agent. A field experiment was carried out to assess the effect of the exposure period on the viability of P. chlamydosporia applied on the soil surface. A commercial bionematicide based on P. chlamydosporia was sprayed on soil, and soil samples were collected before and at 0, 30, 60, 90, 120, and 150 min after fungal application. Relative humidity (RH), the irradiance (IR), air temperature (AT), and soil temperature (ST) were recorded. The number of P. chlamydosporia colony forming units (CFUs) was evaluated after 20 days of incubation. P. chlamydosporia survival decreased over the time of exposure on the soil surface. Overall, the number of CFUs decreased by more than 90% at 150 min after application. Exposure to RH ≥61%, ST and AT between 25–35 ◦ C and 19–29 ◦ C, and IR between 1172 and 2126 mol of photons m −2 s −1 induced a negative exponential effect on the survival of the fungus over the time. Exposure to climatic conditions on the soil surface reduces P. chlamydosporia viability.La exposición del hongo nematófago Pochonia chlamydosporia a la radiación solar y latemperatura elevada antes de ser incorporado al suelo puede reducir su supervivencia y eficiencia como agente de biocontrol. Se realizó un experimento de campo para evaluar el efecto del período de exposición a condiciones ambientales sobre la viabilidad de P. chlamydosporia en la superficie del suelo. Se pulverizó sobre el suelo un bionematicida comercial hecho a base de P. chlamydosporia y se recogieron muestras de suelo antes y después de 0, 30, 60, 90, 120 y 150 min tras la aplicación del hongo. Se registraron la humedad relativa (HR), la irradiación (IR), la temperatura del aire (TA) y la temperatura del suelo (TS). Se evaluó el número de unidades formadoras de colonias (UFC) de P. chlamydosporia después de 20 días de incubación. La supervivencia de P. chlamydosporia disminuyó durante el tiempo de exposición en la superficie del suelo. En general, el número de UFC disminuyó en más de un 90% a los 150 min después de la aplicación. La exposición a HR ≥ 61%, TS y TA entre 25-35 ◦ C y 19-29 ◦ C, e IR entre 1.172 y 2.126 mol de fotones m −2 s −1 indujo un efecto exponencial negativo en la supervivencia del hongo. La exposición a las condiciones climáticas en la superficie del suelo reduce la viabilidad de P. chlamydosporia.engRevista Iberoamericana de Micologíav. 34, Issue 4, p. 241-245, October–December 2017Asociación Española de Micología. Published by Elsevier España, S.L.U. All rights reserved.info:eu-repo/semantics/openAccessBiological controlIrradianceRelative humidityUltraviolet irradiationViabilitySurvival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf848774https://locus.ufv.br//bitstream/123456789/18594/1/artigo.pdf16e617bba91db60e3f03d2e9c22e1f68MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18594/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg3428https://locus.ufv.br//bitstream/123456789/18594/3/artigo.pdf.jpg3cede88f69b473713635991b5dfd5741MD53123456789/185942018-04-03 23:00:38.886oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-04T02:00:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions |
title |
Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions |
spellingShingle |
Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions Fernandes, Rafael Henrique Biological control Irradiance Relative humidity Ultraviolet irradiation Viability |
title_short |
Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions |
title_full |
Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions |
title_fullStr |
Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions |
title_full_unstemmed |
Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions |
title_sort |
Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions |
author |
Fernandes, Rafael Henrique |
author_facet |
Fernandes, Rafael Henrique Lopes, Everaldo Antônio Borges, Darlan Ferreira Bontempo, Amanda Ferreira Zanuncio, José Cola Serrão, José Eduardo |
author_role |
author |
author2 |
Lopes, Everaldo Antônio Borges, Darlan Ferreira Bontempo, Amanda Ferreira Zanuncio, José Cola Serrão, José Eduardo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Rafael Henrique Lopes, Everaldo Antônio Borges, Darlan Ferreira Bontempo, Amanda Ferreira Zanuncio, José Cola Serrão, José Eduardo |
dc.subject.pt-BR.fl_str_mv |
Biological control Irradiance Relative humidity Ultraviolet irradiation Viability |
topic |
Biological control Irradiance Relative humidity Ultraviolet irradiation Viability |
description |
Exposure of the nematophagous fungus Pochonia chlamydosporia to solar radiation and elevated temperatures before being incorporated into the soil can reduce its survival and efficiency as biocontrol agent. A field experiment was carried out to assess the effect of the exposure period on the viability of P. chlamydosporia applied on the soil surface. A commercial bionematicide based on P. chlamydosporia was sprayed on soil, and soil samples were collected before and at 0, 30, 60, 90, 120, and 150 min after fungal application. Relative humidity (RH), the irradiance (IR), air temperature (AT), and soil temperature (ST) were recorded. The number of P. chlamydosporia colony forming units (CFUs) was evaluated after 20 days of incubation. P. chlamydosporia survival decreased over the time of exposure on the soil surface. Overall, the number of CFUs decreased by more than 90% at 150 min after application. Exposure to RH ≥61%, ST and AT between 25–35 ◦ C and 19–29 ◦ C, and IR between 1172 and 2126 mol of photons m −2 s −1 induced a negative exponential effect on the survival of the fungus over the time. Exposure to climatic conditions on the soil surface reduces P. chlamydosporia viability. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-09-27 |
dc.date.accessioned.fl_str_mv |
2018-04-03T11:21:53Z |
dc.date.available.fl_str_mv |
2018-04-03T11:21:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.riam.2017.04.001 http://www.locus.ufv.br/handle/123456789/18594 |
dc.identifier.issn.none.fl_str_mv |
11301406 |
identifier_str_mv |
11301406 |
url |
https://doi.org/10.1016/j.riam.2017.04.001 http://www.locus.ufv.br/handle/123456789/18594 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 34, Issue 4, p. 241-245, October–December 2017 |
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Asociación Española de Micología. Published by Elsevier España, S.L.U. All rights reserved. info:eu-repo/semantics/openAccess |
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Asociación Española de Micología. Published by Elsevier España, S.L.U. All rights reserved. |
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openAccess |
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application/pdf |
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Revista Iberoamericana de Micología |
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Revista Iberoamericana de Micología |
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Universidade Federal de Viçosa (UFV) |
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UFV |
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LOCUS Repositório Institucional da UFV |
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