Lipoxygenase activities during development of root and nodule of soybean

Detalhes bibliográficos
Autor(a) principal: Oliveira, Maria Goreti de Almeida
Data de Publicação: 2004
Outros Autores: Moreira, Maurilio Alves, Junghans, Tatiana Góes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S0100-204X2004000700002
https://locus.ufv.br//handle/123456789/26846
Resumo: The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.
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spelling Lipoxygenase activities during development of root and nodule of soybeanGlycine maxTriple-null linesLinhagem triplo nulaNodulaçãoNodulationThe objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.O objetivo deste trabalho foi avaliar lipoxigenases de raízes e nódulos de plantas de soja da variedade Doko e da linhagem triplo nula derivada, desprovida das lipoxigenases da semente, com e sem inoculação de Bradyrhizobium elkanii. A atividade de lipoxigenase foi avaliada em raízes coletadas aos 3, 5, 9, 13, 18 e 28 dias após a inoculação e em nódulos coletados aos 13, 18 e 28 dias após a inoculação. Os perfis de pH-atividade de raiz e nódulo sugerem que o "pool" de lipoxigenases expresso nesses órgãos não difere nos dois genótipos. A atividade de lipoxigenases nas raízes de Doko e Doko triplo nula, com e sem inoculação, declinou com o passar do tempo. A maior atividade de lipoxigenases no início de formação da raiz sugere o envolvimento desta enzima no crescimento e desenvolvimento deste órgão. Nos nódulos houve um aumento acentuado na atividade de lipoxigenase aos 28 dias após a inoculação. Dois grupos de mobilidade com aproximadamente 94 e 97 kDa foram encontrados nos "immunoblottings" para lipoxigenases de raiz e de nódulo.Pesquisa Agropecuária Brasileira2019-09-04T14:37:21Z2019-09-04T14:37:21Z2004-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1678-3921http://dx.doi.org/10.1590/S0100-204X2004000700002https://locus.ufv.br//handle/123456789/26846engv. 39, n. 07, p. 625- 630, jul. 2004Oliveira, Maria Goreti de AlmeidaMoreira, Maurilio AlvesJunghans, Tatiana Góesinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:40:54Zoai:locus.ufv.br:123456789/26846Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:40:54LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Lipoxygenase activities during development of root and nodule of soybean
title Lipoxygenase activities during development of root and nodule of soybean
spellingShingle Lipoxygenase activities during development of root and nodule of soybean
Oliveira, Maria Goreti de Almeida
Glycine max
Triple-null lines
Linhagem triplo nula
Nodulação
Nodulation
title_short Lipoxygenase activities during development of root and nodule of soybean
title_full Lipoxygenase activities during development of root and nodule of soybean
title_fullStr Lipoxygenase activities during development of root and nodule of soybean
title_full_unstemmed Lipoxygenase activities during development of root and nodule of soybean
title_sort Lipoxygenase activities during development of root and nodule of soybean
author Oliveira, Maria Goreti de Almeida
author_facet Oliveira, Maria Goreti de Almeida
Moreira, Maurilio Alves
Junghans, Tatiana Góes
author_role author
author2 Moreira, Maurilio Alves
Junghans, Tatiana Góes
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira, Maria Goreti de Almeida
Moreira, Maurilio Alves
Junghans, Tatiana Góes
dc.subject.por.fl_str_mv Glycine max
Triple-null lines
Linhagem triplo nula
Nodulação
Nodulation
topic Glycine max
Triple-null lines
Linhagem triplo nula
Nodulação
Nodulation
description The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.
publishDate 2004
dc.date.none.fl_str_mv 2004-07
2019-09-04T14:37:21Z
2019-09-04T14:37:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1678-3921
http://dx.doi.org/10.1590/S0100-204X2004000700002
https://locus.ufv.br//handle/123456789/26846
identifier_str_mv 1678-3921
url http://dx.doi.org/10.1590/S0100-204X2004000700002
https://locus.ufv.br//handle/123456789/26846
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 39, n. 07, p. 625- 630, jul. 2004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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