Lipoxygenase activities during development of root and nodule of soybean
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/S0100-204X2004000700002 https://locus.ufv.br//handle/123456789/26846 |
Resumo: | The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa. |
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LOCUS Repositório Institucional da UFV |
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Lipoxygenase activities during development of root and nodule of soybeanGlycine maxTriple-null linesLinhagem triplo nulaNodulaçãoNodulationThe objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.O objetivo deste trabalho foi avaliar lipoxigenases de raízes e nódulos de plantas de soja da variedade Doko e da linhagem triplo nula derivada, desprovida das lipoxigenases da semente, com e sem inoculação de Bradyrhizobium elkanii. A atividade de lipoxigenase foi avaliada em raízes coletadas aos 3, 5, 9, 13, 18 e 28 dias após a inoculação e em nódulos coletados aos 13, 18 e 28 dias após a inoculação. Os perfis de pH-atividade de raiz e nódulo sugerem que o "pool" de lipoxigenases expresso nesses órgãos não difere nos dois genótipos. A atividade de lipoxigenases nas raízes de Doko e Doko triplo nula, com e sem inoculação, declinou com o passar do tempo. A maior atividade de lipoxigenases no início de formação da raiz sugere o envolvimento desta enzima no crescimento e desenvolvimento deste órgão. Nos nódulos houve um aumento acentuado na atividade de lipoxigenase aos 28 dias após a inoculação. Dois grupos de mobilidade com aproximadamente 94 e 97 kDa foram encontrados nos "immunoblottings" para lipoxigenases de raiz e de nódulo.Pesquisa Agropecuária Brasileira2019-09-04T14:37:21Z2019-09-04T14:37:21Z2004-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1678-3921http://dx.doi.org/10.1590/S0100-204X2004000700002https://locus.ufv.br//handle/123456789/26846engv. 39, n. 07, p. 625- 630, jul. 2004Oliveira, Maria Goreti de AlmeidaMoreira, Maurilio AlvesJunghans, Tatiana Góesinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:40:54Zoai:locus.ufv.br:123456789/26846Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:40:54LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Lipoxygenase activities during development of root and nodule of soybean |
title |
Lipoxygenase activities during development of root and nodule of soybean |
spellingShingle |
Lipoxygenase activities during development of root and nodule of soybean Oliveira, Maria Goreti de Almeida Glycine max Triple-null lines Linhagem triplo nula Nodulação Nodulation |
title_short |
Lipoxygenase activities during development of root and nodule of soybean |
title_full |
Lipoxygenase activities during development of root and nodule of soybean |
title_fullStr |
Lipoxygenase activities during development of root and nodule of soybean |
title_full_unstemmed |
Lipoxygenase activities during development of root and nodule of soybean |
title_sort |
Lipoxygenase activities during development of root and nodule of soybean |
author |
Oliveira, Maria Goreti de Almeida |
author_facet |
Oliveira, Maria Goreti de Almeida Moreira, Maurilio Alves Junghans, Tatiana Góes |
author_role |
author |
author2 |
Moreira, Maurilio Alves Junghans, Tatiana Góes |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Oliveira, Maria Goreti de Almeida Moreira, Maurilio Alves Junghans, Tatiana Góes |
dc.subject.por.fl_str_mv |
Glycine max Triple-null lines Linhagem triplo nula Nodulação Nodulation |
topic |
Glycine max Triple-null lines Linhagem triplo nula Nodulação Nodulation |
description |
The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-07 2019-09-04T14:37:21Z 2019-09-04T14:37:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
1678-3921 http://dx.doi.org/10.1590/S0100-204X2004000700002 https://locus.ufv.br//handle/123456789/26846 |
identifier_str_mv |
1678-3921 |
url |
http://dx.doi.org/10.1590/S0100-204X2004000700002 https://locus.ufv.br//handle/123456789/26846 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 39, n. 07, p. 625- 630, jul. 2004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1817559951468396544 |