From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.jspr.2018.10.004 http://www.locus.ufv.br/handle/123456789/23604 |
Resumo: | The aim of this work was to evaluate the insect mobility patterns of phosphine-resistant and -susceptible adults of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) after exposure to phosphine. Exposure bioassays were carried out at two concentrations, 1000 and 3000 ppm, while adults were observed every 15 min, for a total period of 90 min. During this observation interval, adults were visually classified as active (able to walk normally), partially immobilized (not able to walk, but showing a minimal movement), or completely immobilized (no visible movement). After the observation period, all adults were placed in a phosphine-free environment, and they again were classified as active, partially immobilized or completely immobilized. At 1000 ppm, the majority of adults of the susceptible T. castaneum population were quickly immobilized after a 15 min observation period, while in contrast, the majority of adults of the resistant T. castaneum population were still active after the termination of the 90 min interval. At 3000 ppm, the percentage of immobilized susceptible adults was increased at the 15 min observation period, while the majority of resistant adults were immobilized only after 90 min. In the post-exposure period, the vast majority of the susceptible adults were dead. In contrast, most resistant adults recovered, regardless of the concentration that had been exposed. The results of this study delineate major differences in movement in phosphine-resistant and -susceptible T. castaneum strains, and can be applied as a quick diagnostic bioassay for the evaluation of resistance to phosphine in stored product insects. |
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Athanassiou, Christos G.Kavallieratos, Nickolas G.Brabec, Daniel L.Oppert, BrendaCampbell, James F.Guedes, Raul N. C.2019-02-20T11:04:03Z2019-02-20T11:04:03Z2019-010022-474Xhttps://doi.org/10.1016/j.jspr.2018.10.004http://www.locus.ufv.br/handle/123456789/23604The aim of this work was to evaluate the insect mobility patterns of phosphine-resistant and -susceptible adults of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) after exposure to phosphine. Exposure bioassays were carried out at two concentrations, 1000 and 3000 ppm, while adults were observed every 15 min, for a total period of 90 min. During this observation interval, adults were visually classified as active (able to walk normally), partially immobilized (not able to walk, but showing a minimal movement), or completely immobilized (no visible movement). After the observation period, all adults were placed in a phosphine-free environment, and they again were classified as active, partially immobilized or completely immobilized. At 1000 ppm, the majority of adults of the susceptible T. castaneum population were quickly immobilized after a 15 min observation period, while in contrast, the majority of adults of the resistant T. castaneum population were still active after the termination of the 90 min interval. At 3000 ppm, the percentage of immobilized susceptible adults was increased at the 15 min observation period, while the majority of resistant adults were immobilized only after 90 min. In the post-exposure period, the vast majority of the susceptible adults were dead. In contrast, most resistant adults recovered, regardless of the concentration that had been exposed. The results of this study delineate major differences in movement in phosphine-resistant and -susceptible T. castaneum strains, and can be applied as a quick diagnostic bioassay for the evaluation of resistance to phosphine in stored product insects.engJournal of Stored Products ResearchVolume 80, Pages 28- 33, January 20192018 Elsevier Ltd. All rights reserved.info:eu-repo/semantics/openAccessDiagnostic indicatorInsect movementMortalityPhosphineRed flour beetleTribolium castaneumInsecticide resistanceFrom immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistanceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf300831https://locus.ufv.br//bitstream/123456789/23604/1/artigo.pdfec3e714cc621660afa9b18022859f40cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/23604/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/236042019-02-20 10:39:29.268oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-02-20T13:39:29LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance |
title |
From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance |
spellingShingle |
From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance Athanassiou, Christos G. Diagnostic indicator Insect movement Mortality Phosphine Red flour beetle Tribolium castaneum Insecticide resistance |
title_short |
From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance |
title_full |
From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance |
title_fullStr |
From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance |
title_full_unstemmed |
From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance |
title_sort |
From immobilization to recovery: Towards the development of a rapid diagnostic indicator for phosphine resistance |
author |
Athanassiou, Christos G. |
author_facet |
Athanassiou, Christos G. Kavallieratos, Nickolas G. Brabec, Daniel L. Oppert, Brenda Campbell, James F. Guedes, Raul N. C. |
author_role |
author |
author2 |
Kavallieratos, Nickolas G. Brabec, Daniel L. Oppert, Brenda Campbell, James F. Guedes, Raul N. C. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Athanassiou, Christos G. Kavallieratos, Nickolas G. Brabec, Daniel L. Oppert, Brenda Campbell, James F. Guedes, Raul N. C. |
dc.subject.pt-BR.fl_str_mv |
Diagnostic indicator Insect movement Mortality Phosphine Red flour beetle Tribolium castaneum Insecticide resistance |
topic |
Diagnostic indicator Insect movement Mortality Phosphine Red flour beetle Tribolium castaneum Insecticide resistance |
description |
The aim of this work was to evaluate the insect mobility patterns of phosphine-resistant and -susceptible adults of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) after exposure to phosphine. Exposure bioassays were carried out at two concentrations, 1000 and 3000 ppm, while adults were observed every 15 min, for a total period of 90 min. During this observation interval, adults were visually classified as active (able to walk normally), partially immobilized (not able to walk, but showing a minimal movement), or completely immobilized (no visible movement). After the observation period, all adults were placed in a phosphine-free environment, and they again were classified as active, partially immobilized or completely immobilized. At 1000 ppm, the majority of adults of the susceptible T. castaneum population were quickly immobilized after a 15 min observation period, while in contrast, the majority of adults of the resistant T. castaneum population were still active after the termination of the 90 min interval. At 3000 ppm, the percentage of immobilized susceptible adults was increased at the 15 min observation period, while the majority of resistant adults were immobilized only after 90 min. In the post-exposure period, the vast majority of the susceptible adults were dead. In contrast, most resistant adults recovered, regardless of the concentration that had been exposed. The results of this study delineate major differences in movement in phosphine-resistant and -susceptible T. castaneum strains, and can be applied as a quick diagnostic bioassay for the evaluation of resistance to phosphine in stored product insects. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-02-20T11:04:03Z |
dc.date.available.fl_str_mv |
2019-02-20T11:04:03Z |
dc.date.issued.fl_str_mv |
2019-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.jspr.2018.10.004 http://www.locus.ufv.br/handle/123456789/23604 |
dc.identifier.issn.none.fl_str_mv |
0022-474X |
identifier_str_mv |
0022-474X |
url |
https://doi.org/10.1016/j.jspr.2018.10.004 http://www.locus.ufv.br/handle/123456789/23604 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 80, Pages 28- 33, January 2019 |
dc.rights.driver.fl_str_mv |
2018 Elsevier Ltd. All rights reserved. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
2018 Elsevier Ltd. All rights reserved. |
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openAccess |
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Journal of Stored Products Research |
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Journal of Stored Products Research |
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