Avaliação do óleo de sacha kiruma (Plukenetia volubilis L.) no duodeno e fígado de camundongos C57BL/6 e APO E-/-

• Autor(a) principal: Thomazini, Bruna Fontana
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2336
• topic: Plukenetia volubilis; Camundongo knockout Apo-E; Ácidos graxos poliinsaturados; Duodeno; Hepatócitos; Plukenetia volubilis; Knockout Apo-E mice; Polyunsaturated fatty acids; Duodenum; Hepatocytes; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Sacha kiruma (Plukenetia volubilis L.) is an oleaginous native plant of the Peruvian Amazon. The oil extracted from its seeds is very rich in polyunsaturated fatty acids, directly related to the prevention of cardiovascular diseases, among them, the atherosclerosis. For transportation, lipids form complexes with proteins, the apoproteins. The apoprotein E (Apo E) is mainly synthesized in the liver and intestine and is related to the cellular uptake of triglyceride-rich chylomicrons and VLDL remmants. The production of knockout animals for the apoprotein E gene presents as main characteristic the rapid and spontaneous development of atherosclerosis lesions in the arteries, similar to those found in humans. The small intestine is the site of absorption of dietary components, including lipids, whiles the liver is the site of modification and/or storage of compounds. The objectives were extract sacha kiruma seeds´s oil by chemical method (hexane) and determine the acid profile. Also evaluate the histological structure of the duodenum and the liver of Apo E-/- mice and wild BlackC57/6 after the treatment with this oil. The evaluation of the oil extracted in this study indicates the percentage of polyunsaturated fatty acids similar to that found in the literature. There were no histological changes due the treatment in the duodenum, with regard to the surface absorption, morphology of the crypt and the thickness of muscle layers and mucosa. With respect to the height of the absorptive epithelium, the control animals have high average compared with animals treated with the oil. The frequency of enterocytes, lymphocytes, goblet cells and Paneth cells did not change due to the proposal treatment. In relation to the goblet cell AB+PAS+ was observed more frequently in the groups of BlackC57/6 mice treated with the sacha kiruma´s oil compared to control groups. In the liver were not found differences due to the treatment in the frequency of liver components or the ratio cytoplasm/ nucleus of the liver cells. The solvent extraction method was efficient in extracting oil from sacha kiruma´s seeds. The oil showed similar lipid composition from the described in the literature. The test showed no evidence that the diet with sacha kiruma oil may have altered the histological structure of the duodenum and liver of mice Apo E -/- or wild BlackC57/6.