Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1111/j.1567-1364.2010.00709.x http://www.locus.ufv.br/handle/123456789/13870 |
Resumo: | Kluyveromyces lactis Lac12 permease mediates lactose and low-affinity galactose transports. In this study we investigated the effects of carbon sources on internalization of Lac12 using a LAC12–GFP fusion construct. When galactose- or lactose-grown cells are shifted to a fresh sugar medium, Lac12–GFP is removed from the plasma membrane and is localized intracellularly. Surprisingly, either galactose or lactose in the new media caused the internalization, and cells responded differently to these two sugars. Our results reveal that this process is dependent on sugar species and also sugar concentration. Lac12–GFP internalization causes reduction of [C14]lactose uptake rates and also occurs in a Klsnf1 mutant strain; it is therefore independent of KlSnf1 activity. We suggest that glucose-6-phosphate is the intracellular signal, as internalization was induced by 2-deoxyglucose, and inhibition of phosphoglucomutase by lithium prevented galactose- but not lactose- or glucose-induced internalization. Lac12–GFP internalization was not triggered by 6-deoxyglucose, and was irreversible in the absence of protein synthesis. |
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Rigamonte, Tatiana A.Silveira, Wendel B.Fietto, Luciano G.Castro, Ieso M.Breunig, Karin D.Passos, Flavia M.L.2017-11-28T10:50:29Z2017-11-28T10:50:29Z2011-01-1915671364https://doi.org/10.1111/j.1567-1364.2010.00709.xhttp://www.locus.ufv.br/handle/123456789/13870Kluyveromyces lactis Lac12 permease mediates lactose and low-affinity galactose transports. In this study we investigated the effects of carbon sources on internalization of Lac12 using a LAC12–GFP fusion construct. When galactose- or lactose-grown cells are shifted to a fresh sugar medium, Lac12–GFP is removed from the plasma membrane and is localized intracellularly. Surprisingly, either galactose or lactose in the new media caused the internalization, and cells responded differently to these two sugars. Our results reveal that this process is dependent on sugar species and also sugar concentration. Lac12–GFP internalization causes reduction of [C14]lactose uptake rates and also occurs in a Klsnf1 mutant strain; it is therefore independent of KlSnf1 activity. We suggest that glucose-6-phosphate is the intracellular signal, as internalization was induced by 2-deoxyglucose, and inhibition of phosphoglucomutase by lithium prevented galactose- but not lactose- or glucose-induced internalization. Lac12–GFP internalization was not triggered by 6-deoxyglucose, and was irreversible in the absence of protein synthesis.engFEMS Yeast ResearchVolume 11, Issue 3, Pages 243–251, May 2011Kluyveromyces lactisLactose permeaseLac12Post-translational controlCatabolite inactivationSugar transportRestricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALwatermark.pdfwatermark.pdfTexto completoapplication/pdf483301https://locus.ufv.br//bitstream/123456789/13870/1/watermark.pdf848a9f88c5c3916fac87438de650e75dMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/13870/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILwatermark.pdf.jpgwatermark.pdf.jpgIM Thumbnailimage/jpeg5346https://locus.ufv.br//bitstream/123456789/13870/3/watermark.pdf.jpg3b2c8f9cbad51fd666d2a7b3a91b118dMD53123456789/138702017-11-28 22:00:58.065oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-29T01:00:58LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12 |
title |
Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12 |
spellingShingle |
Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12 Rigamonte, Tatiana A. Kluyveromyces lactis Lactose permease Lac12 Post-translational control Catabolite inactivation Sugar transport |
title_short |
Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12 |
title_full |
Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12 |
title_fullStr |
Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12 |
title_full_unstemmed |
Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12 |
title_sort |
Restricted sugar uptake by sugar-induced internalization of the yeast lactose/galactose permease Lac12 |
author |
Rigamonte, Tatiana A. |
author_facet |
Rigamonte, Tatiana A. Silveira, Wendel B. Fietto, Luciano G. Castro, Ieso M. Breunig, Karin D. Passos, Flavia M.L. |
author_role |
author |
author2 |
Silveira, Wendel B. Fietto, Luciano G. Castro, Ieso M. Breunig, Karin D. Passos, Flavia M.L. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rigamonte, Tatiana A. Silveira, Wendel B. Fietto, Luciano G. Castro, Ieso M. Breunig, Karin D. Passos, Flavia M.L. |
dc.subject.pt-BR.fl_str_mv |
Kluyveromyces lactis Lactose permease Lac12 Post-translational control Catabolite inactivation Sugar transport |
topic |
Kluyveromyces lactis Lactose permease Lac12 Post-translational control Catabolite inactivation Sugar transport |
description |
Kluyveromyces lactis Lac12 permease mediates lactose and low-affinity galactose transports. In this study we investigated the effects of carbon sources on internalization of Lac12 using a LAC12–GFP fusion construct. When galactose- or lactose-grown cells are shifted to a fresh sugar medium, Lac12–GFP is removed from the plasma membrane and is localized intracellularly. Surprisingly, either galactose or lactose in the new media caused the internalization, and cells responded differently to these two sugars. Our results reveal that this process is dependent on sugar species and also sugar concentration. Lac12–GFP internalization causes reduction of [C14]lactose uptake rates and also occurs in a Klsnf1 mutant strain; it is therefore independent of KlSnf1 activity. We suggest that glucose-6-phosphate is the intracellular signal, as internalization was induced by 2-deoxyglucose, and inhibition of phosphoglucomutase by lithium prevented galactose- but not lactose- or glucose-induced internalization. Lac12–GFP internalization was not triggered by 6-deoxyglucose, and was irreversible in the absence of protein synthesis. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-01-19 |
dc.date.accessioned.fl_str_mv |
2017-11-28T10:50:29Z |
dc.date.available.fl_str_mv |
2017-11-28T10:50:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1111/j.1567-1364.2010.00709.x http://www.locus.ufv.br/handle/123456789/13870 |
dc.identifier.issn.none.fl_str_mv |
15671364 |
identifier_str_mv |
15671364 |
url |
https://doi.org/10.1111/j.1567-1364.2010.00709.x http://www.locus.ufv.br/handle/123456789/13870 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 11, Issue 3, Pages 243–251, May 2011 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
FEMS Yeast Research |
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FEMS Yeast Research |
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