Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/4349 |
Resumo: | The influence of different concentrations of As over some aspects of growth and antioxidant system were studied in three water species: Azolla caroliniana, Lemna gibba and Salvinia minima, in nutrient solution. After the three species being exposed to five concentrations of As (0.0, 0.25, 0.5, 1.0 and 1.5 mg.L-1) in nutrient solution, the As content into the plants, relative growth rate (TCR), superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutatione reductase (GR) enzymatic activity, total thiol, non protein thiol and protein thiol content and anthocyanin content were determined. L. gibba showed the highest tolerance to As, requiring 967,6 μg g-1MD to reduce growth in 50%. A. caroliniana required 429,2 μg g-1MD to show this level of growth reduction and S. minima required 255,08 μg g-1MD for the same response. Antioxidant mechanisms to the increase in As concentration in the medium characterized each species. A. caroliniana went through increases in CAT and GR activities, keeping unaltered SOD and POX activities, and showing reduction in APX activity, although it presented the highest activity of this enzyme. S. minima exhibited increase in SOD, CAT, APX and GR activities, keeping POX activity unaltered, with the increase of As concentration in the growth medium. L. gibba higher tolerance to As effects seem to be related to the enzymatic antioxidant mechanisms and also to the non-enzymatic ones. This species, besides presenting higher SOD, CAT, POX and GR activities, also showed higher thiol and anthocyanin content than the other two species studied. Although A. caroliniana and S. minima also presented increase in the activity of several enzymes, L. gibba s higher tolerance seems to be due not only to the increase in these antioxidant processes, but also to superior basal activity of these enzymes, and to higher thiol and anthocyanin basal content. Besides the increase in enzymatic activities, L. gibba exhibited total-thiol and non-protein thiol content increase, diminishing protein thiol content. A. caroliniana and S. minima practically did not suffer alterations in thiol content. As L. gibba, A. caroliniana and S. minima revealed an increment in anthocyanin content after exposure to increasing concentrations of As in the solution. In this context, L. gibba showed the highest potential to be employed in phytoremediation. |
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Santos, Gabriela Alves doshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4718438Y2Silva, Marco Aurélio Pedron ehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790898P8Cambraia, Joséhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783868U6Oliveira, Juraci Alves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782512D8Euclydes, Rosane Maria de Aguiarhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786094T9Ribeiro, Sylvia Therese Meyerhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780180A02015-03-26T13:36:46Z2007-07-192015-03-26T13:36:46Z2006-09-29SANTOS, Gabriela Alves dos. Growth and antioxidative responses of aquatic macrophytes to arsenic. 2006. 55 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2006.http://locus.ufv.br/handle/123456789/4349The influence of different concentrations of As over some aspects of growth and antioxidant system were studied in three water species: Azolla caroliniana, Lemna gibba and Salvinia minima, in nutrient solution. After the three species being exposed to five concentrations of As (0.0, 0.25, 0.5, 1.0 and 1.5 mg.L-1) in nutrient solution, the As content into the plants, relative growth rate (TCR), superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutatione reductase (GR) enzymatic activity, total thiol, non protein thiol and protein thiol content and anthocyanin content were determined. L. gibba showed the highest tolerance to As, requiring 967,6 μg g-1MD to reduce growth in 50%. A. caroliniana required 429,2 μg g-1MD to show this level of growth reduction and S. minima required 255,08 μg g-1MD for the same response. Antioxidant mechanisms to the increase in As concentration in the medium characterized each species. A. caroliniana went through increases in CAT and GR activities, keeping unaltered SOD and POX activities, and showing reduction in APX activity, although it presented the highest activity of this enzyme. S. minima exhibited increase in SOD, CAT, APX and GR activities, keeping POX activity unaltered, with the increase of As concentration in the growth medium. L. gibba higher tolerance to As effects seem to be related to the enzymatic antioxidant mechanisms and also to the non-enzymatic ones. This species, besides presenting higher SOD, CAT, POX and GR activities, also showed higher thiol and anthocyanin content than the other two species studied. Although A. caroliniana and S. minima also presented increase in the activity of several enzymes, L. gibba s higher tolerance seems to be due not only to the increase in these antioxidant processes, but also to superior basal activity of these enzymes, and to higher thiol and anthocyanin basal content. Besides the increase in enzymatic activities, L. gibba exhibited total-thiol and non-protein thiol content increase, diminishing protein thiol content. A. caroliniana and S. minima practically did not suffer alterations in thiol content. As L. gibba, A. caroliniana and S. minima revealed an increment in anthocyanin content after exposure to increasing concentrations of As in the solution. In this context, L. gibba showed the highest potential to be employed in phytoremediation.A influência do As sobre alguns aspectos do crescimento e do sistema antioxidante foi estudada em três espécies aquáticas: Azolla caroliniana, Lemna gibba e Salvinia minima, expostas cinco concentrações de As (0,0, 0,25, 0,5, 1,0 e 1,5 mg L-1) em solução nutritiva. Foram determinados o teor de As nas plantas, a taxa de crescimento relativo (TCR), as atividades enzimáticas da dismutase do superóxido (SOD), da catalase (CAT), de peroxidases (POX), da peroxidase do ascorbato (APX) e da redutase da glutationa (GR), o teor de tióis totais, de tióis não protéicos e de tióis protéicos e, ainda, o teor de antocianinas. Lemna gibba foi a macrófita que apresentou maior tolerância ao As, requerendo 967,6 μg g-1MS para reduzir em 50 % a TCR. Azolla caroliniana demandou 429,2 μg g-1MS para a ocorrência dessa redução e, por último, demonstrando ser a mais sensível, S. minima requereu 255,08 μg g-1MS. Em relação aos mecanismos antioxidantes, as três espécies responderam de forma diferente ao aumento da concentração de As no meio. Azolla caroliniana sofreu aumentos nas atividades da CAT e da GR, manteve inalteradas as atividades da SOD e da POX, sofrendo diminuição na atividade da APX, embora tenha sido a espécie que apresentou maior atividade desta enzima. Salvinia minima exibiu aumentos nas atividades de SOD, CAT, APX e GR, mantendo inalterada a atividade de POX, com o aumento da concentração de As no meio de cultivo. A maior tolerância de L. gibba aos efeitos do As parece estar relacionada tanto aos mecanismos antioxidantes enzimáticos e não enzimáticos. Esta espécie, além de apresentar maiores atividades de SOD, CAT, POX e GR, também apresentou maiores teores de tióis e antocianinas que as outras duas espécies analisadas. Embora A. caroliniana e S. minima também tenham apresentado incrementos nas atividades de várias enzimas, a maior tolerância de L. gibba parece ser devida não somente ao aumento nestes processos antioxidantes, mas à superior atividade basal dessas enzimas e ao maior conteúdo basal de tióis e antocianinas. Além do aumento nas atividades enzimáticas, L. gibba exibiu aumentos nos teores de tióis totais e tióis não protéicos, diminuindo os teores de tióis protéicos. A. caroliniana e S. minima praticamente não sofreram alterações no conteúdo de tióis. Assim como L. gibba, A. caroliniana e S. minima revelaram acréscimos nos teores de antocianinas após exposição às concentrações crescentes de As na solução. Neste contexto, dentre as três macrófitas estudadas, L. gibba foi a que apresentou maior potencial para ser empregada em programas de fitorremediação.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Fisiologia VegetalUFVBRControle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superioresLemnaSalviniaAzollaFitorremediaçãoLemnaSalviniaAzollaPhytoremediationCNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETALCrescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênioGrowth and antioxidative responses of aquatic macrophytes to arsenicinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf279034https://locus.ufv.br//bitstream/123456789/4349/1/texto%20completo.pdf141cff7d975ad2a0d439e0f2d5e92fd3MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain102132https://locus.ufv.br//bitstream/123456789/4349/2/texto%20completo.pdf.txt63248298c9e64356ceb6fe5fea764f72MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3707https://locus.ufv.br//bitstream/123456789/4349/3/texto%20completo.pdf.jpgcbcfa8d51668ca8f559e05b5b3fcbb74MD53123456789/43492016-04-10 23:06:39.874oai:locus.ufv.br:123456789/4349Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:06:39LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio |
dc.title.alternative.eng.fl_str_mv |
Growth and antioxidative responses of aquatic macrophytes to arsenic |
title |
Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio |
spellingShingle |
Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio Santos, Gabriela Alves dos Lemna Salvinia Azolla Fitorremediação Lemna Salvinia Azolla Phytoremediation CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio |
title_full |
Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio |
title_fullStr |
Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio |
title_full_unstemmed |
Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio |
title_sort |
Crescimento e respostas antioxidantes de macrófitas aquáticas submetidas ao arsênio |
author |
Santos, Gabriela Alves dos |
author_facet |
Santos, Gabriela Alves dos |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4718438Y2 |
dc.contributor.author.fl_str_mv |
Santos, Gabriela Alves dos |
dc.contributor.advisor-co1.fl_str_mv |
Silva, Marco Aurélio Pedron e |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790898P8 |
dc.contributor.advisor-co2.fl_str_mv |
Cambraia, José |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783868U6 |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Juraci Alves de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782512D8 |
dc.contributor.referee1.fl_str_mv |
Euclydes, Rosane Maria de Aguiar |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786094T9 |
dc.contributor.referee2.fl_str_mv |
Ribeiro, Sylvia Therese Meyer |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780180A0 |
contributor_str_mv |
Silva, Marco Aurélio Pedron e Cambraia, José Oliveira, Juraci Alves de Euclydes, Rosane Maria de Aguiar Ribeiro, Sylvia Therese Meyer |
dc.subject.por.fl_str_mv |
Lemna Salvinia Azolla Fitorremediação |
topic |
Lemna Salvinia Azolla Fitorremediação Lemna Salvinia Azolla Phytoremediation CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.eng.fl_str_mv |
Lemna Salvinia Azolla Phytoremediation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
description |
The influence of different concentrations of As over some aspects of growth and antioxidant system were studied in three water species: Azolla caroliniana, Lemna gibba and Salvinia minima, in nutrient solution. After the three species being exposed to five concentrations of As (0.0, 0.25, 0.5, 1.0 and 1.5 mg.L-1) in nutrient solution, the As content into the plants, relative growth rate (TCR), superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutatione reductase (GR) enzymatic activity, total thiol, non protein thiol and protein thiol content and anthocyanin content were determined. L. gibba showed the highest tolerance to As, requiring 967,6 μg g-1MD to reduce growth in 50%. A. caroliniana required 429,2 μg g-1MD to show this level of growth reduction and S. minima required 255,08 μg g-1MD for the same response. Antioxidant mechanisms to the increase in As concentration in the medium characterized each species. A. caroliniana went through increases in CAT and GR activities, keeping unaltered SOD and POX activities, and showing reduction in APX activity, although it presented the highest activity of this enzyme. S. minima exhibited increase in SOD, CAT, APX and GR activities, keeping POX activity unaltered, with the increase of As concentration in the growth medium. L. gibba higher tolerance to As effects seem to be related to the enzymatic antioxidant mechanisms and also to the non-enzymatic ones. This species, besides presenting higher SOD, CAT, POX and GR activities, also showed higher thiol and anthocyanin content than the other two species studied. Although A. caroliniana and S. minima also presented increase in the activity of several enzymes, L. gibba s higher tolerance seems to be due not only to the increase in these antioxidant processes, but also to superior basal activity of these enzymes, and to higher thiol and anthocyanin basal content. Besides the increase in enzymatic activities, L. gibba exhibited total-thiol and non-protein thiol content increase, diminishing protein thiol content. A. caroliniana and S. minima practically did not suffer alterations in thiol content. As L. gibba, A. caroliniana and S. minima revealed an increment in anthocyanin content after exposure to increasing concentrations of As in the solution. In this context, L. gibba showed the highest potential to be employed in phytoremediation. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-09-29 |
dc.date.available.fl_str_mv |
2007-07-19 2015-03-26T13:36:46Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:36:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Gabriela Alves dos. Growth and antioxidative responses of aquatic macrophytes to arsenic. 2006. 55 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2006. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/4349 |
identifier_str_mv |
SANTOS, Gabriela Alves dos. Growth and antioxidative responses of aquatic macrophytes to arsenic. 2006. 55 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2006. |
url |
http://locus.ufv.br/handle/123456789/4349 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Mestrado em Fisiologia Vegetal |
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UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores |
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Universidade Federal de Viçosa |
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