Adição de BSA ao diluente BTS modificado na criopreservação de sêmen caprino

• Autor(a) principal: Oliveira, Carlos Thiago Silveira Alvim Mendes de
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5735
• topic: Albumina; Criopreservação; Sêmen caprino; Albumin; Cryopreservation; Goat semen; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL

The objectives of this study were to examine whether albumin serum bovine has an effect on the structural function of goat sperm plasma membrane, as well to investigate the potential use of this protein in extenders medium for cryopreservation.Two adult Alpina breed males and two adult Saanen breed males are used, totalizing five semen samples for each one. After collection, the physical characteristics of the semen were evaluated. Then the semen was diluted as follows: BIOXCELL® (Control - T1), BTS (T2), BTS + BSA 5% (T3) and BTS + BSA 15% (T4). After the final dilutions, the progressive motility and sperm vigor were performed for each treatment. The semen was packaged in 025 ml straws were cooled to five degrees for one hour in plastic container containing ethyl alcohol. The pre-freezing was done in liquid nitrogen vapor for 15 minutes. After this period, the straws were immersed in liquid nitrogen. The samples were thawed in a water bath at 37 °C for 30 seconds, packaged in micro tubes and homogenized for immediate analysis of sperm motility and vigor, hypoosmotic swelling test (HOST) and thermoresistence test. In fresh semen, the physical characteristics remained within normal parameters. The average volume correlated negatively with sperm concentration and progressive motility (r = - 0,38 and 0,42 respectively). A positive correlation (r = 0,60) was found between fresh sperm motile and turbulence. The overall averages of motility did not differ among treatments, after thawing at zero and 30 minutes, but differed over the TTR (P < 0.05).The average vigor did not differ in time 30 minutes, but differed between treatments in other times (P<0.05). The strength of the thawed, the control group, showed a negative correlation with the vigor of fresh semen and positive correlation with the motility of frozen-thawed semen (r = - 0.38 and 0.54 respectively). The functional integrity of sperm membrane, evaluated by hypoosmotic swelling test, was different between the control group and the other treatments (P<0.05). There was negative correlation (r = - 0.55) between the turbulence and the HOST of treatment two. In treatment three, positive correlation found between concentration and HOST. It is concluded that: The albumin serum bovine associated with the modified did not affect any of parameters under study.