Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil

Detalhes bibliográficos
Autor(a) principal: Mafra, Cláudio
Data de Publicação: 2015
Outros Autores: Mantovani, Cynthia, Borges, Juliana Novo, Barcelos, Rafael Mazioli, Santos, Cláudia Portes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1984-29612015037
http://www.locus.ufv.br/handle/123456789/12441
Resumo: Uma larva de Nematoda anisakídeo encontrada em bacalhau comercializado no Estado de Minas Gerais, Brasil foi estudada por microscopias de luz e eletrônica de varredura e por uma abordagem molecular. As regiões da subunidade 2 da citocromo c-oxidase mitocondrial (mtDNA cox-2), 28S rRNA e ITS1, 5.8S e ITS2 foram amplificadas usando a reação em cadeia da polimerase e sequenciadas para avaliar as relações filogenéticas da larva. O perfil genético confirmou que esta larva pertence à espécie Pseudoterranova decipiens (sensu stricto). Esse é o primeiro estudo molecular e ultraestrutural de Pseudoterranova decipiens (sensu stricto) de bacalhau importado vendido no Brasil. As implicações destes resultados para a saúde são discutidas.
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spelling Mafra, CláudioMantovani, CynthiaBorges, Juliana NovoBarcelos, Rafael MazioliSantos, Cláudia Portes2017-10-26T13:09:56Z2017-10-26T13:09:56Z2015-04-231984-2961http://dx.doi.org/10.1590/S1984-29612015037http://www.locus.ufv.br/handle/123456789/12441Uma larva de Nematoda anisakídeo encontrada em bacalhau comercializado no Estado de Minas Gerais, Brasil foi estudada por microscopias de luz e eletrônica de varredura e por uma abordagem molecular. As regiões da subunidade 2 da citocromo c-oxidase mitocondrial (mtDNA cox-2), 28S rRNA e ITS1, 5.8S e ITS2 foram amplificadas usando a reação em cadeia da polimerase e sequenciadas para avaliar as relações filogenéticas da larva. O perfil genético confirmou que esta larva pertence à espécie Pseudoterranova decipiens (sensu stricto). Esse é o primeiro estudo molecular e ultraestrutural de Pseudoterranova decipiens (sensu stricto) de bacalhau importado vendido no Brasil. As implicações destes resultados para a saúde são discutidas.An anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), 28S rRNA and ITS1, 5.8S and ITS2 regions were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships of the larva. The genetic profile confirmed that this larva belongs to the species Pseudoterranova decipiens (sensu stricto). This is the first molecular and ultrastructural study of Pseudoterranova decipiens (sensu stricto) in imported cod sold in Brazil. The health implications of these findings are discussed.engRevista Brasileira de Parasitologia Veterináriav. 24, n. 2, p. 209-215, abr.-jun. 2015AnisakiasisCodwormFood safetyPublic healthMorphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL1984-2961-rbpv-24-2-209.pdf1984-2961-rbpv-24-2-209.pdftexto completoapplication/pdf1346423https://locus.ufv.br//bitstream/123456789/12441/1/1984-2961-rbpv-24-2-209.pdfa2752fb9d477f3ddb4c5dece91333de6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12441/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAIL1984-2961-rbpv-24-2-209.pdf.jpg1984-2961-rbpv-24-2-209.pdf.jpgIM Thumbnailimage/jpeg4456https://locus.ufv.br//bitstream/123456789/12441/3/1984-2961-rbpv-24-2-209.pdf.jpg0321b18ce4e7c21593807412c5fbec2dMD53123456789/124412017-10-26 22:01:02.734oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-27T01:01:02LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
title Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
spellingShingle Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
Mafra, Cláudio
Anisakiasis
Codworm
Food safety
Public health
title_short Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
title_full Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
title_fullStr Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
title_full_unstemmed Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
title_sort Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
author Mafra, Cláudio
author_facet Mafra, Cláudio
Mantovani, Cynthia
Borges, Juliana Novo
Barcelos, Rafael Mazioli
Santos, Cláudia Portes
author_role author
author2 Mantovani, Cynthia
Borges, Juliana Novo
Barcelos, Rafael Mazioli
Santos, Cláudia Portes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mafra, Cláudio
Mantovani, Cynthia
Borges, Juliana Novo
Barcelos, Rafael Mazioli
Santos, Cláudia Portes
dc.subject.pt-BR.fl_str_mv Anisakiasis
Codworm
Food safety
Public health
topic Anisakiasis
Codworm
Food safety
Public health
description Uma larva de Nematoda anisakídeo encontrada em bacalhau comercializado no Estado de Minas Gerais, Brasil foi estudada por microscopias de luz e eletrônica de varredura e por uma abordagem molecular. As regiões da subunidade 2 da citocromo c-oxidase mitocondrial (mtDNA cox-2), 28S rRNA e ITS1, 5.8S e ITS2 foram amplificadas usando a reação em cadeia da polimerase e sequenciadas para avaliar as relações filogenéticas da larva. O perfil genético confirmou que esta larva pertence à espécie Pseudoterranova decipiens (sensu stricto). Esse é o primeiro estudo molecular e ultraestrutural de Pseudoterranova decipiens (sensu stricto) de bacalhau importado vendido no Brasil. As implicações destes resultados para a saúde são discutidas.
publishDate 2015
dc.date.issued.fl_str_mv 2015-04-23
dc.date.accessioned.fl_str_mv 2017-10-26T13:09:56Z
dc.date.available.fl_str_mv 2017-10-26T13:09:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1984-29612015037
http://www.locus.ufv.br/handle/123456789/12441
dc.identifier.issn.none.fl_str_mv 1984-2961
identifier_str_mv 1984-2961
url http://dx.doi.org/10.1590/S1984-29612015037
http://www.locus.ufv.br/handle/123456789/12441
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 24, n. 2, p. 209-215, abr.-jun. 2015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária
publisher.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
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