Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)

Detalhes bibliográficos
Autor(a) principal: Castrillon, Luis Carlos Martinez
Data de Publicação: 2014
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/934
Resumo: Podisus nigrispinus Dallas (Hemiptera: Pentatomidae) is a zoophytophagous insect that has a potential in biological control because its nymphs and adults prey on various insects. They insert their mouthparts and inject the contents of their salivary glands in their prey to kill them. Although it is understood that the saliva of this predator is toxic, the toxic compounds responsible for the prey death remains unknown. To identify the components of the saliva of P. nigrispinus, we evaluated the ultrastructure and cytochemistry of the salivary glands of this insect. Cytochemistry evaluation identified the compounds of the saliva of P. nigrispinus responsible for the death of Anticarsia gemmatalis prey. The salivary system of P. nigrispinus has consists of a pair of principal salivary glands, which are bilobed with a short anterior and a long posterior lobe, and a pair of tubular accessory glands. The cells of both the glands are well developed with a predominance of mitochondria and rough endoplasmic reticulum and with basal plasma membrane infoldings, indicating that both the glands play a role in the transport of hemolymph substances and protein synthesis. The cytochemical tests demonstrated positive reactions for carbohydrate, protein, and acid phosphatase in the cells of both the salivary glands. An injection of the saliva of P. nigrispinus exposed to enzyme inhibitors and proteases and extracted at the ether phase caused mortality of A. gemmatalis larvae, with LD50 = 1.91 and LD90 = 4.34 μL. These saliva fractions were identified as N,N-dimethylaniline and 1,2,3-trithiepane, and the injection of the first compound showed an LC50 = 136.1 nL and LC90 = 413.8 nL, suggesting that these non- proteineous compound is responsible for the saliva toxicity of P. nigrispinus. The ultrastructural and cytochemical features suggested participation of the principal and accessory salivary glands in the secretion of proteinaceous and non-proteinaceous toxic substances in the saliva of this insect. This was further confirmed by identification of N,N-dimethylaniline and 1,2,3-trithiepane responsible for the death of A. gemmatalis. This is the first study reporting the toxic compounds of the saliva of this insect.
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spelling Castrillon, Luis Carlos Martinezhttp://lattes.cnpq.br/3273371644219437Zanuncio, José Colahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787556T2Fialho, Maria do Carmo Queirozhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4127872D4Serrão, José Eduardohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785636U6Alves, Stênio Nuneshttp://lattes.cnpq.br/8281387424304808Lemos, Walkymário de Paulohttp://lattes.cnpq.br/6841621785311887Oliveira, Leandro Licursi dehttp://lattes.cnpq.br/0578231392218162Oliveira, Eugenio Eduardo dehttp://lattes.cnpq.br/00985640669360242015-03-26T12:36:05Z2015-01-262015-03-26T12:36:05Z2014-05-23CASTRILLON, Luis Carlos Martinez. Ultrastructure and function of the salivary glands of Podisus nigrispinus (Hemiptera: Pentatomidae). 2014. 47 f. Tese (Doutorado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/934Podisus nigrispinus Dallas (Hemiptera: Pentatomidae) is a zoophytophagous insect that has a potential in biological control because its nymphs and adults prey on various insects. They insert their mouthparts and inject the contents of their salivary glands in their prey to kill them. Although it is understood that the saliva of this predator is toxic, the toxic compounds responsible for the prey death remains unknown. To identify the components of the saliva of P. nigrispinus, we evaluated the ultrastructure and cytochemistry of the salivary glands of this insect. Cytochemistry evaluation identified the compounds of the saliva of P. nigrispinus responsible for the death of Anticarsia gemmatalis prey. The salivary system of P. nigrispinus has consists of a pair of principal salivary glands, which are bilobed with a short anterior and a long posterior lobe, and a pair of tubular accessory glands. The cells of both the glands are well developed with a predominance of mitochondria and rough endoplasmic reticulum and with basal plasma membrane infoldings, indicating that both the glands play a role in the transport of hemolymph substances and protein synthesis. The cytochemical tests demonstrated positive reactions for carbohydrate, protein, and acid phosphatase in the cells of both the salivary glands. An injection of the saliva of P. nigrispinus exposed to enzyme inhibitors and proteases and extracted at the ether phase caused mortality of A. gemmatalis larvae, with LD50 = 1.91 and LD90 = 4.34 μL. These saliva fractions were identified as N,N-dimethylaniline and 1,2,3-trithiepane, and the injection of the first compound showed an LC50 = 136.1 nL and LC90 = 413.8 nL, suggesting that these non- proteineous compound is responsible for the saliva toxicity of P. nigrispinus. The ultrastructural and cytochemical features suggested participation of the principal and accessory salivary glands in the secretion of proteinaceous and non-proteinaceous toxic substances in the saliva of this insect. This was further confirmed by identification of N,N-dimethylaniline and 1,2,3-trithiepane responsible for the death of A. gemmatalis. This is the first study reporting the toxic compounds of the saliva of this insect.Podisus nigrispinus Dallas (Hemiptera: Pentatomidae) é um inseto zoofitófago com potencial para controle biológico, pois ninfas e adultos predam diversos insetos, inserindo o aparelho bucal e injetando o conteúdo das glândulas salivares no interior das presas, causando a morte das mesmas. Entretanto, os compostos tóxicos da saliva desse inseto, responsáveis pela morte das presas são ainda desconhecidos. Como primeira etapa para a identificação das possíveis substâncias presentes na saliva de P. nigrispinus, este estudo avaliou a ultraestrutura e citoquímica das glândulas salivares de P. nigrispinus. A segunda etapa identificou os compostos da saliva de P. nigrispinus responsáveis pela morte da presa Anticarsia gemmatalis. A avaliação da ultraestrututra das glândulas salivares mostrou que o sistema salivar de P. nigrispinus é composto por um par de glândulas salivares principais bilobadas, formando um lobo anterior curto e um posterior longo e um par de glândulas acessórias tubulares. As células de ambas as glândulas foram bem desenvolvidas com predomínio de mitocôndrias e retículo endoplasmático rugoso, além de invaginações da membrana plasmática basal, indicando que ambas as glândulas tem função de transporte de substâncias a partir da hemolinfa bem como na síntese de proteínas. Os testes citoquímicos mostraram reações positivas no citoplasma para carboidratos, fosfatase ácida e proteínas nas diferentes regiões do sistema glandular. A saliva de P. nigrispinus exposta a inibidores enzimáticos, proteases e na fase de éter causou mortalidade após injeção em lagartas de A. gemmatalis, sendo a CL50 de 1,91 e CL90 de 4,34 μL do extrato em éter de saliva. Frações do extrato salivar em éter que causaram mortalidade de A. gemmatalis correspondem aos compostos N,N- dimetil anilina e 1,2,3-tritiepano, sendo que a injeção do primeiro composto apresentou CL50 de 136,1 nL e CL90 de 413,8 nL, sugerindo que este composto não proteico da saliva é um dos responsáveis pela toxicidade da saliva de P. nigrispinus. As características ultraestruturais e citoquímicas sugeriram que as glândulas salivares principais e acessórias participam da secreção de proteínas e substâncias tóxicas não proteicas na saliva desse inseto. Isto foi corroborado pela identificação da N,N-dimetil anilina e o 1,2,3-tritiepano responsáveis pela morte da A. gemmatalis os quais são reportados pela primeira vez na saliva de insetos.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em EntomologiaUFVBRCiência entomológica; Tecnologia entomológicaPodisus nigrispinus - Controle biológicoGlândulas salivaresEnzimasN,N-dimetilanilina1,2,3-tritiepanoPodisus - Biological controlSalivary glandsenzymesN, N-dimethylaniline1,2,3-tritiepanoCNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA::FISIOLOGIA DOS GRUPOS RECENTESUltraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)Ultrastructure and function of the salivary glands of Podisus nigrispinus (Hemiptera: Pentatomidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf3142247https://locus.ufv.br//bitstream/123456789/934/1/texto%20completo.pdf9d3814dbdf939e5c4776cce79113de36MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain66042https://locus.ufv.br//bitstream/123456789/934/2/texto%20completo.pdf.txt99464e0869b41d5b4984d257b50b7204MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3611https://locus.ufv.br//bitstream/123456789/934/3/texto%20completo.pdf.jpg486d029d2a47b36a2382bb03f5d95fe3MD53123456789/9342016-04-06 23:18:24.126oai:locus.ufv.br:123456789/934Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:18:24LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)
dc.title.alternative.eng.fl_str_mv Ultrastructure and function of the salivary glands of Podisus nigrispinus (Hemiptera: Pentatomidae)
title Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)
spellingShingle Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)
Castrillon, Luis Carlos Martinez
Podisus nigrispinus - Controle biológico
Glândulas salivares
Enzimas
N,N-dimetilanilina
1,2,3-tritiepano
Podisus - Biological control
Salivary glands
enzymes
N, N-dimethylaniline
1,2,3-tritiepano
CNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA::FISIOLOGIA DOS GRUPOS RECENTES
title_short Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)
title_full Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)
title_fullStr Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)
title_full_unstemmed Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)
title_sort Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)
author Castrillon, Luis Carlos Martinez
author_facet Castrillon, Luis Carlos Martinez
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/3273371644219437
dc.contributor.author.fl_str_mv Castrillon, Luis Carlos Martinez
dc.contributor.advisor-co1.fl_str_mv Zanuncio, José Cola
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787556T2
dc.contributor.advisor-co2.fl_str_mv Fialho, Maria do Carmo Queiroz
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4127872D4
dc.contributor.advisor1.fl_str_mv Serrão, José Eduardo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785636U6
dc.contributor.referee1.fl_str_mv Alves, Stênio Nunes
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/8281387424304808
dc.contributor.referee2.fl_str_mv Lemos, Walkymário de Paulo
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/6841621785311887
dc.contributor.referee3.fl_str_mv Oliveira, Leandro Licursi de
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/0578231392218162
dc.contributor.referee4.fl_str_mv Oliveira, Eugenio Eduardo de
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/0098564066936024
contributor_str_mv Zanuncio, José Cola
Fialho, Maria do Carmo Queiroz
Serrão, José Eduardo
Alves, Stênio Nunes
Lemos, Walkymário de Paulo
Oliveira, Leandro Licursi de
Oliveira, Eugenio Eduardo de
dc.subject.por.fl_str_mv Podisus nigrispinus - Controle biológico
Glândulas salivares
Enzimas
N,N-dimetilanilina
1,2,3-tritiepano
topic Podisus nigrispinus - Controle biológico
Glândulas salivares
Enzimas
N,N-dimetilanilina
1,2,3-tritiepano
Podisus - Biological control
Salivary glands
enzymes
N, N-dimethylaniline
1,2,3-tritiepano
CNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA::FISIOLOGIA DOS GRUPOS RECENTES
dc.subject.eng.fl_str_mv Podisus - Biological control
Salivary glands
enzymes
N, N-dimethylaniline
1,2,3-tritiepano
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA::FISIOLOGIA DOS GRUPOS RECENTES
description Podisus nigrispinus Dallas (Hemiptera: Pentatomidae) is a zoophytophagous insect that has a potential in biological control because its nymphs and adults prey on various insects. They insert their mouthparts and inject the contents of their salivary glands in their prey to kill them. Although it is understood that the saliva of this predator is toxic, the toxic compounds responsible for the prey death remains unknown. To identify the components of the saliva of P. nigrispinus, we evaluated the ultrastructure and cytochemistry of the salivary glands of this insect. Cytochemistry evaluation identified the compounds of the saliva of P. nigrispinus responsible for the death of Anticarsia gemmatalis prey. The salivary system of P. nigrispinus has consists of a pair of principal salivary glands, which are bilobed with a short anterior and a long posterior lobe, and a pair of tubular accessory glands. The cells of both the glands are well developed with a predominance of mitochondria and rough endoplasmic reticulum and with basal plasma membrane infoldings, indicating that both the glands play a role in the transport of hemolymph substances and protein synthesis. The cytochemical tests demonstrated positive reactions for carbohydrate, protein, and acid phosphatase in the cells of both the salivary glands. An injection of the saliva of P. nigrispinus exposed to enzyme inhibitors and proteases and extracted at the ether phase caused mortality of A. gemmatalis larvae, with LD50 = 1.91 and LD90 = 4.34 μL. These saliva fractions were identified as N,N-dimethylaniline and 1,2,3-trithiepane, and the injection of the first compound showed an LC50 = 136.1 nL and LC90 = 413.8 nL, suggesting that these non- proteineous compound is responsible for the saliva toxicity of P. nigrispinus. The ultrastructural and cytochemical features suggested participation of the principal and accessory salivary glands in the secretion of proteinaceous and non-proteinaceous toxic substances in the saliva of this insect. This was further confirmed by identification of N,N-dimethylaniline and 1,2,3-trithiepane responsible for the death of A. gemmatalis. This is the first study reporting the toxic compounds of the saliva of this insect.
publishDate 2014
dc.date.issued.fl_str_mv 2014-05-23
dc.date.accessioned.fl_str_mv 2015-03-26T12:36:05Z
dc.date.available.fl_str_mv 2015-01-26
2015-03-26T12:36:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv CASTRILLON, Luis Carlos Martinez. Ultrastructure and function of the salivary glands of Podisus nigrispinus (Hemiptera: Pentatomidae). 2014. 47 f. Tese (Doutorado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2014.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/934
identifier_str_mv CASTRILLON, Luis Carlos Martinez. Ultrastructure and function of the salivary glands of Podisus nigrispinus (Hemiptera: Pentatomidae). 2014. 47 f. Tese (Doutorado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2014.
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dc.publisher.department.fl_str_mv Ciência entomológica; Tecnologia entomológica
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