Ecofago 017 atua na prevenção e degradação de biofilme de Escherichia coli

• Autor(a) principal: Ribeiro, Karla Veloso Gonçalves
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2347
• topic: Biofilme; Bactéria; Bacteriófago; Biofilm; Bacteria; Bacteriophage; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

The bacteria are usually found attached to surfaces forming dense microbial communities called biofilms. The formation of biofilms has been documented as a survival strategy of microorganisms conferring resistance to antibiotics, disinfectants, biocides and the host immune system. The aim of this study at investigating the ability of the lytic bacteriophage Ecofago 017 to prevent the biofilm formation by Escherichia coli and degrade established biofilms. The conditions for growth and development of biofilms were evaluated and standardized. Different concentrations of phage were tested to define the optimal to prevention of biofilm formation and degradation. Analysis by Scanning Electron Microscopy and Confocal Microscopy was performed to support these results. We observed that strains of E. coli 30 biofilm have formed in air-liquid interface in the conditions tested, with higher production after 48 h. The experiments showed that virtually all concentrations of bacteriophage Ecophage 017 reduced both bacterial growths, as the amount of biofilm formed. The minimum dose able to significantly reduce the biofilm was 10 2 PFU / mL (Multiplicity of infection, MOI, 10-5), lower than described in literature. Experiments of degradation showed up to 85% reduction in biofilm mass after exposure to the phage, suggesting this is able to significantly reduce established E. coli 30 biofilms, indicating its ability to access and infect bacteria on the inside, causing cell death. Therefore the results show that the Ecophage 017 could be used both to reduce the fixing as to lyse bacterial cells associated with biofilms, generating biotechnological potential applicabilities to this phage.