Uso de condrócitos heterólogos cultivados na reparação de falhas osteocondrais produzidas no joelho de coelhos

• Main Author: Filgueiras, Richard da Rocha
• Publication Date: 2008
• Format: Doctoral thesis
• Language: por
• Source: LOCUS Repositório Institucional da UFV
• Download full: http://locus.ufv.br/handle/123456789/1428
• topic: Condrócitos; Catilagem; Cultura de tecidos; Chondrocytes; Cartilage; Tissue culture; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL

Chondrocytes removed from articular cartilage of nine healthy New Zealand White rabbits were cultivated and frozen, with cryoprotector DMSO 5% for six months. Phenotype and scanning electron microscopy analysis were performed in order to identify morphological and function differences between fresh and thawed cells. Thirty six New Zealand White rabbits, males and females, randomically divided in four groups of nine animals were used for the in vivo study. Circular osteochondral defects were induced on the right stifle, and were filled with fresh and thawed chondrocytes associated with platelet rich plasma (PRP). The present study demonstrates that, under the freezing conditions used, the use of heterologous chondrocytes is feasible. However, other studies using substrates that keep the chondrocytic phenotype and different concentrations of DMSO should be done to maintain a long term preserved chondrocyte bank. The association with PRP allows the cells to adhere to the site of injury and also provide growth factors that accelerate the repair with hyaline cartilage-like tissue. Clinically, the chondrocyte implantation associated with PRP reduces the articular pain and lameness due to capsular adherence inhibition and the integration of cartilaginous tissue on subchondral bone.