RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean

Detalhes bibliográficos
Autor(a) principal: Martins Filho, Sebastião
Data de Publicação: 2002
Outros Autores: Sediyama, Carlos Sigueyuki, Moreira, Maurilio Alves, Barros, Everaldo Gonçalves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1415-47572002000300012
http://locus.ufv.br//handle/123456789/26237
Resumo: The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross ‘Parana’ x ‘Bossier’, 2 RAPD markers were identified, CSOPA1800C and CSOPA21,250C, located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the resistance locus. DNA fragments of similar molecular weight were observed in the population derived from the cross ‘Cristalina’ x ‘Bossier’ at 2.3 ± 1.2 and 4.7 ± 1.5 cM from the resistance locus, respectively. In the offspring of the cross ‘Uberaba’ x ‘Bossier’, a DNA fragment corresponding to marker CSOPA1800C was detected at 5.6 ± 2.1 cM from the resistance locus. Although marker CSOPA21,250C was not observed in this population, an additional marker was detected (CSOUB11,100C) at 6.7 ± 2.2 cM from the resistance locus. The 1,250 bp fragment of CSOPA21,250C was cloned and converted into a SCAR marker, which amplified a single fragment whose size corresponded to the cloned segment of the crosses involving cultivars Cristalina and Parana. Markers CSOPA1800C, CSOPA21,250C and CSOUB11,100C were mapped to soybean linkage group J with the aid of known SSRs linked to the Rcs3 locus, indicating that the RAPD and SCAR markers identified in our research also tag this resistance gene.
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spelling Martins Filho, SebastiãoSediyama, Carlos SigueyukiMoreira, Maurilio AlvesBarros, Everaldo Gonçalves de2019-07-16T20:11:01Z2019-07-16T20:11:01Z20021678-4685http://dx.doi.org/10.1590/S1415-47572002000300012http://locus.ufv.br//handle/123456789/26237The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross ‘Parana’ x ‘Bossier’, 2 RAPD markers were identified, CSOPA1800C and CSOPA21,250C, located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the resistance locus. DNA fragments of similar molecular weight were observed in the population derived from the cross ‘Cristalina’ x ‘Bossier’ at 2.3 ± 1.2 and 4.7 ± 1.5 cM from the resistance locus, respectively. In the offspring of the cross ‘Uberaba’ x ‘Bossier’, a DNA fragment corresponding to marker CSOPA1800C was detected at 5.6 ± 2.1 cM from the resistance locus. Although marker CSOPA21,250C was not observed in this population, an additional marker was detected (CSOUB11,100C) at 6.7 ± 2.2 cM from the resistance locus. The 1,250 bp fragment of CSOPA21,250C was cloned and converted into a SCAR marker, which amplified a single fragment whose size corresponded to the cloned segment of the crosses involving cultivars Cristalina and Parana. Markers CSOPA1800C, CSOPA21,250C and CSOUB11,100C were mapped to soybean linkage group J with the aid of known SSRs linked to the Rcs3 locus, indicating that the RAPD and SCAR markers identified in our research also tag this resistance gene.engGenetics and Molecular Biologyv. 25, n. 3, p. 317- 321, 2002Molecular markersRAPDSCARSoybeanCercospora sojinaFrogeye leaf spotRAPD and SCAR markers linked to resistance to frogeye leaf spot in soybeaninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfartigoapplication/pdf331887https://locus.ufv.br//bitstream/123456789/26237/1/artigo.pdf077253b9e0fd499a78243f2728c2727cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/26237/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/262372019-07-16 17:18:14.437oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-07-16T20:18:14LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
title RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
spellingShingle RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
Martins Filho, Sebastião
Molecular markers
RAPD
SCAR
Soybean
Cercospora sojina
Frogeye leaf spot
title_short RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
title_full RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
title_fullStr RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
title_full_unstemmed RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
title_sort RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
author Martins Filho, Sebastião
author_facet Martins Filho, Sebastião
Sediyama, Carlos Sigueyuki
Moreira, Maurilio Alves
Barros, Everaldo Gonçalves de
author_role author
author2 Sediyama, Carlos Sigueyuki
Moreira, Maurilio Alves
Barros, Everaldo Gonçalves de
author2_role author
author
author
dc.contributor.author.fl_str_mv Martins Filho, Sebastião
Sediyama, Carlos Sigueyuki
Moreira, Maurilio Alves
Barros, Everaldo Gonçalves de
dc.subject.pt-BR.fl_str_mv Molecular markers
RAPD
SCAR
Soybean
Cercospora sojina
Frogeye leaf spot
topic Molecular markers
RAPD
SCAR
Soybean
Cercospora sojina
Frogeye leaf spot
description The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross ‘Parana’ x ‘Bossier’, 2 RAPD markers were identified, CSOPA1800C and CSOPA21,250C, located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the resistance locus. DNA fragments of similar molecular weight were observed in the population derived from the cross ‘Cristalina’ x ‘Bossier’ at 2.3 ± 1.2 and 4.7 ± 1.5 cM from the resistance locus, respectively. In the offspring of the cross ‘Uberaba’ x ‘Bossier’, a DNA fragment corresponding to marker CSOPA1800C was detected at 5.6 ± 2.1 cM from the resistance locus. Although marker CSOPA21,250C was not observed in this population, an additional marker was detected (CSOUB11,100C) at 6.7 ± 2.2 cM from the resistance locus. The 1,250 bp fragment of CSOPA21,250C was cloned and converted into a SCAR marker, which amplified a single fragment whose size corresponded to the cloned segment of the crosses involving cultivars Cristalina and Parana. Markers CSOPA1800C, CSOPA21,250C and CSOUB11,100C were mapped to soybean linkage group J with the aid of known SSRs linked to the Rcs3 locus, indicating that the RAPD and SCAR markers identified in our research also tag this resistance gene.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2019-07-16T20:11:01Z
dc.date.available.fl_str_mv 2019-07-16T20:11:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572002000300012
http://locus.ufv.br//handle/123456789/26237
dc.identifier.issn.none.fl_str_mv 1678-4685
identifier_str_mv 1678-4685
url http://dx.doi.org/10.1590/S1415-47572002000300012
http://locus.ufv.br//handle/123456789/26237
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 25, n. 3, p. 317- 321, 2002
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dc.publisher.none.fl_str_mv Genetics and Molecular Biology
publisher.none.fl_str_mv Genetics and Molecular Biology
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