Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Haroldo Silva
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4790
Resumo: Although widely known and rich in biodiversity, the Amazon still has a range of species that are underexplored and neglected by science, these species may have great potential for man. Only a fraction of the Amazon biodiversity is known. It is estimated that the plant wealth comprises about 30,000 species, about 10% of plants from all over the planet. The sacha inchi is a native plant from the Amazon, with great potential for the pharmaceutical and cosmetic industry. This plant has high amounts of monounsaturated and polyunsaturated fatty acids in the oil that is extracted from its seeds. Studies on the oil characteristics are found in scientific journals. On the other hand, there is a lack of studies that measure the genetic diversity among genotypes of sacha inchi. Embrapa Western Amazon, located in Manaus, has a sacha inchi Germplasm Bank which is composed of 37 accessions. This work was aimed to measure the genetic diversity within and between accessions in this collection through molecular markers and phenotypic traits related to fruits and seeds. AFLP markers were used and the results obtained the genetic dissimilarity was calculating by the arithmetic complement of Jaccard coefficient and subsequent construction cluster by using the UPGMA method. The phenotypic data collected was subjected to variance analysis, classified with average grouping by the Skott-Knott method, Mahalanobis distance method and clustered by the UPGMA method. Also performed canonical correlation analysis and the study of diversity within and between accessions using the method of AMOVA. The results showed the following; the existence of genetic variability among genotypes, the molecular analysis revealed geographic structure among accessions, the phenotypic data affirmed that there is great variation between means of most of the variables considered in this work, it was not possible to detect statistically significant correlations between variables related to fruit and variables related to seed tough.For breeding purposes, the exploration of diversity within the accessions and crosses between divergent accessions should be adopted.
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spelling Rodrigues, Haroldo Silvahttp://lattes.cnpq.br/5351164275640388Lopes, Maria Teresa Gomeshttp://lattes.cnpq.br/9307727443790246Oliveira, Aluízio Borém dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783555Z3Glasenapp, Jacqueline Siqueirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4737394P32015-03-26T13:42:30Z2014-01-232015-03-26T13:42:30Z2013-07-18RODRIGUES, Haroldo Silva. Genetic, morphological and agronomic characterization of sacha inchi germoplasm (Plukenetia volubilis L.) in the state of amazonas. 2013. 58 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2013.http://locus.ufv.br/handle/123456789/4790Although widely known and rich in biodiversity, the Amazon still has a range of species that are underexplored and neglected by science, these species may have great potential for man. Only a fraction of the Amazon biodiversity is known. It is estimated that the plant wealth comprises about 30,000 species, about 10% of plants from all over the planet. The sacha inchi is a native plant from the Amazon, with great potential for the pharmaceutical and cosmetic industry. This plant has high amounts of monounsaturated and polyunsaturated fatty acids in the oil that is extracted from its seeds. Studies on the oil characteristics are found in scientific journals. On the other hand, there is a lack of studies that measure the genetic diversity among genotypes of sacha inchi. Embrapa Western Amazon, located in Manaus, has a sacha inchi Germplasm Bank which is composed of 37 accessions. This work was aimed to measure the genetic diversity within and between accessions in this collection through molecular markers and phenotypic traits related to fruits and seeds. AFLP markers were used and the results obtained the genetic dissimilarity was calculating by the arithmetic complement of Jaccard coefficient and subsequent construction cluster by using the UPGMA method. The phenotypic data collected was subjected to variance analysis, classified with average grouping by the Skott-Knott method, Mahalanobis distance method and clustered by the UPGMA method. Also performed canonical correlation analysis and the study of diversity within and between accessions using the method of AMOVA. The results showed the following; the existence of genetic variability among genotypes, the molecular analysis revealed geographic structure among accessions, the phenotypic data affirmed that there is great variation between means of most of the variables considered in this work, it was not possible to detect statistically significant correlations between variables related to fruit and variables related to seed tough.For breeding purposes, the exploration of diversity within the accessions and crosses between divergent accessions should be adopted.Apesar de amplamente conhecida e rica em biodiversidade, a região amazônica ainda possui uma gama de espécies subexploradas e negligenciadas pela comunidade científica, espécies essas que apresentam grande potencial de utilização para o homem. Apenas uma pequena fração dessa biodiversidade é conhecida, estima-se que a riqueza da flora amazônica compreende aproximadamente 30.000 espécies, cerca de 10% das plantas de todo o planeta. A sacha inchi é uma espécie nativa da amazônica com grande potencial de uso para a indústria farmacêutica e estética, pois possui alto teor de ácidos graxos mono e poliinsaturados no óleo extraído de sua semente. Apesar de estudos sobre características desse óleo serem encontrados na literatura, existe uma grande carência de trabalhos que mensurem a diversidade genética entre genótipos de sacha inchi. A Embrapa Amazônia Ocidental, situada no município de Manaus, possui um Banco de Germoplasma de sacha inchi composto por 37 acessos. O presente trabalho teve como objetivo estudar a diversidade genética entre e dentro dos acessos componentes dessa coleção por meio de marcadores moleculares e características fenotípicas relacionadas à produção de frutos e sementes. Foram utilizados marcadores AFLP e os resultados obtidos, submetidos à análise de dissimilaridade por meio do cálculo do complemento aritmético do coeficiente de Jaccard e subsequente construção dendograma pelo método UPGMA. Os dados fenotípicos coletados foram submetidos à analise de variância e subsequente analise de agrupamento de médias pelo método Skott-Knott, distância generalizada de Mahalanobis e dendograma construído pelo método UPGMA. Foi realizada ainda a análise de correlações canônicas e estudo da diversidade entre e dentro dos acessos utilizando o método da AMOVA. Os resultados obtidos mostram a existência de variabilidade genética entre os acessos estudados, o estudo à nível molecular mostrou estruturação geográfica entre os acessos estudados, os dados fenotípicos mostram que existe grande amplitude entre as médias de maioria das variáveis consideradas neste trabalho, por outro lado não foi possível detectar correlações estatisticamente significativas entre variáveis relacionadas ao fruto e variáveis relacionadas à semente. Para fins de melhoramento genético deve-se adotar a exploração da diversidade dentro dos acessos e optar por cruzamentos entre acessos divergentes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Genética e MelhoramentoUFVBRGenética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; MePlukenetia volubilisPré-melhoramentoEuphorbiaceaeAmazôniaPlukenetia volubilis LPre-breedingEuphorbiaceaeAmazonCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETALCaracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do AmazonasGenetic, morphological and agronomic characterization of sacha inchi germoplasm (Plukenetia volubilis L.) in the state of amazonasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf759469https://locus.ufv.br//bitstream/123456789/4790/1/texto%20completo.pdf33ae9554924588966908ce701f540e81MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain92447https://locus.ufv.br//bitstream/123456789/4790/2/texto%20completo.pdf.txtb26e71b8c03d10eec3b4263b80aedcfcMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3586https://locus.ufv.br//bitstream/123456789/4790/3/texto%20completo.pdf.jpg9ee2b191de07b6ab00e93bf8e3861717MD53123456789/47902016-04-10 23:16:12.33oai:locus.ufv.br:123456789/4790Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:16:12LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas
dc.title.alternative.eng.fl_str_mv Genetic, morphological and agronomic characterization of sacha inchi germoplasm (Plukenetia volubilis L.) in the state of amazonas
title Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas
spellingShingle Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas
Rodrigues, Haroldo Silva
Plukenetia volubilis
Pré-melhoramento
Euphorbiaceae
Amazônia
Plukenetia volubilis L
Pre-breeding
Euphorbiaceae
Amazon
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
title_short Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas
title_full Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas
title_fullStr Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas
title_full_unstemmed Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas
title_sort Caracterização genética, morfológica e agronômica de germoplasma de sacha inchi (plukenetia volubilis L.) no estado do Amazonas
author Rodrigues, Haroldo Silva
author_facet Rodrigues, Haroldo Silva
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/5351164275640388
dc.contributor.author.fl_str_mv Rodrigues, Haroldo Silva
dc.contributor.advisor-co1.fl_str_mv Lopes, Maria Teresa Gomes
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/9307727443790246
dc.contributor.advisor1.fl_str_mv Oliveira, Aluízio Borém de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783555Z3
dc.contributor.referee1.fl_str_mv Glasenapp, Jacqueline Siqueira
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4737394P3
contributor_str_mv Lopes, Maria Teresa Gomes
Oliveira, Aluízio Borém de
Glasenapp, Jacqueline Siqueira
dc.subject.por.fl_str_mv Plukenetia volubilis
Pré-melhoramento
Euphorbiaceae
Amazônia
topic Plukenetia volubilis
Pré-melhoramento
Euphorbiaceae
Amazônia
Plukenetia volubilis L
Pre-breeding
Euphorbiaceae
Amazon
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
dc.subject.eng.fl_str_mv Plukenetia volubilis L
Pre-breeding
Euphorbiaceae
Amazon
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
description Although widely known and rich in biodiversity, the Amazon still has a range of species that are underexplored and neglected by science, these species may have great potential for man. Only a fraction of the Amazon biodiversity is known. It is estimated that the plant wealth comprises about 30,000 species, about 10% of plants from all over the planet. The sacha inchi is a native plant from the Amazon, with great potential for the pharmaceutical and cosmetic industry. This plant has high amounts of monounsaturated and polyunsaturated fatty acids in the oil that is extracted from its seeds. Studies on the oil characteristics are found in scientific journals. On the other hand, there is a lack of studies that measure the genetic diversity among genotypes of sacha inchi. Embrapa Western Amazon, located in Manaus, has a sacha inchi Germplasm Bank which is composed of 37 accessions. This work was aimed to measure the genetic diversity within and between accessions in this collection through molecular markers and phenotypic traits related to fruits and seeds. AFLP markers were used and the results obtained the genetic dissimilarity was calculating by the arithmetic complement of Jaccard coefficient and subsequent construction cluster by using the UPGMA method. The phenotypic data collected was subjected to variance analysis, classified with average grouping by the Skott-Knott method, Mahalanobis distance method and clustered by the UPGMA method. Also performed canonical correlation analysis and the study of diversity within and between accessions using the method of AMOVA. The results showed the following; the existence of genetic variability among genotypes, the molecular analysis revealed geographic structure among accessions, the phenotypic data affirmed that there is great variation between means of most of the variables considered in this work, it was not possible to detect statistically significant correlations between variables related to fruit and variables related to seed tough.For breeding purposes, the exploration of diversity within the accessions and crosses between divergent accessions should be adopted.
publishDate 2013
dc.date.issued.fl_str_mv 2013-07-18
dc.date.available.fl_str_mv 2014-01-23
2015-03-26T13:42:30Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:42:30Z
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dc.identifier.citation.fl_str_mv RODRIGUES, Haroldo Silva. Genetic, morphological and agronomic characterization of sacha inchi germoplasm (Plukenetia volubilis L.) in the state of amazonas. 2013. 58 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2013.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4790
identifier_str_mv RODRIGUES, Haroldo Silva. Genetic, morphological and agronomic characterization of sacha inchi germoplasm (Plukenetia volubilis L.) in the state of amazonas. 2013. 58 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2013.
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