FILOGENIA CITOGENÉTICA E MOLECULAR DE Brycon devillei, Brycon ferox, Brycon insignis, Brycon opalinus e Brycon vermelha (CHARACIDAE: BRYCONINAE) DO LESTE DO BRASIL

• Autor(a) principal: Travenzoli, Natália Martins
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2280
• topic: Bacias costeiras do leste do Brasil; Bacia rio do Doce; Bacia rio Mucuri; Bacia rio Paraíba do Sul; Padrão Heterocromático; Padrão NORs; Coastal drainages of eastern Brazil; Rio do doce basin; Rio Mucuri basin; Rio Paraíba do Sul basin; Heterochromatic pattern; NORs pattern; CNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA

The genus Brycon is the main representative of the subfamily Bryconinae and its species occur in basins to the West and East of the Andes. In Brazil, Bryconinae species are distributed in the main drainages; because they are affected by anthropogenic impacts, most of these species are threatened. This study is a hypothesis test of whether Bryconinae of the eastern Brazilian coast are a phylogeographic unit, using standard cytogenetic techniques (Giemsa, argyrophylic-nucleolar organizer region -Ag-NORsdetection, and C-banding) and mitochondrial molecular markers (cytochrome oxidase subunit I COI- and mitochondrial ribosomal 16S) on Brycon devillei, Brycon ferox, Brycon insignis, Brycon opalinus e Brycon vermelha. All these species were 2n=50, as all other Bryconinae. However, their karyotypic formulae were species-specific: B. devillei (26m+22sm+2st), B. ferox (28m+18sm+4st), B. insignis (22m+20sm+8st), B. opalinus (24m+20sm+6st) e B. vermelha (24m+20sm+6st). All species present in coastal basins of eastern Brazil showed NORs on the first pair of subtelocentric chromosomes and the first pair of chromosomes of the karyotype showed non-equilocal heterochromatin, indicating that all these species, with the monotypic genus Henochilus form a group reciprocally monophyletic in relation to the continental species. The 16S allowed to recover phylogenetic relationships among the oldest species Bryconinae, and COI gene was used to reconstruct the phylogeny of the species of eastern Brazil. The pattern obtained for both genes corroborate the hypothesis of monophyly of species Bryconinae of coastal basins of eastern Brazil. This condition probably was caused by the long history of isolation of the eastern coastal basins in relation to continental basins.