Resposta de linfonodos de bovinos mestiços inoculados a campo com a vacina recombinante rSBm7462 anti Rhipicephalus (Boophilus) microplus

• Autor(a) principal: Martinez Rodriguez, Lorena Catalina
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5181
• topic: Imunologia veterinária; Proteínas recombinantes; Bovino - Parasito; Carrapato - Controle; Vacinas; Rhipicephalus microplus; Veterinary immunology; Recombinant proteins; Cattle - Parasito; Tick - Control; Vaccines; Rhipicephalus microplus; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL::PATOLOGIA CLINICA ANIMAL

The cattle tick, Rhipicephalus (Boophilus) microplus is responsible for significant economic losses in tropical and subtropical countries around the world. Nowadays, the chemical control for this parasite is affected by the resistance development to several commercial acaricides, which has been reported in many countries. Furthermore, the concerns generated by chemical remains in animal products and the environment makes from this control a problem of high level importance for the public health. Thus, the immunological control becomes a promising alternative because the easy administration, low costs and its contributions to the food security. The objective of this study was to evaluate the lymph nodes response of cattle immunized at field with the peptide rSBm7462 anti Rhipicephalus (Boophilus) microplus. Fourteen crossbred cattle (Bos taurus x Bos indicus) with degree of blood holstein-zebu and aged 4-10 months were used. The animals were maintained on two farms in the north of Minas Gerais and received three immunizations with the peptide rSBm7462 applied subcutaneously at 30-day intervals. Pre-scapular lymph nodes were collected surgically 15 days after each immunization and fixed in formalin for 18 hours, then they were embedded in paraffin subsequently. Lymph node samples were stained with Hematoxylin-Eosin (HE) for cellular events observation. On the other hand, in order to antigens identifying in immunized animals lymph nodes, the immunohistochemistry (IHC) with peroxidase-anti peroxidase (PAP) method was performed. Lymph node response of cattle inoculated was evaluated by analysis of germinal centers (GC) formation, medullary cords hyperplasia (MC) and antigen rSBm7462 presence in PAP+ cells. This study shows that the recombinant peptide rSBm7462 induces a T-dependent adaptive immune response on lymphoid tissues characterized by the affinity and immunological memory in the secondary lymphoid tissues structure formation.