LINE-1 methylation is positively associated with healthier lifestyle but inversely related to body fat mass in healthy young individuals

• Autor(a) principal: Marques-Rocha, José Luiz
• Data de Publicação: 2016
• Outros Autores: Milagro, Fermin I., Mansego, Maria Luisa, Mourão, Denise Machado, Martínez, J. Alfredo, Bressan, Josefina
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://dx.doi.org/10.1080/15592294.2015.1135286; http://www.locus.ufv.br/handle/123456789/12760
• topic: Adiposity; Biomarker; Diet; Epigenetics; IL-6; Inflammation; TNF-a

With the goal of investigating if epigenetic biomarkers from white blood cells (WBC) are associated with dietary, anthropometric, metabolic, inflammatory and oxidative stress parameters in young and apparently healthy individuals. We evaluated 156 individuals (91 women, 65 men; age: 23.1§ 3.5 years; body mass index: 22.0§2.9 kg/m 2 ) for anthropometric, biochemical and clinical markers, including some components of the antioxidant defense system and inflammatory response. DNA methylation of LINE-1, TNF-a and IL-6 and the expression of some genes related to the inflammatory process were analyzed in WBC. Adiposity was lower among individuals with higher LINE-1 methylation. On the contrary, body fat-free mass was higher among those with higher LINE-1 methylation. Individuals with higher LINE-1 methylation had higher daily intakes of calories, iron and riboflavin. However, those individuals who presented lower percentages of LINE-1 methylation reported higher intakes of copper, niacin and thiamin. Interestingly, the group with higher LINE-1 methylation had a lower percentage of current smokers and more individuals practicing sports. On the other hand, TNF-a methylation percentage was negatively associated with waist girth, waist-to-hip ratio and waist-to-stature ratio. Plasma TNF-a levels were lower in those individuals with higher TNF-a methylation. This study suggests that higher levels of LINE-1 and TNF-a methylation are associated with better indicators of adiposity status in healthy young individuals. In addition, energy and micronutrient intake, as well as a healthy lifestyle, may have a role in the regulation of DNA methylation in WBC and the subsequent metabolic changes may affect epigenetic biomarkers.