Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi

Detalhes bibliográficos
Autor(a) principal: Lima, Hyanameyka Evangelista de
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/1035
Resumo: Bacterial blight of cowpea (Vigna unguiculata) caused by Xanthomonas axonopodis pv. vignicola (Xav) have been occurred in Brazil, however, there is no available epidemiological information about this pathosystem. The objective of this study was to know some epidemiological aspects related to bacterial blight of cowpea through studies on greenhouse and field. To evaluate the severity of cowpea bacterial blight, two diagrammatic scales were prepared, one for cultivars with oval leaflets and another one to lanceolate leaflets, using the cultivars BRS Bragança and BRS Mazagão, respectively. The use of diagrammatic scales resulted in higher accuracy and precision of the estimatives performed, and the variance of the absolute errors in the assessment of disease presented values within acceptable limits. Greenhouse and field experiments were installed to evaluate the disease severity in BRS Bragança plants according to the inoculums concentrations of Xav. The severity and defoliation increased with increments of inoculum concentration. Xav was detected in seeds from plants grown in field using CCM medium independent of inoculum concentration. The bacteria incubation period decreased with the elevated leaf wetness period. We calculated the corrected severity of disease based on a combined measurement of severity and defoliation. The severity, the area under the disease progress curve and disease progress rate increased as the leaf wetness period elevated. Soybean (Glycine max) cv. BRS Tracajá, common bean (Phaseolus vulgaris) cv. Pérola and weed species (Euphorbia heterophylla) showed no disease symptoms when inoculated with Xav (107 CFU.mL-1) by spraying. However, common bean plants showed hypersensitivity reactions (HR). All plants presented HR when Xav was infiltrated with a syringe in their tissues, and total tissue necrosis was observed in plants of E. heterophylla at less than 24 hours after inoculation. Other test was also conducted in greenhouse to assess the disease severity at different stages of development of cowpea plants (primary leaves - PL, 2 or 3 trifoliate leaves TL, with open flowers OF, and with pod development - PD) on the time of infection, as well as its influence on defoliation, on components of production and on transmission of bacteria to the seeds. The disease caused defoliation in all cowpea plants, regardless of phenologic stage of the culture and there were significant differences in relative area under the disease progress curve (RAUDPC) among development stages, helping to reduce production of some components, especially of the mass of dry grains (MDG). It was possible to detect Xav in seeds of cowpea plants infected at any development stage and was detected higher amount of CFU.g-1 seed in seeds samples from plants inoculated by Xav on PD stage and TL stage. Subsequently, two field experiments were conducted in two consecutive seasons, for assessing the disease severity, the components of cowpea production and the transmissibility of the bacteria to the seeds. The cultivars used were BRS Bragança and BRS Vita-7, which were inoculated by Xav when de plants were in TL stage and OF stage. The disease severity was higher in BRS Bragança than BRS Vita-7, and there were reductions in some components of production, mainly in MDG. There were differences (p ≤ 0,05) by t test in RAUDPC between TL stage and OF stage in plants BRS Bragança inoculated by Xav. It was possible to detect Xav in seeds of cowpea of two cowpea cultivars, regardless of development stage in which they were inoculated, being detected higher amount of CFU g-1 seed in seeds samples from plants of cultivar BRS Bragança on TL stage inoculated by Xav.
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spelling Lima, Hyanameyka Evangelista dehttp://lattes.cnpq.br/8187034668235348Nechet, Kátia de Limahttp://lattes.cnpq.br/7435457545190771Vieira, Bernardo de Almeida Halfeldhttp://lattes.cnpq.br/3885880335229932Oliveira, José Rogério dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785966E6Paula Júnior, Trazilbo José dehttp://lattes.cnpq.br/7899276097018876Carneiro, José Eustáquio de Souzahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783648T92015-03-26T12:41:56Z2012-04-192015-03-26T12:41:56Z2011-07-22LIMA, Hyanameyka Evangelista de. Epidemiology of cowpea bacterial blight (Xanthomonas axonopodis pv. vignicola). 2011. 108 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011.http://locus.ufv.br/handle/123456789/1035Bacterial blight of cowpea (Vigna unguiculata) caused by Xanthomonas axonopodis pv. vignicola (Xav) have been occurred in Brazil, however, there is no available epidemiological information about this pathosystem. The objective of this study was to know some epidemiological aspects related to bacterial blight of cowpea through studies on greenhouse and field. To evaluate the severity of cowpea bacterial blight, two diagrammatic scales were prepared, one for cultivars with oval leaflets and another one to lanceolate leaflets, using the cultivars BRS Bragança and BRS Mazagão, respectively. The use of diagrammatic scales resulted in higher accuracy and precision of the estimatives performed, and the variance of the absolute errors in the assessment of disease presented values within acceptable limits. Greenhouse and field experiments were installed to evaluate the disease severity in BRS Bragança plants according to the inoculums concentrations of Xav. The severity and defoliation increased with increments of inoculum concentration. Xav was detected in seeds from plants grown in field using CCM medium independent of inoculum concentration. The bacteria incubation period decreased with the elevated leaf wetness period. We calculated the corrected severity of disease based on a combined measurement of severity and defoliation. The severity, the area under the disease progress curve and disease progress rate increased as the leaf wetness period elevated. Soybean (Glycine max) cv. BRS Tracajá, common bean (Phaseolus vulgaris) cv. Pérola and weed species (Euphorbia heterophylla) showed no disease symptoms when inoculated with Xav (107 CFU.mL-1) by spraying. However, common bean plants showed hypersensitivity reactions (HR). All plants presented HR when Xav was infiltrated with a syringe in their tissues, and total tissue necrosis was observed in plants of E. heterophylla at less than 24 hours after inoculation. Other test was also conducted in greenhouse to assess the disease severity at different stages of development of cowpea plants (primary leaves - PL, 2 or 3 trifoliate leaves TL, with open flowers OF, and with pod development - PD) on the time of infection, as well as its influence on defoliation, on components of production and on transmission of bacteria to the seeds. The disease caused defoliation in all cowpea plants, regardless of phenologic stage of the culture and there were significant differences in relative area under the disease progress curve (RAUDPC) among development stages, helping to reduce production of some components, especially of the mass of dry grains (MDG). It was possible to detect Xav in seeds of cowpea plants infected at any development stage and was detected higher amount of CFU.g-1 seed in seeds samples from plants inoculated by Xav on PD stage and TL stage. Subsequently, two field experiments were conducted in two consecutive seasons, for assessing the disease severity, the components of cowpea production and the transmissibility of the bacteria to the seeds. The cultivars used were BRS Bragança and BRS Vita-7, which were inoculated by Xav when de plants were in TL stage and OF stage. The disease severity was higher in BRS Bragança than BRS Vita-7, and there were reductions in some components of production, mainly in MDG. There were differences (p ≤ 0,05) by t test in RAUDPC between TL stage and OF stage in plants BRS Bragança inoculated by Xav. It was possible to detect Xav in seeds of cowpea of two cowpea cultivars, regardless of development stage in which they were inoculated, being detected higher amount of CFU g-1 seed in seeds samples from plants of cultivar BRS Bragança on TL stage inoculated by Xav.A mancha-bacteriana do feijão-caupi (Vigna unguiculata), causada por Xanthomonas axonopodis pv. vignicola (Xav), tem ocorrido no Brasil, mas não se dispõe de informações epidemiológicas sobre este patossistema. O objetivo deste trabalho foi conhecer alguns aspectos epidemiológicos relacionados a esta doença por meio de estudos em casa-de-vegetação e campo. Para avaliar a severidade da mancha bacteriana do feijão-caupi foram elaboradas duas escalas diagramáticas, uma para cultivares com folíolos ovais e outra para folíolos lanceolados, utilizando-se as cultivares BRS Bragança e BRS Mazagão, respectivamente. As escalas diagramáticas propostas permitiram quantificar os sintomas da doença de forma acurada, precisa e reproduzível. Em casa-de-vegetação e campo foram instalados experimentos visando avaliar a severidade da doença em plantas da cv. BRS Bragança em função da concentração de inóculo de Xav. Houve aumento da severidade e desfolha com o incremento da concentração de inóculo. Xav foi detectada em sementes provenientes de plantas cultivadas em campo no meio semi-seletivo CCM independente da concentração de inóculo. O período de incubação da bactéria diminuiu com o aumento do período de molhamento foliar (PMF). Calculou-se a severidade corrigida com base na desfolha causada pela doença e observou-se aumento da severidade com o aumento do PMF. A mesma tendência foi obtida para a área abaixo da curva de progresso da doença e a taxa de progresso da doença. Plantas de soja (Glycine max) cv. BRS Tracajá, feijão-comum (Phaseolus vulgaris) cv. Pérola e leiteira (Euphorbia heterophylla) não apresentaram sintomas da doença quando inoculadas com Xav (107 UFC.mL-1) por aspersão. Porém, plantas de feijão-comum apresentaram reação de hipersensibilidade (HR). Todas as plantas apresentaram HR quando Xav foi infiltrada em seus tecidos com seringa, sendo observado escurecimento total do tecido em menos de 24 horas após a inoculação em plantas de E. heterophylla. Realizou-se também ensaio em casa-de-vegetação para avaliar a severidade da doença em função de quatro estádios fenológicos do feijão-caupi (plantas com folhas primárias - EFP; com 2 a 3 trifólios - ET; em florescimento - EF e em formação de vagens - EV) na época de infecção, bem como sua influência na desfolha, nos componentes de produção e na transmissibilidade da bactéria para as sementes. A doença causou desfolha em todas as plantas de feijão-caupi, independente do estádio fenológico da cultura e houve diferença significativa na área abaixo da curva de progresso relativa da doença (AACPRD) entre os estádios fenológicos, contribuindo para a redução de alguns componentes de produção, principalmente o de massa de grãos secos (MGS). Foi possível isolar Xav de sementes de feijão-caupi provenientes de plantas infectadas em qualquer estádio fenológico, sendo detectada maior quantidade de UFC.g-1 de sementes em lotes provenientes de plantas inoculadas com Xav no EV e ET. Posteriormente, dois experimentos de campo foram conduzidos em estações seguidas de cultivo, utilizando-se as cultivares BRS Bragança e BRS Vita-7, que foram inoculadas com Xav no ET e no EF, a fim de avaliar os componentes de produção do feijão-caupi e a transmissibilidade da bactéria para as sementes. A severidade da doença foi maior em BRS Bragança do que em BRS Vita-7 e houve reduções de alguns componentes de produção, principalmente na MGS. Houve diferença (p≤0,05) pelo teste t na AACPRD entre o ET e o EF em plantas BRS Bragança inoculadas com Xav. Foi possível isolar Xav em sementes provenientes das duas cultivares de feijão-caupi, independente do estádio fenológico em que foram inoculadas, sendo detectada maior quantidade de UFC.g-1 em sementes provenientes de plantas da cv. BRS Bragança inoculadas com Xav no ET.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleXanthomonas axonopodis pv. vignicolaFeijãoEpidemiologiaRelação hospedeiro-parasitoXanthomonas axonopodis pv. vignicolaBeanEpidemiologyHost-parasite relationshipCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAEpidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupiEpidemiology of cowpea bacterial blight (Xanthomonas axonopodis pv. vignicola)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf2708157https://locus.ufv.br//bitstream/123456789/1035/1/texto%20completo.pdf04c6025c09213c99fa7f309bd6942077MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain208451https://locus.ufv.br//bitstream/123456789/1035/2/texto%20completo.pdf.txt1eb8920e33c5414f7fb481277cde53c0MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3603https://locus.ufv.br//bitstream/123456789/1035/3/texto%20completo.pdf.jpg5273dd7e7d3d234095ceb879b18a0985MD53123456789/10352016-04-06 23:15:49.126oai:locus.ufv.br:123456789/1035Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:15:49LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi
dc.title.alternative.eng.fl_str_mv Epidemiology of cowpea bacterial blight (Xanthomonas axonopodis pv. vignicola)
title Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi
spellingShingle Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi
Lima, Hyanameyka Evangelista de
Xanthomonas axonopodis pv. vignicola
Feijão
Epidemiologia
Relação hospedeiro-parasito
Xanthomonas axonopodis pv. vignicola
Bean
Epidemiology
Host-parasite relationship
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi
title_full Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi
title_fullStr Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi
title_full_unstemmed Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi
title_sort Epidemiologia da mancha-bacteriana (Xanthomonas axonopodis pv. vignicola) do feijão-caupi
author Lima, Hyanameyka Evangelista de
author_facet Lima, Hyanameyka Evangelista de
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/8187034668235348
dc.contributor.author.fl_str_mv Lima, Hyanameyka Evangelista de
dc.contributor.advisor-co1.fl_str_mv Nechet, Kátia de Lima
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/7435457545190771
dc.contributor.advisor-co2.fl_str_mv Vieira, Bernardo de Almeida Halfeld
dc.contributor.advisor-co2Lattes.fl_str_mv http://lattes.cnpq.br/3885880335229932
dc.contributor.advisor1.fl_str_mv Oliveira, José Rogério de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785966E6
dc.contributor.referee1.fl_str_mv Paula Júnior, Trazilbo José de
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/7899276097018876
dc.contributor.referee2.fl_str_mv Carneiro, José Eustáquio de Souza
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783648T9
contributor_str_mv Nechet, Kátia de Lima
Vieira, Bernardo de Almeida Halfeld
Oliveira, José Rogério de
Paula Júnior, Trazilbo José de
Carneiro, José Eustáquio de Souza
dc.subject.por.fl_str_mv Xanthomonas axonopodis pv. vignicola
Feijão
Epidemiologia
Relação hospedeiro-parasito
topic Xanthomonas axonopodis pv. vignicola
Feijão
Epidemiologia
Relação hospedeiro-parasito
Xanthomonas axonopodis pv. vignicola
Bean
Epidemiology
Host-parasite relationship
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Xanthomonas axonopodis pv. vignicola
Bean
Epidemiology
Host-parasite relationship
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description Bacterial blight of cowpea (Vigna unguiculata) caused by Xanthomonas axonopodis pv. vignicola (Xav) have been occurred in Brazil, however, there is no available epidemiological information about this pathosystem. The objective of this study was to know some epidemiological aspects related to bacterial blight of cowpea through studies on greenhouse and field. To evaluate the severity of cowpea bacterial blight, two diagrammatic scales were prepared, one for cultivars with oval leaflets and another one to lanceolate leaflets, using the cultivars BRS Bragança and BRS Mazagão, respectively. The use of diagrammatic scales resulted in higher accuracy and precision of the estimatives performed, and the variance of the absolute errors in the assessment of disease presented values within acceptable limits. Greenhouse and field experiments were installed to evaluate the disease severity in BRS Bragança plants according to the inoculums concentrations of Xav. The severity and defoliation increased with increments of inoculum concentration. Xav was detected in seeds from plants grown in field using CCM medium independent of inoculum concentration. The bacteria incubation period decreased with the elevated leaf wetness period. We calculated the corrected severity of disease based on a combined measurement of severity and defoliation. The severity, the area under the disease progress curve and disease progress rate increased as the leaf wetness period elevated. Soybean (Glycine max) cv. BRS Tracajá, common bean (Phaseolus vulgaris) cv. Pérola and weed species (Euphorbia heterophylla) showed no disease symptoms when inoculated with Xav (107 CFU.mL-1) by spraying. However, common bean plants showed hypersensitivity reactions (HR). All plants presented HR when Xav was infiltrated with a syringe in their tissues, and total tissue necrosis was observed in plants of E. heterophylla at less than 24 hours after inoculation. Other test was also conducted in greenhouse to assess the disease severity at different stages of development of cowpea plants (primary leaves - PL, 2 or 3 trifoliate leaves TL, with open flowers OF, and with pod development - PD) on the time of infection, as well as its influence on defoliation, on components of production and on transmission of bacteria to the seeds. The disease caused defoliation in all cowpea plants, regardless of phenologic stage of the culture and there were significant differences in relative area under the disease progress curve (RAUDPC) among development stages, helping to reduce production of some components, especially of the mass of dry grains (MDG). It was possible to detect Xav in seeds of cowpea plants infected at any development stage and was detected higher amount of CFU.g-1 seed in seeds samples from plants inoculated by Xav on PD stage and TL stage. Subsequently, two field experiments were conducted in two consecutive seasons, for assessing the disease severity, the components of cowpea production and the transmissibility of the bacteria to the seeds. The cultivars used were BRS Bragança and BRS Vita-7, which were inoculated by Xav when de plants were in TL stage and OF stage. The disease severity was higher in BRS Bragança than BRS Vita-7, and there were reductions in some components of production, mainly in MDG. There were differences (p ≤ 0,05) by t test in RAUDPC between TL stage and OF stage in plants BRS Bragança inoculated by Xav. It was possible to detect Xav in seeds of cowpea of two cowpea cultivars, regardless of development stage in which they were inoculated, being detected higher amount of CFU g-1 seed in seeds samples from plants of cultivar BRS Bragança on TL stage inoculated by Xav.
publishDate 2011
dc.date.issued.fl_str_mv 2011-07-22
dc.date.available.fl_str_mv 2012-04-19
2015-03-26T12:41:56Z
dc.date.accessioned.fl_str_mv 2015-03-26T12:41:56Z
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dc.identifier.citation.fl_str_mv LIMA, Hyanameyka Evangelista de. Epidemiology of cowpea bacterial blight (Xanthomonas axonopodis pv. vignicola). 2011. 108 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/1035
identifier_str_mv LIMA, Hyanameyka Evangelista de. Epidemiology of cowpea bacterial blight (Xanthomonas axonopodis pv. vignicola). 2011. 108 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011.
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