Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa

Detalhes bibliográficos
Autor(a) principal: Mendes, Giselle Camargo
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4314
Resumo: Plants acquired strategies I and II for iron absorption. Strategy I occurs in terrestrial plants and includes the reduction of iron in the ferric form (Fe+3) to ferrous form (Fe+2). Strategy II of iron absorption is present in grasses and is characterized by the release of phytosiderophores to the environment that chelate ferric iron. The grain filling process in cereals involves the transfer of assimilates directly from the xylem to the grain and also remobilization of assimilates from vegetative organs through the phloem. The aim of this work was to study the pattern of accumulated nutrients in seeds of rice genotypes cultivated in two regions of Brazil and to study the pattern and correlations among the nutrients accumulated in the grain. Moreover, it was verified the occurrence of transcripts homologous to rice (Oryza sativa) gene families known to be related to iron homeostasis in ESTs databases of Sorghum bicolor, Zea mays, Triticum aestivum and Saccharum officinalis and in a database containing only maize (Z. mays) endosperm ESTs. These analyses pointed to gene families that are functionally expressed in these species and specifically during the formation of the maize grain. For the analysis of accumulation of nutrients in the grain it was used ICP-MS and the data were subjected to statistical analysis: ANOVA, Pearson correlation and path analysis. The Pearson correlation results of accumulated nutrients in the grain do not demonstrated the direct and indirect effects of nutrients accumulated. On the other hand, the path analysis of these same data revealed a direct correlation of Fe with P, S and Ca in the experiment conducted in MG and direct correlation of Fe with P in the experiment conducted in the RS. The data of correlation between iron content in flag leaves and panicle of rice in different stages of development showed positive correlation. Moreover, the levels of Fe accumulated in the grain of the genotypes grown in MG were higher compared to those grown in RS. To verify the occurrence of homology, the amino acid sequences related to the rice transcripts were obtained from NCBI and KOME databases. The sequences of ESTs from other grass species were obtained from TIGR-Plant transcripts assemblies program and the maize endosperm database was obtained from NCBI. The genes were aligned using the program BLAST2 with TBLASTN tool with e-value of 1e-10 and minimal identity of 40%. It was found counterparts of the gene families studied in all species evaluated. The NAS gene family, related to phytosiderophores synthesis showed high conservation (about 70%) among Poaceae family members studied, except wheat and maize endosperm. The OsFER1 gene showed high conservation (80%) among all members of Poaceae. This suggests the importance of these gene families in the transport and accumulation of iron among the Poaceae species. It was found representatives from each one of the families IRT1, YSL and ZIP in maize endosperm which suggests that these genes may be functionally expressed during the formation of maize grain.
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spelling Mendes, Giselle Camargohttp://lattes.cnpq.br/4507854638255402Oliveira, Antonio Costa dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780819H4Cano, Marco Antonio Olivahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787546T4Almeida, Andréa Miyasaka dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792501H4Loureiro, Marcelo Ehlershttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780851Y3Cambraia, Joséhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783868U6Cruz, Cosme Damiãohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788274A62015-03-26T13:36:40Z2015-03-062015-03-26T13:36:40Z2009-03-20MENDES, Giselle Camargo. Environmental and genotypic effects on nutrient content of rice seeds, Oryza sativa. 2009. 103 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2009.http://locus.ufv.br/handle/123456789/4314Plants acquired strategies I and II for iron absorption. Strategy I occurs in terrestrial plants and includes the reduction of iron in the ferric form (Fe+3) to ferrous form (Fe+2). Strategy II of iron absorption is present in grasses and is characterized by the release of phytosiderophores to the environment that chelate ferric iron. The grain filling process in cereals involves the transfer of assimilates directly from the xylem to the grain and also remobilization of assimilates from vegetative organs through the phloem. The aim of this work was to study the pattern of accumulated nutrients in seeds of rice genotypes cultivated in two regions of Brazil and to study the pattern and correlations among the nutrients accumulated in the grain. Moreover, it was verified the occurrence of transcripts homologous to rice (Oryza sativa) gene families known to be related to iron homeostasis in ESTs databases of Sorghum bicolor, Zea mays, Triticum aestivum and Saccharum officinalis and in a database containing only maize (Z. mays) endosperm ESTs. These analyses pointed to gene families that are functionally expressed in these species and specifically during the formation of the maize grain. For the analysis of accumulation of nutrients in the grain it was used ICP-MS and the data were subjected to statistical analysis: ANOVA, Pearson correlation and path analysis. The Pearson correlation results of accumulated nutrients in the grain do not demonstrated the direct and indirect effects of nutrients accumulated. On the other hand, the path analysis of these same data revealed a direct correlation of Fe with P, S and Ca in the experiment conducted in MG and direct correlation of Fe with P in the experiment conducted in the RS. The data of correlation between iron content in flag leaves and panicle of rice in different stages of development showed positive correlation. Moreover, the levels of Fe accumulated in the grain of the genotypes grown in MG were higher compared to those grown in RS. To verify the occurrence of homology, the amino acid sequences related to the rice transcripts were obtained from NCBI and KOME databases. The sequences of ESTs from other grass species were obtained from TIGR-Plant transcripts assemblies program and the maize endosperm database was obtained from NCBI. The genes were aligned using the program BLAST2 with TBLASTN tool with e-value of 1e-10 and minimal identity of 40%. It was found counterparts of the gene families studied in all species evaluated. The NAS gene family, related to phytosiderophores synthesis showed high conservation (about 70%) among Poaceae family members studied, except wheat and maize endosperm. The OsFER1 gene showed high conservation (80%) among all members of Poaceae. This suggests the importance of these gene families in the transport and accumulation of iron among the Poaceae species. It was found representatives from each one of the families IRT1, YSL and ZIP in maize endosperm which suggests that these genes may be functionally expressed during the formation of maize grain.As plantas adquiriram estratégias I e II de absorção do ferro. A estratégia I ocorre em plantas terrestres e inclui a redução do ferro da forma férrica (Fe+3) para a forma ferrosa (Fe+2). A estratégia II de absorção de ferro está presente nas gramíneas e é caracterizada pela liberação de fitosiderósforos no ambiente que se complexam ao íon férrico. O processo de enchimento do grão em cereais envolve a transferência de assimilados diretamente do xilema para o grão e também a remobilização de assimilados de órgãos vegetativos, através do floema. O objetivo do presente trabalho foi estudar o padrão de nutrientes acumulados nas sementes de genótipos de arroz cultivados em duas regiões do Brasil e estudar o padrão e as correlações entre os nutrientes acumulados no grão. Além disso, foi verificada a ocorrência de transcritos homólogos a famílias gênicas de arroz (Oryza sativa) conhecidamente relacionadas com homeostase de ferro em bancos de ESTs de Sorghum bicolor, Zea mays, Triticum aestivum e Saccharum officinalis e em um banco contendo apenas ESTs de endosperma de milho (Z. mays). Estas análises permitiram prever quais famílias gênicas são funcionalmente expressas nestas espécies e especificamente durante a formação do grão de milho. Para as analises de acúmulo de nutrientes no grão foi utilizado o ICP-MS e os dados foram submetidos às analises estatísticas: ANOVA, correlação de Pearson e análise de trilha. Os resultados de correlação de Pearson dos nutrientes acumulados no grão não evidenciaram os efeitos diretos e indiretos dos nutrientes acumulados. Por outro lado a análise de trilha destes mesmos resultados demonstrou efeitos de correlação direta do Fe com P, S e Ca no experimento realizado em MG e correlação direta do Fe com P no experimento realizado no RS. Os dados de correlação entre teor de ferro acumulado nas folhas bandeiras de arroz e nas panículas nos diferentes estádios de desenvolvimento tiveram correlação positiva. Além disso, os teores de Fe acumulado no grão dos genótipos cultivados em MG foram superiores comparados aos cultivados no RS. Para verificar a ocorrência de homologia, as seqüências de aminoácidos referentes aos transcritos em arroz foram obtidas a partir do banco de dados NCBI e KOME. As seqüências de ESTs de banco de dados das espécies de gramíneas foram obtidas a partir do programa TIGRPlant transcripts assemblies e do banco de endosperma de milho foi obtido do NCBI. Os genes foram alinhados utilizando o programa BLAST2 com a ferramenta TBLASTN com e-value de 1e-10 e identidade mínima de 40%. Foram encontrados homólogos das famílias gênicas estudadas em todas as espécies avaliadas. A família gênica NAS, relacionada com a síntese de fitosideróforos, possui alta conservação (cerca 70%) entre os genes da família Poaceae estudada, com exceção do trigo e endosperma de milho. O gene OsFER1 apresentou alta conservação (cerca de 80%) entre todos os genes de Poaceae. Isto indica a importância dessas famílias gênicas no transporte e acúmulo de ferro entre as espécies de Poaceae. Foram encontradas representantes de cada uma das famílias IRT1, YSL e ZIP no endosperma de milho o que sugere que esses genes possam ser funcionalmente expressos durante a formação de grãos em milho.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em Fisiologia VegetalUFVBRControle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superioresArrozFerroBiofortificaçãoRiceIronBiofortificationCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADASEfeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativaEnvironmental and genotypic effects on nutrient content of rice seeds, Oryza sativainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/embargoedAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf890349https://locus.ufv.br//bitstream/123456789/4314/1/texto%20completo.pdf3a3c4b6898edfb8ec6ee997137629c1eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain226537https://locus.ufv.br//bitstream/123456789/4314/2/texto%20completo.pdf.txtc77ee7604a025e943d6ad3b322657162MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3556https://locus.ufv.br//bitstream/123456789/4314/3/texto%20completo.pdf.jpge845b2c0c24b87cbd803b4867025bd33MD53123456789/43142016-04-10 23:07:10.54oai:locus.ufv.br:123456789/4314Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:07:10LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa
dc.title.alternative.eng.fl_str_mv Environmental and genotypic effects on nutrient content of rice seeds, Oryza sativa
title Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa
spellingShingle Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa
Mendes, Giselle Camargo
Arroz
Ferro
Biofortificação
Rice
Iron
Biofortification
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADAS
title_short Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa
title_full Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa
title_fullStr Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa
title_full_unstemmed Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa
title_sort Efeito ambiental e genotípico no teor de nutrientes acumulados em sementes de arroz, Oryza sativa
author Mendes, Giselle Camargo
author_facet Mendes, Giselle Camargo
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/4507854638255402
dc.contributor.author.fl_str_mv Mendes, Giselle Camargo
dc.contributor.advisor-co1.fl_str_mv Oliveira, Antonio Costa de
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780819H4
dc.contributor.advisor-co2.fl_str_mv Cano, Marco Antonio Oliva
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787546T4
dc.contributor.advisor1.fl_str_mv Almeida, Andréa Miyasaka de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792501H4
dc.contributor.referee1.fl_str_mv Loureiro, Marcelo Ehlers
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780851Y3
dc.contributor.referee2.fl_str_mv Cambraia, José
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783868U6
dc.contributor.referee3.fl_str_mv Cruz, Cosme Damião
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788274A6
contributor_str_mv Oliveira, Antonio Costa de
Cano, Marco Antonio Oliva
Almeida, Andréa Miyasaka de
Loureiro, Marcelo Ehlers
Cambraia, José
Cruz, Cosme Damião
dc.subject.por.fl_str_mv Arroz
Ferro
Biofortificação
topic Arroz
Ferro
Biofortificação
Rice
Iron
Biofortification
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADAS
dc.subject.eng.fl_str_mv Rice
Iron
Biofortification
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::FISIOLOGIA DE PLANTAS CULTIVADAS
description Plants acquired strategies I and II for iron absorption. Strategy I occurs in terrestrial plants and includes the reduction of iron in the ferric form (Fe+3) to ferrous form (Fe+2). Strategy II of iron absorption is present in grasses and is characterized by the release of phytosiderophores to the environment that chelate ferric iron. The grain filling process in cereals involves the transfer of assimilates directly from the xylem to the grain and also remobilization of assimilates from vegetative organs through the phloem. The aim of this work was to study the pattern of accumulated nutrients in seeds of rice genotypes cultivated in two regions of Brazil and to study the pattern and correlations among the nutrients accumulated in the grain. Moreover, it was verified the occurrence of transcripts homologous to rice (Oryza sativa) gene families known to be related to iron homeostasis in ESTs databases of Sorghum bicolor, Zea mays, Triticum aestivum and Saccharum officinalis and in a database containing only maize (Z. mays) endosperm ESTs. These analyses pointed to gene families that are functionally expressed in these species and specifically during the formation of the maize grain. For the analysis of accumulation of nutrients in the grain it was used ICP-MS and the data were subjected to statistical analysis: ANOVA, Pearson correlation and path analysis. The Pearson correlation results of accumulated nutrients in the grain do not demonstrated the direct and indirect effects of nutrients accumulated. On the other hand, the path analysis of these same data revealed a direct correlation of Fe with P, S and Ca in the experiment conducted in MG and direct correlation of Fe with P in the experiment conducted in the RS. The data of correlation between iron content in flag leaves and panicle of rice in different stages of development showed positive correlation. Moreover, the levels of Fe accumulated in the grain of the genotypes grown in MG were higher compared to those grown in RS. To verify the occurrence of homology, the amino acid sequences related to the rice transcripts were obtained from NCBI and KOME databases. The sequences of ESTs from other grass species were obtained from TIGR-Plant transcripts assemblies program and the maize endosperm database was obtained from NCBI. The genes were aligned using the program BLAST2 with TBLASTN tool with e-value of 1e-10 and minimal identity of 40%. It was found counterparts of the gene families studied in all species evaluated. The NAS gene family, related to phytosiderophores synthesis showed high conservation (about 70%) among Poaceae family members studied, except wheat and maize endosperm. The OsFER1 gene showed high conservation (80%) among all members of Poaceae. This suggests the importance of these gene families in the transport and accumulation of iron among the Poaceae species. It was found representatives from each one of the families IRT1, YSL and ZIP in maize endosperm which suggests that these genes may be functionally expressed during the formation of maize grain.
publishDate 2009
dc.date.issued.fl_str_mv 2009-03-20
dc.date.accessioned.fl_str_mv 2015-03-26T13:36:40Z
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dc.identifier.citation.fl_str_mv MENDES, Giselle Camargo. Environmental and genotypic effects on nutrient content of rice seeds, Oryza sativa. 2009. 103 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2009.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4314
identifier_str_mv MENDES, Giselle Camargo. Environmental and genotypic effects on nutrient content of rice seeds, Oryza sativa. 2009. 103 f. Dissertação (Mestrado em Controle da maturação e senescência em órgãos perecíveis; Fisiologia molecular de plantas superiores) - Universidade Federal de Viçosa, Viçosa, 2009.
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