Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/S0100-69912012000600013 http://www.locus.ufv.br/handle/123456789/16857 |
Resumo: | To analyze, through molecular biology, the diversity of the intestinal microbiota before and after resection of the ileocecal junction and reconstruction of intestinal transit with and without the creation of a neosphincter. Fourteen Wistar rats were divided into two groups: Group A (n = 7), submitted to resection of the ileocecal valve and end-to-end, single-layer ileocolic anastomosis; and Group B (n = 7) with resection of the ileocecal valve and end-to-end, single-layer ileocolic anastomosis followed by construction of an artificial sphincter. Intraluminal contents were collected from both groups. The animals were reoperated 20 days after the first procedure, with new collection of intraluminal contents of the ileum and colon. From the samples collected, DNA was extracted for PCR-DGGE. The electrophoretic banding patterns generated in the reaction were analyzed for similarities and diversities of the microbiota. The diversity of microorganisms was larger and in more samples when collected from the ileum than from the colon. The group with the neosphincter showed the highest variation in the colon, from 2.11 to 2.93. In three animals from each group w established similarity comparison, and they displayed no similarity with controls. ileocecal resection led to changes in ileal microbiota and, with the creation of new sphincter, the changes were even greater. |
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Barra, Ângela AparecidaFerreira, Alessandra BarbosaNagen, MarceloFreitas, Fernanda de SouzaFurtado, Maria Cristina VasconcelosSilva, Alcino Lázaro daRena, Cícero de Lima2018-01-26T12:11:42Z2018-01-26T12:11:42Z2012-06-201809-4546http://dx.doi.org/10.1590/S0100-69912012000600013http://www.locus.ufv.br/handle/123456789/16857To analyze, through molecular biology, the diversity of the intestinal microbiota before and after resection of the ileocecal junction and reconstruction of intestinal transit with and without the creation of a neosphincter. Fourteen Wistar rats were divided into two groups: Group A (n = 7), submitted to resection of the ileocecal valve and end-to-end, single-layer ileocolic anastomosis; and Group B (n = 7) with resection of the ileocecal valve and end-to-end, single-layer ileocolic anastomosis followed by construction of an artificial sphincter. Intraluminal contents were collected from both groups. The animals were reoperated 20 days after the first procedure, with new collection of intraluminal contents of the ileum and colon. From the samples collected, DNA was extracted for PCR-DGGE. The electrophoretic banding patterns generated in the reaction were analyzed for similarities and diversities of the microbiota. The diversity of microorganisms was larger and in more samples when collected from the ileum than from the colon. The group with the neosphincter showed the highest variation in the colon, from 2.11 to 2.93. In three animals from each group w established similarity comparison, and they displayed no similarity with controls. ileocecal resection led to changes in ileal microbiota and, with the creation of new sphincter, the changes were even greater.engRevista do Colégio Brasileiro de Cirurgiõesv.39, n.6, p. 521-528, Nov./Dec. 2012IntestinesAnal canalIleocecal valvePolymerase chain reactionMolecular biologyAnalysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincterinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf510239https://locus.ufv.br//bitstream/123456789/16857/1/artigo.pdf17d42496e77a513aab9b79a5d00d5abbMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/16857/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg7110https://locus.ufv.br//bitstream/123456789/16857/3/artigo.pdf.jpg7e2487c1037aeee4d5428b35bae50b8fMD53123456789/168572018-01-26 22:00:22.017oai:locus.ufv.br:123456789/16857Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-01-27T01:00:22LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter |
title |
Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter |
spellingShingle |
Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter Barra, Ângela Aparecida Intestines Anal canal Ileocecal valve Polymerase chain reaction Molecular biology |
title_short |
Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter |
title_full |
Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter |
title_fullStr |
Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter |
title_full_unstemmed |
Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter |
title_sort |
Analysis of the diversity of the intestinal microbiota of rats subjected to resection of the ileocecalvalve and creation of artificial sphincter |
author |
Barra, Ângela Aparecida |
author_facet |
Barra, Ângela Aparecida Ferreira, Alessandra Barbosa Nagen, Marcelo Freitas, Fernanda de Souza Furtado, Maria Cristina Vasconcelos Silva, Alcino Lázaro da Rena, Cícero de Lima |
author_role |
author |
author2 |
Ferreira, Alessandra Barbosa Nagen, Marcelo Freitas, Fernanda de Souza Furtado, Maria Cristina Vasconcelos Silva, Alcino Lázaro da Rena, Cícero de Lima |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Barra, Ângela Aparecida Ferreira, Alessandra Barbosa Nagen, Marcelo Freitas, Fernanda de Souza Furtado, Maria Cristina Vasconcelos Silva, Alcino Lázaro da Rena, Cícero de Lima |
dc.subject.pt-BR.fl_str_mv |
Intestines Anal canal Ileocecal valve Polymerase chain reaction Molecular biology |
topic |
Intestines Anal canal Ileocecal valve Polymerase chain reaction Molecular biology |
description |
To analyze, through molecular biology, the diversity of the intestinal microbiota before and after resection of the ileocecal junction and reconstruction of intestinal transit with and without the creation of a neosphincter. Fourteen Wistar rats were divided into two groups: Group A (n = 7), submitted to resection of the ileocecal valve and end-to-end, single-layer ileocolic anastomosis; and Group B (n = 7) with resection of the ileocecal valve and end-to-end, single-layer ileocolic anastomosis followed by construction of an artificial sphincter. Intraluminal contents were collected from both groups. The animals were reoperated 20 days after the first procedure, with new collection of intraluminal contents of the ileum and colon. From the samples collected, DNA was extracted for PCR-DGGE. The electrophoretic banding patterns generated in the reaction were analyzed for similarities and diversities of the microbiota. The diversity of microorganisms was larger and in more samples when collected from the ileum than from the colon. The group with the neosphincter showed the highest variation in the colon, from 2.11 to 2.93. In three animals from each group w established similarity comparison, and they displayed no similarity with controls. ileocecal resection led to changes in ileal microbiota and, with the creation of new sphincter, the changes were even greater. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-06-20 |
dc.date.accessioned.fl_str_mv |
2018-01-26T12:11:42Z |
dc.date.available.fl_str_mv |
2018-01-26T12:11:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-69912012000600013 http://www.locus.ufv.br/handle/123456789/16857 |
dc.identifier.issn.none.fl_str_mv |
1809-4546 |
identifier_str_mv |
1809-4546 |
url |
http://dx.doi.org/10.1590/S0100-69912012000600013 http://www.locus.ufv.br/handle/123456789/16857 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v.39, n.6, p. 521-528, Nov./Dec. 2012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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dc.publisher.none.fl_str_mv |
Revista do Colégio Brasileiro de Cirurgiões |
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Revista do Colégio Brasileiro de Cirurgiões |
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LOCUS Repositório Institucional da UFV |
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