Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina

Detalhes bibliográficos
Autor(a) principal: Souza, Luiz Felipe Lourenço de
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5080
Resumo: Bovine herpesvirus type 5 (BoHV-5) is the etiological agent of the bovine meningoencephalitis that presents high morbidity in young bovine. Due to the similarity in the morphology of the virion, cytopathic effect in cell culture and antigenic properties, the BoHV-5 was classified erroneously as BoHV-1 in the past. BoHV-1 is responsible for respiratory and genital diseases, however the disease caused by BoHV-5 presents neurological clinical signs. This virus has been considered a variant of the same agent (BoHV-1) with neuropathogenic characteristics. Comparative studies among isolates of BoHV-5 revealed 97% of proteic similarity. However, the amino-terminal region of the most abundant glycoprotein of the viral envelope, the gC, differs substantially. This glycoprotein is involved in the initial interactions of the virus with the cellular surface besides of presenting as target for antibodies with neutralizing activities. This study aimed at to select an isolate of BoHV-5, among 5 isolates collected in outbreaks in different properties in Brazil, presenting higher antigenicity in vaccinated animals. Vaccines were formulated using isolates ISO9898292, SV507, SV163, 1807 and EVI145 and administered to 5 groups of 10 sheeps, which have received two intramuscular doses on days 0 and 21. Collections of blood were accomplished for analysis of production of antibodies by virus-neutralization and attendance of possible clinical signs to the 63rd day after the first vaccination. Antibodies titers averages ranged from 6,85 and 14,92 on the day 0 before the first vaccination. Antibody peak were on day 14, ranged from 4,50 (isolated ISO9898292) to 138,63 (isolated SV163). A second peak was observed 14 days after the booster ranged from 7,77 (ISO9898292) to 1017,54 (1807). In the 42nd day after the booster, it was observed titers variation from 3,84 (ISO9898292) to 305,97 (1807). The discrepancy among titers of antibody of each group of animals suggests a smaller antigenicity of isolated ISO9898292 in relation to the others, demonstrating a possible variation among the isolates, what results in antigenic differences. All isolates, with exception to ISO9898292, were effective in the induction of antibodies.
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spelling Souza, Luiz Felipe Lourenço dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4127039H6Lamêgo, Márcia Rogéria de Almeidahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4Moraes, Mauro Pireshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723763J8Moreira, Maria Aparecida Scatamburlohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797678J6Pinto, Paulo Sérgio de Arrudahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793537Y3Paula, Sérgio Oliveira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P42015-03-26T13:47:01Z2007-02-072015-03-26T13:47:01Z2006-07-28SOUZA, Luiz Felipe Lourenço de. Comparison of Bovine herpesvirus type 5 (BoHV-5) isolates as vaccine candidates. 2006. 54 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.http://locus.ufv.br/handle/123456789/5080Bovine herpesvirus type 5 (BoHV-5) is the etiological agent of the bovine meningoencephalitis that presents high morbidity in young bovine. Due to the similarity in the morphology of the virion, cytopathic effect in cell culture and antigenic properties, the BoHV-5 was classified erroneously as BoHV-1 in the past. BoHV-1 is responsible for respiratory and genital diseases, however the disease caused by BoHV-5 presents neurological clinical signs. This virus has been considered a variant of the same agent (BoHV-1) with neuropathogenic characteristics. Comparative studies among isolates of BoHV-5 revealed 97% of proteic similarity. However, the amino-terminal region of the most abundant glycoprotein of the viral envelope, the gC, differs substantially. This glycoprotein is involved in the initial interactions of the virus with the cellular surface besides of presenting as target for antibodies with neutralizing activities. This study aimed at to select an isolate of BoHV-5, among 5 isolates collected in outbreaks in different properties in Brazil, presenting higher antigenicity in vaccinated animals. Vaccines were formulated using isolates ISO9898292, SV507, SV163, 1807 and EVI145 and administered to 5 groups of 10 sheeps, which have received two intramuscular doses on days 0 and 21. Collections of blood were accomplished for analysis of production of antibodies by virus-neutralization and attendance of possible clinical signs to the 63rd day after the first vaccination. Antibodies titers averages ranged from 6,85 and 14,92 on the day 0 before the first vaccination. Antibody peak were on day 14, ranged from 4,50 (isolated ISO9898292) to 138,63 (isolated SV163). A second peak was observed 14 days after the booster ranged from 7,77 (ISO9898292) to 1017,54 (1807). In the 42nd day after the booster, it was observed titers variation from 3,84 (ISO9898292) to 305,97 (1807). The discrepancy among titers of antibody of each group of animals suggests a smaller antigenicity of isolated ISO9898292 in relation to the others, demonstrating a possible variation among the isolates, what results in antigenic differences. All isolates, with exception to ISO9898292, were effective in the induction of antibodies.O herpesvírus bovino tipo 5 (BoHV-5) é o agente etiológico da menigoencefalite bovina que apresenta alta morbidade em bovinos jovens. Devido à similaridade na morfologia do virion, efeito citopático na cultura de células e propriedades antigênicas, o BoHV-5 foi classificado erroneamente como herpesvírus bovino tipo 1 (BoHV-1). Este vírus é responsável por doenças respiratórias e genitais, porém a doença causada pelo BoHV-5 apresenta sinais clínicos neurológicos, com isso o vírus foi considerado uma variante do mesmo agente com características de neuropatogenicidade. Estudos comparativos entre isolados de BoHV-5 revelaram 97% de similaridade protéica. No entanto, a região amino terminal da glicoproteína mais abundante do envelope viral, a gC, difere substancialmente. Esta glicoproteína está envolvida nas interações iniciais do vírus com a superfície celular, além de se apresentar como alvo para anticorpos com atividades neutralizantes. Este estudo objetivou selecionar um isolado do vírus BoHV-5, entre 5 isolados coletados em surtos da doença em diferentes propriedades, que confira maior antigenicidade em animais vacinados. As vacinas foram formuladas com os isolados ISO9898292, SV507, SV163, 1807 e EVI145 e administradas a 5 grupos de 10 ovelhas, as quais receberam duas doses vacinais por via intramuscular nos dias 0 e 21. Foram realizadas coletas de sangue para análise de produção de anticorpos por virusneutralização e acompanhamento de possíveis sinais clínicos até o 63º dia após a primo-vacinação. Foram observados dois picos na curva de anticorpos, o primeiro no dia 14, após a vacinação, onde os títulos de anticorpos variaram entre 4,50 (isolado ISO9898292) a 138,62 (isolado SV163). O segundo pico foi observado 14 dias após a revacinação onde os títulos variaram entre 7,77 (ISO9898292) a 1017,54 (1807). No 42º dia após a revacinação observou-se variação de título entre 3,84 (ISO9898292) a 305,97 (1807). A discrepância entre as médias de título de anticorpo de cada grupo de animais sugere uma menor antigenicidade do isolado ISO9898292 em relação aos demais, demonstrando uma possível variação entre os isolados, o que resulta em diferenças antigênicas. Todos os isolados, com exceção ao ISO9898292, mostraram-se eficazes na indução de anticorpos.application/pdfporUniversidade Federal de ViçosaMestrado em Medicina VeterináriaUFVBRBiotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. deHerpesvírus bovinoBoHV-5Meningoencefalite bovinaVacina inativadaSoroneutralizaçãoBovine herpesvirusBoHV-5Bovine meningoencephalitisVaccineVirus-neutralizationCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVAComparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacinaComparison of Bovine herpesvirus type 5 (BoHV-5) isolates as vaccine candidatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf258964https://locus.ufv.br//bitstream/123456789/5080/1/texto%20completo.pdf135e6be4766beec15d819dd30575f517MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain88743https://locus.ufv.br//bitstream/123456789/5080/2/texto%20completo.pdf.txt91e875ccb70d471cae608dc59f8e4f8bMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3631https://locus.ufv.br//bitstream/123456789/5080/3/texto%20completo.pdf.jpg43e3e36ad4dc53c0007d3b8db5943d94MD53123456789/50802016-04-11 23:07:56.811oai:locus.ufv.br:123456789/5080Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-12T02:07:56LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina
dc.title.alternative.eng.fl_str_mv Comparison of Bovine herpesvirus type 5 (BoHV-5) isolates as vaccine candidates
title Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina
spellingShingle Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina
Souza, Luiz Felipe Lourenço de
Herpesvírus bovino
BoHV-5
Meningoencefalite bovina
Vacina inativada
Soroneutralização
Bovine herpesvirus
BoHV-5
Bovine meningoencephalitis
Vaccine
Virus-neutralization
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA
title_short Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina
title_full Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina
title_fullStr Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina
title_full_unstemmed Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina
title_sort Comparação de isolados de Herpesvírus bovino tipo 5 (BoHV-5) como candidatos à vacina
author Souza, Luiz Felipe Lourenço de
author_facet Souza, Luiz Felipe Lourenço de
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4127039H6
dc.contributor.author.fl_str_mv Souza, Luiz Felipe Lourenço de
dc.contributor.advisor-co1.fl_str_mv Lamêgo, Márcia Rogéria de Almeida
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4
dc.contributor.advisor1.fl_str_mv Moraes, Mauro Pires
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723763J8
dc.contributor.referee1.fl_str_mv Moreira, Maria Aparecida Scatamburlo
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797678J6
dc.contributor.referee2.fl_str_mv Pinto, Paulo Sérgio de Arruda
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793537Y3
dc.contributor.referee3.fl_str_mv Paula, Sérgio Oliveira de
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P4
contributor_str_mv Lamêgo, Márcia Rogéria de Almeida
Moraes, Mauro Pires
Moreira, Maria Aparecida Scatamburlo
Pinto, Paulo Sérgio de Arruda
Paula, Sérgio Oliveira de
dc.subject.por.fl_str_mv Herpesvírus bovino
BoHV-5
Meningoencefalite bovina
Vacina inativada
Soroneutralização
topic Herpesvírus bovino
BoHV-5
Meningoencefalite bovina
Vacina inativada
Soroneutralização
Bovine herpesvirus
BoHV-5
Bovine meningoencephalitis
Vaccine
Virus-neutralization
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA
dc.subject.eng.fl_str_mv Bovine herpesvirus
BoHV-5
Bovine meningoencephalitis
Vaccine
Virus-neutralization
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA
description Bovine herpesvirus type 5 (BoHV-5) is the etiological agent of the bovine meningoencephalitis that presents high morbidity in young bovine. Due to the similarity in the morphology of the virion, cytopathic effect in cell culture and antigenic properties, the BoHV-5 was classified erroneously as BoHV-1 in the past. BoHV-1 is responsible for respiratory and genital diseases, however the disease caused by BoHV-5 presents neurological clinical signs. This virus has been considered a variant of the same agent (BoHV-1) with neuropathogenic characteristics. Comparative studies among isolates of BoHV-5 revealed 97% of proteic similarity. However, the amino-terminal region of the most abundant glycoprotein of the viral envelope, the gC, differs substantially. This glycoprotein is involved in the initial interactions of the virus with the cellular surface besides of presenting as target for antibodies with neutralizing activities. This study aimed at to select an isolate of BoHV-5, among 5 isolates collected in outbreaks in different properties in Brazil, presenting higher antigenicity in vaccinated animals. Vaccines were formulated using isolates ISO9898292, SV507, SV163, 1807 and EVI145 and administered to 5 groups of 10 sheeps, which have received two intramuscular doses on days 0 and 21. Collections of blood were accomplished for analysis of production of antibodies by virus-neutralization and attendance of possible clinical signs to the 63rd day after the first vaccination. Antibodies titers averages ranged from 6,85 and 14,92 on the day 0 before the first vaccination. Antibody peak were on day 14, ranged from 4,50 (isolated ISO9898292) to 138,63 (isolated SV163). A second peak was observed 14 days after the booster ranged from 7,77 (ISO9898292) to 1017,54 (1807). In the 42nd day after the booster, it was observed titers variation from 3,84 (ISO9898292) to 305,97 (1807). The discrepancy among titers of antibody of each group of animals suggests a smaller antigenicity of isolated ISO9898292 in relation to the others, demonstrating a possible variation among the isolates, what results in antigenic differences. All isolates, with exception to ISO9898292, were effective in the induction of antibodies.
publishDate 2006
dc.date.issued.fl_str_mv 2006-07-28
dc.date.available.fl_str_mv 2007-02-07
2015-03-26T13:47:01Z
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dc.identifier.citation.fl_str_mv SOUZA, Luiz Felipe Lourenço de. Comparison of Bovine herpesvirus type 5 (BoHV-5) isolates as vaccine candidates. 2006. 54 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.
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identifier_str_mv SOUZA, Luiz Felipe Lourenço de. Comparison of Bovine herpesvirus type 5 (BoHV-5) isolates as vaccine candidates. 2006. 54 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.
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