Agrobacterium-mediated genetic transformation of a tropical elite maize line

Detalhes bibliográficos
Autor(a) principal: Souza, Rafaeli Aparecida Vieira de
Data de Publicação: 2016
Outros Autores: Alves, Meire de Cássia, Carneiro, Newton Portilho, Barros, Beatriz de Almeida, Borém, Aluízio, Carneiro, Andréa Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/1984-70332017v17n2a20
http://www.locus.ufv.br/handle/123456789/18570
Resumo: O artigo não contém resumo em português
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spelling Souza, Rafaeli Aparecida Vieira deAlves, Meire de CássiaCarneiro, Newton PortilhoBarros, Beatriz de AlmeidaBorém, AluízioCarneiro, Andréa Almeida2018-03-28T14:08:00Z2018-03-28T14:08:00Z2016-12-0119847033http://dx.doi.org/10.1590/1984-70332017v17n2a20http://www.locus.ufv.br/handle/123456789/18570O artigo não contém resumo em portuguêsThe efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% - half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens.engCrop Breeding and Applied Biotechnologyv. 17, n. 2, p. 133-140, Junho 2017Immature embryosSalt concentrationSomatic embryogenesisZea maysAgrobacterium-mediated genetic transformation of a tropical elite maize lineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf626451https://locus.ufv.br//bitstream/123456789/18570/1/artigo.pdf730f2bc0d291e108b8f1d14706a00c6cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18570/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg4635https://locus.ufv.br//bitstream/123456789/18570/3/artigo.pdf.jpg21bd8038d189a26cab57e9ccd4ecbb35MD53123456789/185702018-03-28 23:00:42.469oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-03-29T02:00:42LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Agrobacterium-mediated genetic transformation of a tropical elite maize line
title Agrobacterium-mediated genetic transformation of a tropical elite maize line
spellingShingle Agrobacterium-mediated genetic transformation of a tropical elite maize line
Souza, Rafaeli Aparecida Vieira de
Immature embryos
Salt concentration
Somatic embryogenesis
Zea mays
title_short Agrobacterium-mediated genetic transformation of a tropical elite maize line
title_full Agrobacterium-mediated genetic transformation of a tropical elite maize line
title_fullStr Agrobacterium-mediated genetic transformation of a tropical elite maize line
title_full_unstemmed Agrobacterium-mediated genetic transformation of a tropical elite maize line
title_sort Agrobacterium-mediated genetic transformation of a tropical elite maize line
author Souza, Rafaeli Aparecida Vieira de
author_facet Souza, Rafaeli Aparecida Vieira de
Alves, Meire de Cássia
Carneiro, Newton Portilho
Barros, Beatriz de Almeida
Borém, Aluízio
Carneiro, Andréa Almeida
author_role author
author2 Alves, Meire de Cássia
Carneiro, Newton Portilho
Barros, Beatriz de Almeida
Borém, Aluízio
Carneiro, Andréa Almeida
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Rafaeli Aparecida Vieira de
Alves, Meire de Cássia
Carneiro, Newton Portilho
Barros, Beatriz de Almeida
Borém, Aluízio
Carneiro, Andréa Almeida
dc.subject.pt-BR.fl_str_mv Immature embryos
Salt concentration
Somatic embryogenesis
Zea mays
topic Immature embryos
Salt concentration
Somatic embryogenesis
Zea mays
description O artigo não contém resumo em português
publishDate 2016
dc.date.issued.fl_str_mv 2016-12-01
dc.date.accessioned.fl_str_mv 2018-03-28T14:08:00Z
dc.date.available.fl_str_mv 2018-03-28T14:08:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1984-70332017v17n2a20
http://www.locus.ufv.br/handle/123456789/18570
dc.identifier.issn.none.fl_str_mv 19847033
identifier_str_mv 19847033
url http://dx.doi.org/10.1590/1984-70332017v17n2a20
http://www.locus.ufv.br/handle/123456789/18570
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 17, n. 2, p. 133-140, Junho 2017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
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