Avaliação da eficiência de sabonetes com triclosan sobre suspensões bacterianas de Escherichia coli e Staphylococcus aureus aplicadas sobre a superfície das mãos de voluntários

• Autor(a) principal: Soares, Monica Porto Maia
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2926
• topic: Manipuladores; Triclosan; Higienização de mãos; Handlers; Triclosan; Washing hands; CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS

Tests for evaluating antiseptic products are essential for proving efficiency of different products on the market as well as being a safe choice in the products used for hygiene procedures in several areas. For this study, we analyzed five brands of antiseptic liquid soaps containing triclosan at a concentration of 0,5 % (m/m) and a neutral liquid soap to assess the real need to use products with antimicrobial activity in industries, hospitals and homes. The following steps were performed: I) evaluation of physico-chemical characteristics of soaps; ii) evaluation of the level of triclosan in the antiseptic soaps; III) evaluation of the bacteriostatic/bactericidal activity of triclosan 0,5 % (m/v) on bacterial suspensions of Escherichia coli and Staphylococcus aureus; IV) evaluation of the efficiency of the soaps on bacterial suspensions of E. coli and S. aureus by testing inhibition of microbial growth by agar diffusion; v) evaluation of the efficiency of the soaps on bacterial suspensions of E. coli and S. aureus in real conditions, using volunteers, under standard conditions. The values found for both viscosity and for the interfacial tension of the soap differed greatly, ranging from 268 to 793 centistokes and 14,42 to 26,89 mN/m, respectively. This is probably due to their different formulations. The pH varied from 5,95 to 7,07, were only the neutral soap approached the physiological pH of the skin (4,2 to 5,9). The density varied little among soaps, from 1,0131 to 1,0233, despite being different formulations. Only one of the antiseptic soaps presented triclosan content below specified on the label. In this experiment conditions, triclosan 0,5 % (m/v) possessed no bacteriostatic effect, only bactericidal effect on micro-organisms E. coli and S. aureus. The gram-negative bacterium E. coli was less sensitive to the action of soaps than the gram- positive S. aureus on the microbial inhibition multiplication test by agar diffusion. This is probably due to the differences in cell wall of both bacteria. In the test using volunteers there was no significant difference (p> 0,05) among soaps (1 to 6) for both bacteria under study, indicating that the use of liquid soaps containing triclosan is not necessary in the process of washing hands. The recoverability of pathogenic micro-organisms from hands of volunteers was different in the adhesion test. The number of E. coli reached average 4,36 log CFU/hand while for S. aureus that value was 5,66 log CFU/hand. Whereas were added between 8-9 log CFU/mL to the hands of volunteers and that the procedure for removal of these micro-organisms was very well standardized, it can be stated that the E. coli was the most difficult to be removed, ie the more strongly adhered to hands.