Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura

Detalhes bibliográficos
Autor(a) principal: Faria, Bárbara Müller Salomão de
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4791
Resumo: The present work reports results from the research developed at the "International consortium for the sequencing of the genome and transcriptome of the common bean (Phaseolus vulgaris)" (Prosul/CNPq and CYTED) project conducted at Embrapa Rice and Beans (CNPAF) and the Federal University of Viçosa (UFV), which is composed of two distinct subprojects: Sequencing and characterization of the BACs-ends and Identification and development of SNPs markers in common bean . The dissert were structured in two chapters, each derived from their respective subprojects, containing the objectives and the main results presented below: Chapter 1- With the intention of amplifying and deepening the understanding about the P. vulgaris genome structure and composition, the goal of this study was to generate BAC-end sequences (BESs) from one BAC (Bacterial Artificial Chromosome) library and analyze the sequences for the presence of SSRs and genomic annotation. In total, 52,270 BESs were generated and processed equaling 32 Mbp (6.5%) of the P. vulgaris genome, with 39% of GC content. A total of 3,789 BES-SSRs were found, one for every 8.36 kb. Of these, 2,000 SSRs were appropriated for the development of molecular markers, of these 194 were evaluated and 40 were characterized for genetic and operational aspects. Of the 52,270 BESs, approximately, 2% contained sequences that encode transcription factors and 3% contained transposable elements. Through the comparison with the NCBI non-redundant protein database, it was possible to identify putative functions for 24,321 BESs, accounting for 46.53% of the total sequences analyzed. According to Gene Ontology (GO) terms functional categories were assigned to 19,363 BESs involved in biological process (52%), molecular function (65%) and cellular component (22%). This study allowed us to successfully identify BES-SSRs, highly polymorphic, searching for SSRs motifs of tri- to hexanucleotides, adding appropriate genetic features and with the potential to be used in the common bean genetic breeding programs. Additionally, the generated and processed BESs were integrated into the international project of common bean genome sequencing assisting in the composition and assembly of the genome. Chapter 2- The objectives of this study were to evaluate and compare the informativeness of 58 SSRs (24 SSRs-di dinucleotide microsatellites and 34 BES-SSRs tri- to hexanucleotide microsatellites) and 345 SNPs, as suited tools to P. vulgaris breeding programs. A germplasm set of 88 genotypes compounded of 55 breeding material and 33 landraces was evaluated, including eight biparental combinations derived from inter and intra-gene pool crosses. The SSRs-di displayed higher average of alleles/locus (9.916) and gene diversity (72.1%), exhibiting a superior capacity to distinguish the whole group of genotypes. Fourteen SSRs, out of the 58, with a considerable number of private alleles (over 14.93/locus) and high levels of gene diversity (84%), discriminated all 88 genotypes. The polymorphic SNPs, among inter (78.2%) and intra-gene pool (17.7%) combinations, were evaluated concerning their employment in genetic mapping. The approaches utilized to infer the genetic population structure presented correspondent results among all classes of markers, differentiating the genotypes within Andean and Mesoamerican gene pools. The SNPs, however, evidenced a superior (K = 2, FST = 0.895) competence to differentiate between the two gene pools. Furthermore, the SSRs-di distinguished, within Mesoamerican gene pool, the breeding material and the landraces germplasm (K = 3). Despite the high levels of linkage disequilibrium for both SSRs and SNPs, the latter yielded higher levels (84.92%). The linkage disequilibrium levels dropped when Andean and Mesoamerican genotypes were analyzed separately. The SSRs and SNPs distribution in P. vulgaris genome was abundant and random. Concerning the breeding program, SSRs and SNPs genotyping panels are now available, allowing the germplasm origin to be disclosed. In addition, a group of polymorphic SNPs, between several biparental populations, presents itself as an extension of such technology in the development of high-density genetic maps, with a substantial marker overlapping through crosses. This study contributes for the integration of genomic tools within breeding programs, connecting feasible and low cost techniques with efficient/wide genome sampling of the common bean.
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spelling Faria, Bárbara Müller Salomão dehttp://lattes.cnpq.br/0952987528921052Vianello, Rosana Pereirahttp://lattes.cnpq.br/4523999698824309Moreira, Maurílio Alveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796105P2Barros, Everaldo Gonçalves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6Novaes, Evandrohttp://lattes.cnpq.br/0568272239145336Souza, Thiago Lívio Pessoa Oliveira dehttp://lattes.cnpq.br/96501833087791432015-03-26T13:42:30Z2014-01-212015-03-26T13:42:30Z2013-07-29FARIA, Bárbara Müller Salomão de. Genomic resources characterization of the common bean (Phaseolus vulgaris L.) for the development of useful SSRs and SNPs to the genetic and breeding study of the crop. 2013. 133 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2013.http://locus.ufv.br/handle/123456789/4791The present work reports results from the research developed at the "International consortium for the sequencing of the genome and transcriptome of the common bean (Phaseolus vulgaris)" (Prosul/CNPq and CYTED) project conducted at Embrapa Rice and Beans (CNPAF) and the Federal University of Viçosa (UFV), which is composed of two distinct subprojects: Sequencing and characterization of the BACs-ends and Identification and development of SNPs markers in common bean . The dissert were structured in two chapters, each derived from their respective subprojects, containing the objectives and the main results presented below: Chapter 1- With the intention of amplifying and deepening the understanding about the P. vulgaris genome structure and composition, the goal of this study was to generate BAC-end sequences (BESs) from one BAC (Bacterial Artificial Chromosome) library and analyze the sequences for the presence of SSRs and genomic annotation. In total, 52,270 BESs were generated and processed equaling 32 Mbp (6.5%) of the P. vulgaris genome, with 39% of GC content. A total of 3,789 BES-SSRs were found, one for every 8.36 kb. Of these, 2,000 SSRs were appropriated for the development of molecular markers, of these 194 were evaluated and 40 were characterized for genetic and operational aspects. Of the 52,270 BESs, approximately, 2% contained sequences that encode transcription factors and 3% contained transposable elements. Through the comparison with the NCBI non-redundant protein database, it was possible to identify putative functions for 24,321 BESs, accounting for 46.53% of the total sequences analyzed. According to Gene Ontology (GO) terms functional categories were assigned to 19,363 BESs involved in biological process (52%), molecular function (65%) and cellular component (22%). This study allowed us to successfully identify BES-SSRs, highly polymorphic, searching for SSRs motifs of tri- to hexanucleotides, adding appropriate genetic features and with the potential to be used in the common bean genetic breeding programs. Additionally, the generated and processed BESs were integrated into the international project of common bean genome sequencing assisting in the composition and assembly of the genome. Chapter 2- The objectives of this study were to evaluate and compare the informativeness of 58 SSRs (24 SSRs-di dinucleotide microsatellites and 34 BES-SSRs tri- to hexanucleotide microsatellites) and 345 SNPs, as suited tools to P. vulgaris breeding programs. A germplasm set of 88 genotypes compounded of 55 breeding material and 33 landraces was evaluated, including eight biparental combinations derived from inter and intra-gene pool crosses. The SSRs-di displayed higher average of alleles/locus (9.916) and gene diversity (72.1%), exhibiting a superior capacity to distinguish the whole group of genotypes. Fourteen SSRs, out of the 58, with a considerable number of private alleles (over 14.93/locus) and high levels of gene diversity (84%), discriminated all 88 genotypes. The polymorphic SNPs, among inter (78.2%) and intra-gene pool (17.7%) combinations, were evaluated concerning their employment in genetic mapping. The approaches utilized to infer the genetic population structure presented correspondent results among all classes of markers, differentiating the genotypes within Andean and Mesoamerican gene pools. The SNPs, however, evidenced a superior (K = 2, FST = 0.895) competence to differentiate between the two gene pools. Furthermore, the SSRs-di distinguished, within Mesoamerican gene pool, the breeding material and the landraces germplasm (K = 3). Despite the high levels of linkage disequilibrium for both SSRs and SNPs, the latter yielded higher levels (84.92%). The linkage disequilibrium levels dropped when Andean and Mesoamerican genotypes were analyzed separately. The SSRs and SNPs distribution in P. vulgaris genome was abundant and random. Concerning the breeding program, SSRs and SNPs genotyping panels are now available, allowing the germplasm origin to be disclosed. In addition, a group of polymorphic SNPs, between several biparental populations, presents itself as an extension of such technology in the development of high-density genetic maps, with a substantial marker overlapping through crosses. This study contributes for the integration of genomic tools within breeding programs, connecting feasible and low cost techniques with efficient/wide genome sampling of the common bean.Este trabalho apresenta resultados de pesquisas desenvolvidas no âmbito do Projeto Consórcio internacional para o sequenciamento do genoma e do transcriptoma do feijoeiro comum (Phaseolus vulgaris) (Prosul/CNPq e CYTED) conduzido na Embrapa Arroz e Feijão (CNPAF) e na Universidade Federal de Viçosa (UFV), sendo este composto por dois subprojetos distintos: Sequenciamento e caracterização das pontas de BACs e Identificação e desenvolvimento de marcadores SNPs em feijoeiro comum . A dissertação foi estruturada em dois capítulos, cada um derivado de um subprojeto, contendo os objetivos e os principais resultados apresentados a seguir: Capítulo 1- Com o intuito de ampliar e aprofundar o conhecimento sobre a estrutura e composição do genoma de P. vulgaris este estudo teve como objetivo sequenciar uma biblioteca BAC (Bacterial Artificial Chromosome) a partir de suas extremidades (BESs BAC-end sequences) e analisar as sequências quanto à presença de SSRs e a anotação genômica. Ao todo, 52.270 BESs foram geradas e processadas equivalendo a 32 Mpb (6,5%) do genoma de P. vulgaris, com conteúdo de GC estimado em 39%. Foram encontrados um total de 3.789 BES-SSRs, um a cada 8,36 kb. Destes, 2.000 foram adequados para o desenvolvimento de marcadores moleculares, dos quais 194 foram avaliados e 40 deles foram caracterizados quanto a aspectos genéticos operacionais. Das 52.270 BESs, aproximadamente 2% continham sequências que codificavam fatores de transcrição e 3% continham elementos transponíveis. Através da comparação com o banco de dados não-redundante de proteínas do NCBI, foi possível identificar funções putativas para 24.321 BESs, contabilizando 46,53% do total de sequências analisadas. Com base nos termos do Gene Ontology (GO), foram atribuídas categorias funcionais a 19.363 BESs inseridas em processos biológicos (52%), função molecular (65%) e componente celular (22%). Este estudo permitiu identificar com sucesso BES-SSRs altamente polimórficos, buscando motivos SSRs de tri- a hexanucleotídeos, agregando características genéticas adequadas e com potencial de serem utilizados no programa de melhoramento genético de feijoeiro comum. Adicionalmente, as BESs geradas e processadas foram integradas ao projeto internacional de sequenciamento do genoma de feijoeiro comum auxiliando na composição e montagem do mesmo. Capítulo 2- O objetivo desse estudo foi avaliar e comparar o poder de informação genética de um grupo de 58 marcadores SSRs (24 SSRs-di microssatélites dinucleotídeo e 34 BES-SSRs microssatélites tri- a hexanucleoídeo) e 345 SNPs para aplicações no melhoramento genético de P. vulgaris. Foram avaliados 88 genótipos representativos de 55 acessos melhorados e 33 variedades tradicionais, incluindo oito combinações biparentais composta por cruzamentos inter- e intra-pool gênico. Os SSRs-di apresentaram maior média de alelos por loco gênico (9,916) e revelaram maior diversidade genética média (72,1%), sendo o grupo de marcadores com o maior poder de discriminação genética entre indivíduos. Entre os 58 SSRs, 14 com uma elevada média de alelos privativos (14,93/loco) e diversidade genética (84%) possibilitaram a discriminação individual dos 88 genótipos. Os SNPs polimórficos entre cruzamentos biparentais, intra- (17,7%) e inter-pool gênico (78,2%), foram avaliados quanto à aplicação prática no mapeamento genético. As abordagens utilizadas para inferir a estrutura genética populacional apresentaram resultados similares entre os grupos de marcadores, discriminando os genótipos nos pools gênicos Mesoamericano e Andino, com a maior diferenciação genética (K = 2, FST = 0,895) apresentada pelos SNPs. Os SSRs- di possibilitaram discriminar dentro do pool gênico Mesoamenricano o germoplasma melhorado e tradicional (K = 3). O desequilíbrio de ligação testado para todos os marcadores foi elevado, sendo maior para os SNPs (84,92%), reduzindo significativamente quando avaliado separadamente para os genótipos Andinos e Mesoamericanos. A distribuição dos SSRs e SNPs foi ampla e aleatória em todo o genoma de P. vulgaris. Em termos de resultados práticos para o programa de melhoramento, neste estudo foram derivados painéis de genotipagem operacionais baseado em SSRs e SNPs com alto e eficiente poder de discriminação do germoplasma por origem. Adicionalmente, um conjunto de SNPs polimórficos entre diversas populações biparentais representa uma aplicação adicional dessa tecnologia para geração de mapas genéticos de alta densidade compreendendo uma proporção substancial de marcadores compartilhados entre cruzamentos. Esse estudo contribui para a integração crescente da genômica nos programas de melhoramento, aliando metodologias de genotipagem acessíveis e a baixos custos que permitem amostrar de modo eficiente e/ou amplo o genoma de feijoeiro comum.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Genética e MelhoramentoUFVBRGenética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; MeLeguminosasGenomaMarcadores molecularesBAC-end sequencesDiversidade genéticaEstrutura populacionalLegumesGenomeMolecular markersBAC-end sequencesGenetic diversityPopulation structureCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETALCaracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da culturaGenomic resources characterization of the common bean (Phaseolus vulgaris L.) for the development of useful SSRs and SNPs to the genetic and breeding study of the cropinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf3476957https://locus.ufv.br//bitstream/123456789/4791/1/texto%20completo.pdf7b79218599a11932aefca465d11aafc6MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain270309https://locus.ufv.br//bitstream/123456789/4791/2/texto%20completo.pdf.txtc10d6e3d57b15e0ace899df8740f4c1aMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3711https://locus.ufv.br//bitstream/123456789/4791/3/texto%20completo.pdf.jpgdcf363c726a010cf549236e061aa546bMD53123456789/47912016-04-10 23:16:17.402oai:locus.ufv.br:123456789/4791Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:16:17LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura
dc.title.alternative.eng.fl_str_mv Genomic resources characterization of the common bean (Phaseolus vulgaris L.) for the development of useful SSRs and SNPs to the genetic and breeding study of the crop
title Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura
spellingShingle Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura
Faria, Bárbara Müller Salomão de
Leguminosas
Genoma
Marcadores moleculares
BAC-end sequences
Diversidade genética
Estrutura populacional
Legumes
Genome
Molecular markers
BAC-end sequences
Genetic diversity
Population structure
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
title_short Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura
title_full Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura
title_fullStr Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura
title_full_unstemmed Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura
title_sort Caracterização de recursos genômicos do feijoeiro comum (Phaseolus vulgaris L.) para o desenvolvimento de SSRs e SNPs úteis ao estudo genético e melhoramento da cultura
author Faria, Bárbara Müller Salomão de
author_facet Faria, Bárbara Müller Salomão de
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/0952987528921052
dc.contributor.author.fl_str_mv Faria, Bárbara Müller Salomão de
dc.contributor.advisor-co1.fl_str_mv Vianello, Rosana Pereira
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/4523999698824309
dc.contributor.advisor-co2.fl_str_mv Moreira, Maurílio Alves
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796105P2
dc.contributor.advisor1.fl_str_mv Barros, Everaldo Gonçalves de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6
dc.contributor.referee1.fl_str_mv Novaes, Evandro
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/0568272239145336
dc.contributor.referee2.fl_str_mv Souza, Thiago Lívio Pessoa Oliveira de
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/9650183308779143
contributor_str_mv Vianello, Rosana Pereira
Moreira, Maurílio Alves
Barros, Everaldo Gonçalves de
Novaes, Evandro
Souza, Thiago Lívio Pessoa Oliveira de
dc.subject.por.fl_str_mv Leguminosas
Genoma
Marcadores moleculares
BAC-end sequences
Diversidade genética
Estrutura populacional
topic Leguminosas
Genoma
Marcadores moleculares
BAC-end sequences
Diversidade genética
Estrutura populacional
Legumes
Genome
Molecular markers
BAC-end sequences
Genetic diversity
Population structure
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
dc.subject.eng.fl_str_mv Legumes
Genome
Molecular markers
BAC-end sequences
Genetic diversity
Population structure
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
description The present work reports results from the research developed at the "International consortium for the sequencing of the genome and transcriptome of the common bean (Phaseolus vulgaris)" (Prosul/CNPq and CYTED) project conducted at Embrapa Rice and Beans (CNPAF) and the Federal University of Viçosa (UFV), which is composed of two distinct subprojects: Sequencing and characterization of the BACs-ends and Identification and development of SNPs markers in common bean . The dissert were structured in two chapters, each derived from their respective subprojects, containing the objectives and the main results presented below: Chapter 1- With the intention of amplifying and deepening the understanding about the P. vulgaris genome structure and composition, the goal of this study was to generate BAC-end sequences (BESs) from one BAC (Bacterial Artificial Chromosome) library and analyze the sequences for the presence of SSRs and genomic annotation. In total, 52,270 BESs were generated and processed equaling 32 Mbp (6.5%) of the P. vulgaris genome, with 39% of GC content. A total of 3,789 BES-SSRs were found, one for every 8.36 kb. Of these, 2,000 SSRs were appropriated for the development of molecular markers, of these 194 were evaluated and 40 were characterized for genetic and operational aspects. Of the 52,270 BESs, approximately, 2% contained sequences that encode transcription factors and 3% contained transposable elements. Through the comparison with the NCBI non-redundant protein database, it was possible to identify putative functions for 24,321 BESs, accounting for 46.53% of the total sequences analyzed. According to Gene Ontology (GO) terms functional categories were assigned to 19,363 BESs involved in biological process (52%), molecular function (65%) and cellular component (22%). This study allowed us to successfully identify BES-SSRs, highly polymorphic, searching for SSRs motifs of tri- to hexanucleotides, adding appropriate genetic features and with the potential to be used in the common bean genetic breeding programs. Additionally, the generated and processed BESs were integrated into the international project of common bean genome sequencing assisting in the composition and assembly of the genome. Chapter 2- The objectives of this study were to evaluate and compare the informativeness of 58 SSRs (24 SSRs-di dinucleotide microsatellites and 34 BES-SSRs tri- to hexanucleotide microsatellites) and 345 SNPs, as suited tools to P. vulgaris breeding programs. A germplasm set of 88 genotypes compounded of 55 breeding material and 33 landraces was evaluated, including eight biparental combinations derived from inter and intra-gene pool crosses. The SSRs-di displayed higher average of alleles/locus (9.916) and gene diversity (72.1%), exhibiting a superior capacity to distinguish the whole group of genotypes. Fourteen SSRs, out of the 58, with a considerable number of private alleles (over 14.93/locus) and high levels of gene diversity (84%), discriminated all 88 genotypes. The polymorphic SNPs, among inter (78.2%) and intra-gene pool (17.7%) combinations, were evaluated concerning their employment in genetic mapping. The approaches utilized to infer the genetic population structure presented correspondent results among all classes of markers, differentiating the genotypes within Andean and Mesoamerican gene pools. The SNPs, however, evidenced a superior (K = 2, FST = 0.895) competence to differentiate between the two gene pools. Furthermore, the SSRs-di distinguished, within Mesoamerican gene pool, the breeding material and the landraces germplasm (K = 3). Despite the high levels of linkage disequilibrium for both SSRs and SNPs, the latter yielded higher levels (84.92%). The linkage disequilibrium levels dropped when Andean and Mesoamerican genotypes were analyzed separately. The SSRs and SNPs distribution in P. vulgaris genome was abundant and random. Concerning the breeding program, SSRs and SNPs genotyping panels are now available, allowing the germplasm origin to be disclosed. In addition, a group of polymorphic SNPs, between several biparental populations, presents itself as an extension of such technology in the development of high-density genetic maps, with a substantial marker overlapping through crosses. This study contributes for the integration of genomic tools within breeding programs, connecting feasible and low cost techniques with efficient/wide genome sampling of the common bean.
publishDate 2013
dc.date.issued.fl_str_mv 2013-07-29
dc.date.available.fl_str_mv 2014-01-21
2015-03-26T13:42:30Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:42:30Z
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dc.identifier.citation.fl_str_mv FARIA, Bárbara Müller Salomão de. Genomic resources characterization of the common bean (Phaseolus vulgaris L.) for the development of useful SSRs and SNPs to the genetic and breeding study of the crop. 2013. 133 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2013.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4791
identifier_str_mv FARIA, Bárbara Müller Salomão de. Genomic resources characterization of the common bean (Phaseolus vulgaris L.) for the development of useful SSRs and SNPs to the genetic and breeding study of the crop. 2013. 133 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2013.
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me
publisher.none.fl_str_mv Universidade Federal de Viçosa
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