Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1002/ar.23196 http://www.locus.ufv.br/handle/123456789/12802 |
Resumo: | To investigate possible morphological changes to the liver tissue of lambaris, Astyanax bimaculatus (Linnaeus, 1758), females were exposed to treatments of sublethal concentrations of the insecticide Thiodan® for 96 hr. Treatments included three sublethal concentrations of 1.15, 2.3, and 5.6 μg L−1 of Thiodan® and a control group without insecticide. The action of Thiodan® at sublethal concentrations did not affect the morphological structure of the liver as a whole, but changes in isolated locations of the hepatic parenchyma were observed. Glycogen depletion, nuclear and cytoplasmic deformation, nuclear and cytoplasmic hypertrophy, hyperemia, and cellular degeneration in liver cells at the different concentrations studied were recorded. These observed changes in the livers were greater in groups exposed to Thiodan® in comparison to the control group. Furthermore, there was a change in the diameter of the nuclei and cytoplasm of hepatocytes in the different treatments. The groups exposed to Thiodan® also exhibited a larger number of hepatocyte nuclei and a reduction in the amount of cytoplasm. We conclude that for the exposure period and concentrations of Thiodan® analyzed, the morphology of hepatic tissue had a cellular adaptive response. |
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Marcon, LucasBazzoli, NiloMounteer, Ann HonorBenjamin, Laércio Dos Anjos2017-11-07T10:27:41Z2017-11-07T10:27:41Z2015-07-161932-8494http://dx.doi.org/10.1002/ar.23196http://www.locus.ufv.br/handle/123456789/12802To investigate possible morphological changes to the liver tissue of lambaris, Astyanax bimaculatus (Linnaeus, 1758), females were exposed to treatments of sublethal concentrations of the insecticide Thiodan® for 96 hr. Treatments included three sublethal concentrations of 1.15, 2.3, and 5.6 μg L−1 of Thiodan® and a control group without insecticide. The action of Thiodan® at sublethal concentrations did not affect the morphological structure of the liver as a whole, but changes in isolated locations of the hepatic parenchyma were observed. Glycogen depletion, nuclear and cytoplasmic deformation, nuclear and cytoplasmic hypertrophy, hyperemia, and cellular degeneration in liver cells at the different concentrations studied were recorded. These observed changes in the livers were greater in groups exposed to Thiodan® in comparison to the control group. Furthermore, there was a change in the diameter of the nuclei and cytoplasm of hepatocytes in the different treatments. The groups exposed to Thiodan® also exhibited a larger number of hepatocyte nuclei and a reduction in the amount of cytoplasm. We conclude that for the exposure period and concentrations of Thiodan® analyzed, the morphology of hepatic tissue had a cellular adaptive response.engThe Anatomical Record298(10), p.1754–1764, July 2015HistologyHepatic tissueHepatocyteTeleost fishHistological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticideinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALMarcon_et_al-2015-The_Anatomical_Record.pdfMarcon_et_al-2015-The_Anatomical_Record.pdftexto completoapplication/pdf455077https://locus.ufv.br//bitstream/123456789/12802/1/Marcon_et_al-2015-The_Anatomical_Record.pdfca797468332e4bd9387c48f71ade7561MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12802/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILMarcon_et_al-2015-The_Anatomical_Record.pdf.jpgMarcon_et_al-2015-The_Anatomical_Record.pdf.jpgIM Thumbnailimage/jpeg4634https://locus.ufv.br//bitstream/123456789/12802/3/Marcon_et_al-2015-The_Anatomical_Record.pdf.jpg88e56b49624d68c915ddde1fadbb3944MD53123456789/128022017-11-07 22:01:01.894oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-08T01:01:01LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide |
title |
Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide |
spellingShingle |
Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide Marcon, Lucas Histology Hepatic tissue Hepatocyte Teleost fish |
title_short |
Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide |
title_full |
Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide |
title_fullStr |
Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide |
title_full_unstemmed |
Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide |
title_sort |
Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide |
author |
Marcon, Lucas |
author_facet |
Marcon, Lucas Bazzoli, Nilo Mounteer, Ann Honor Benjamin, Laércio Dos Anjos |
author_role |
author |
author2 |
Bazzoli, Nilo Mounteer, Ann Honor Benjamin, Laércio Dos Anjos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Marcon, Lucas Bazzoli, Nilo Mounteer, Ann Honor Benjamin, Laércio Dos Anjos |
dc.subject.pt-BR.fl_str_mv |
Histology Hepatic tissue Hepatocyte Teleost fish |
topic |
Histology Hepatic tissue Hepatocyte Teleost fish |
description |
To investigate possible morphological changes to the liver tissue of lambaris, Astyanax bimaculatus (Linnaeus, 1758), females were exposed to treatments of sublethal concentrations of the insecticide Thiodan® for 96 hr. Treatments included three sublethal concentrations of 1.15, 2.3, and 5.6 μg L−1 of Thiodan® and a control group without insecticide. The action of Thiodan® at sublethal concentrations did not affect the morphological structure of the liver as a whole, but changes in isolated locations of the hepatic parenchyma were observed. Glycogen depletion, nuclear and cytoplasmic deformation, nuclear and cytoplasmic hypertrophy, hyperemia, and cellular degeneration in liver cells at the different concentrations studied were recorded. These observed changes in the livers were greater in groups exposed to Thiodan® in comparison to the control group. Furthermore, there was a change in the diameter of the nuclei and cytoplasm of hepatocytes in the different treatments. The groups exposed to Thiodan® also exhibited a larger number of hepatocyte nuclei and a reduction in the amount of cytoplasm. We conclude that for the exposure period and concentrations of Thiodan® analyzed, the morphology of hepatic tissue had a cellular adaptive response. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-07-16 |
dc.date.accessioned.fl_str_mv |
2017-11-07T10:27:41Z |
dc.date.available.fl_str_mv |
2017-11-07T10:27:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/ar.23196 http://www.locus.ufv.br/handle/123456789/12802 |
dc.identifier.issn.none.fl_str_mv |
1932-8494 |
identifier_str_mv |
1932-8494 |
url |
http://dx.doi.org/10.1002/ar.23196 http://www.locus.ufv.br/handle/123456789/12802 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
298(10), p.1754–1764, July 2015 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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The Anatomical Record |
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The Anatomical Record |
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