Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide

Detalhes bibliográficos
Autor(a) principal: Marcon, Lucas
Data de Publicação: 2015
Outros Autores: Bazzoli, Nilo, Mounteer, Ann Honor, Benjamin, Laércio Dos Anjos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1002/ar.23196
http://www.locus.ufv.br/handle/123456789/12802
Resumo: To investigate possible morphological changes to the liver tissue of lambaris, Astyanax bimaculatus (Linnaeus, 1758), females were exposed to treatments of sublethal concentrations of the insecticide Thiodan® for 96 hr. Treatments included three sublethal concentrations of 1.15, 2.3, and 5.6 μg L−1 of Thiodan® and a control group without insecticide. The action of Thiodan® at sublethal concentrations did not affect the morphological structure of the liver as a whole, but changes in isolated locations of the hepatic parenchyma were observed. Glycogen depletion, nuclear and cytoplasmic deformation, nuclear and cytoplasmic hypertrophy, hyperemia, and cellular degeneration in liver cells at the different concentrations studied were recorded. These observed changes in the livers were greater in groups exposed to Thiodan® in comparison to the control group. Furthermore, there was a change in the diameter of the nuclei and cytoplasm of hepatocytes in the different treatments. The groups exposed to Thiodan® also exhibited a larger number of hepatocyte nuclei and a reduction in the amount of cytoplasm. We conclude that for the exposure period and concentrations of Thiodan® analyzed, the morphology of hepatic tissue had a cellular adaptive response.
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spelling Marcon, LucasBazzoli, NiloMounteer, Ann HonorBenjamin, Laércio Dos Anjos2017-11-07T10:27:41Z2017-11-07T10:27:41Z2015-07-161932-8494http://dx.doi.org/10.1002/ar.23196http://www.locus.ufv.br/handle/123456789/12802To investigate possible morphological changes to the liver tissue of lambaris, Astyanax bimaculatus (Linnaeus, 1758), females were exposed to treatments of sublethal concentrations of the insecticide Thiodan® for 96 hr. Treatments included three sublethal concentrations of 1.15, 2.3, and 5.6 μg L−1 of Thiodan® and a control group without insecticide. The action of Thiodan® at sublethal concentrations did not affect the morphological structure of the liver as a whole, but changes in isolated locations of the hepatic parenchyma were observed. Glycogen depletion, nuclear and cytoplasmic deformation, nuclear and cytoplasmic hypertrophy, hyperemia, and cellular degeneration in liver cells at the different concentrations studied were recorded. These observed changes in the livers were greater in groups exposed to Thiodan® in comparison to the control group. Furthermore, there was a change in the diameter of the nuclei and cytoplasm of hepatocytes in the different treatments. The groups exposed to Thiodan® also exhibited a larger number of hepatocyte nuclei and a reduction in the amount of cytoplasm. We conclude that for the exposure period and concentrations of Thiodan® analyzed, the morphology of hepatic tissue had a cellular adaptive response.engThe Anatomical Record298(10), p.1754–1764, July 2015HistologyHepatic tissueHepatocyteTeleost fishHistological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticideinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALMarcon_et_al-2015-The_Anatomical_Record.pdfMarcon_et_al-2015-The_Anatomical_Record.pdftexto completoapplication/pdf455077https://locus.ufv.br//bitstream/123456789/12802/1/Marcon_et_al-2015-The_Anatomical_Record.pdfca797468332e4bd9387c48f71ade7561MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12802/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILMarcon_et_al-2015-The_Anatomical_Record.pdf.jpgMarcon_et_al-2015-The_Anatomical_Record.pdf.jpgIM Thumbnailimage/jpeg4634https://locus.ufv.br//bitstream/123456789/12802/3/Marcon_et_al-2015-The_Anatomical_Record.pdf.jpg88e56b49624d68c915ddde1fadbb3944MD53123456789/128022017-11-07 22:01:01.894oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-08T01:01:01LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
title Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
spellingShingle Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
Marcon, Lucas
Histology
Hepatic tissue
Hepatocyte
Teleost fish
title_short Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
title_full Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
title_fullStr Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
title_full_unstemmed Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
title_sort Histological and histometric evaluation of the liver in Astyanax bimaculatus (Teleostei: Characidae), exposed to different concentrations of an organochlorine insecticide
author Marcon, Lucas
author_facet Marcon, Lucas
Bazzoli, Nilo
Mounteer, Ann Honor
Benjamin, Laércio Dos Anjos
author_role author
author2 Bazzoli, Nilo
Mounteer, Ann Honor
Benjamin, Laércio Dos Anjos
author2_role author
author
author
dc.contributor.author.fl_str_mv Marcon, Lucas
Bazzoli, Nilo
Mounteer, Ann Honor
Benjamin, Laércio Dos Anjos
dc.subject.pt-BR.fl_str_mv Histology
Hepatic tissue
Hepatocyte
Teleost fish
topic Histology
Hepatic tissue
Hepatocyte
Teleost fish
description To investigate possible morphological changes to the liver tissue of lambaris, Astyanax bimaculatus (Linnaeus, 1758), females were exposed to treatments of sublethal concentrations of the insecticide Thiodan® for 96 hr. Treatments included three sublethal concentrations of 1.15, 2.3, and 5.6 μg L−1 of Thiodan® and a control group without insecticide. The action of Thiodan® at sublethal concentrations did not affect the morphological structure of the liver as a whole, but changes in isolated locations of the hepatic parenchyma were observed. Glycogen depletion, nuclear and cytoplasmic deformation, nuclear and cytoplasmic hypertrophy, hyperemia, and cellular degeneration in liver cells at the different concentrations studied were recorded. These observed changes in the livers were greater in groups exposed to Thiodan® in comparison to the control group. Furthermore, there was a change in the diameter of the nuclei and cytoplasm of hepatocytes in the different treatments. The groups exposed to Thiodan® also exhibited a larger number of hepatocyte nuclei and a reduction in the amount of cytoplasm. We conclude that for the exposure period and concentrations of Thiodan® analyzed, the morphology of hepatic tissue had a cellular adaptive response.
publishDate 2015
dc.date.issued.fl_str_mv 2015-07-16
dc.date.accessioned.fl_str_mv 2017-11-07T10:27:41Z
dc.date.available.fl_str_mv 2017-11-07T10:27:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/ar.23196
http://www.locus.ufv.br/handle/123456789/12802
dc.identifier.issn.none.fl_str_mv 1932-8494
identifier_str_mv 1932-8494
url http://dx.doi.org/10.1002/ar.23196
http://www.locus.ufv.br/handle/123456789/12802
dc.language.iso.fl_str_mv eng
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dc.relation.ispartofseries.pt-BR.fl_str_mv 298(10), p.1754–1764, July 2015
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dc.publisher.none.fl_str_mv The Anatomical Record
publisher.none.fl_str_mv The Anatomical Record
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instname:Universidade Federal de Viçosa (UFV)
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