Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection

Detalhes bibliográficos
Autor(a) principal: Alves, Murilo S.
Data de Publicação: 2015
Outros Autores: Soares, Zamira G., Vidigal, Pedro M. P., Barros, Everaldo G., Poddanosqui, Adriana M. P., Aoyagi, Luciano N., Abdelnoor, Ricardo V., Marcelino-Guimarães, Francismar C., Fietto, Luciano G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1007/s10142-015-0445-0
http://www.locus.ufv.br/handle/123456789/19328
Resumo: Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is one of most important diseases in the soybean (Glycine max (L.) Merr.) agribusiness. The identification and characterization of genes related to plant defense responses to fungal infection are essential to develop ASR-resistant plants. In this work, we describe four soybean genes, GmbZIP62, GmbZIP105, GmbZIPE1, and GmbZIPE2, which encode transcription factors containing a basic leucine zipper (bZIP) domain from two divergent classes, and that are responsive to P. pachyrhizi infection. Molecular phylogenetic analyses demonstrated that these genes encode proteins similar to bZIP factors responsive to pathogens. Yeast transactivation assays showed that only GmbZIP62 has strong transactivation activity in yeast. In addition, three of the bZIP transcription factors analyzed were also differentially expressed by plant defense hormones, and all were differentially expressed by fungal attack, indicating that these proteins might participate in response to ASR infection. The results suggested that these bZIP proteins are part of the plant defense response to P. pachyrhizi infection, by regulating the gene expression related to ASR infection responses. These bZIP genes are potential targets to obtain new soybean genotypes resistant to ASR.
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spelling Alves, Murilo S.Soares, Zamira G.Vidigal, Pedro M. P.Barros, Everaldo G.Poddanosqui, Adriana M. P.Aoyagi, Luciano N.Abdelnoor, Ricardo V.Marcelino-Guimarães, Francismar C.Fietto, Luciano G.2018-05-04T13:22:18Z2018-05-04T13:22:18Z2015-05-2714387948https://doi.org/10.1007/s10142-015-0445-0http://www.locus.ufv.br/handle/123456789/19328Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is one of most important diseases in the soybean (Glycine max (L.) Merr.) agribusiness. The identification and characterization of genes related to plant defense responses to fungal infection are essential to develop ASR-resistant plants. In this work, we describe four soybean genes, GmbZIP62, GmbZIP105, GmbZIPE1, and GmbZIPE2, which encode transcription factors containing a basic leucine zipper (bZIP) domain from two divergent classes, and that are responsive to P. pachyrhizi infection. Molecular phylogenetic analyses demonstrated that these genes encode proteins similar to bZIP factors responsive to pathogens. Yeast transactivation assays showed that only GmbZIP62 has strong transactivation activity in yeast. In addition, three of the bZIP transcription factors analyzed were also differentially expressed by plant defense hormones, and all were differentially expressed by fungal attack, indicating that these proteins might participate in response to ASR infection. The results suggested that these bZIP proteins are part of the plant defense response to P. pachyrhizi infection, by regulating the gene expression related to ASR infection responses. These bZIP genes are potential targets to obtain new soybean genotypes resistant to ASR.engFunctional & Integrative Genomicsv. 15, Issue 6, p. 685–696, November 2015Springer-Verlag Berlin Heidelberginfo:eu-repo/semantics/openAccessBiotic stressSignal transductionPlant defenseAsian soybean rustTranscriptional controlDifferential expression of four soybean bZIP genes during Phakopsora pachyrhizi infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf806221https://locus.ufv.br//bitstream/123456789/19328/1/artigo.pdf184ca776daf40e500bc16584b6213bc4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19328/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5065https://locus.ufv.br//bitstream/123456789/19328/3/artigo.pdf.jpg2a9a10bee2dfd6ee80e4840a20f520c7MD53123456789/193282018-05-04 23:00:31.534oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-05T02:00:31LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection
title Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection
spellingShingle Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection
Alves, Murilo S.
Biotic stress
Signal transduction
Plant defense
Asian soybean rust
Transcriptional control
title_short Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection
title_full Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection
title_fullStr Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection
title_full_unstemmed Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection
title_sort Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection
author Alves, Murilo S.
author_facet Alves, Murilo S.
Soares, Zamira G.
Vidigal, Pedro M. P.
Barros, Everaldo G.
Poddanosqui, Adriana M. P.
Aoyagi, Luciano N.
Abdelnoor, Ricardo V.
Marcelino-Guimarães, Francismar C.
Fietto, Luciano G.
author_role author
author2 Soares, Zamira G.
Vidigal, Pedro M. P.
Barros, Everaldo G.
Poddanosqui, Adriana M. P.
Aoyagi, Luciano N.
Abdelnoor, Ricardo V.
Marcelino-Guimarães, Francismar C.
Fietto, Luciano G.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Murilo S.
Soares, Zamira G.
Vidigal, Pedro M. P.
Barros, Everaldo G.
Poddanosqui, Adriana M. P.
Aoyagi, Luciano N.
Abdelnoor, Ricardo V.
Marcelino-Guimarães, Francismar C.
Fietto, Luciano G.
dc.subject.pt-BR.fl_str_mv Biotic stress
Signal transduction
Plant defense
Asian soybean rust
Transcriptional control
topic Biotic stress
Signal transduction
Plant defense
Asian soybean rust
Transcriptional control
description Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is one of most important diseases in the soybean (Glycine max (L.) Merr.) agribusiness. The identification and characterization of genes related to plant defense responses to fungal infection are essential to develop ASR-resistant plants. In this work, we describe four soybean genes, GmbZIP62, GmbZIP105, GmbZIPE1, and GmbZIPE2, which encode transcription factors containing a basic leucine zipper (bZIP) domain from two divergent classes, and that are responsive to P. pachyrhizi infection. Molecular phylogenetic analyses demonstrated that these genes encode proteins similar to bZIP factors responsive to pathogens. Yeast transactivation assays showed that only GmbZIP62 has strong transactivation activity in yeast. In addition, three of the bZIP transcription factors analyzed were also differentially expressed by plant defense hormones, and all were differentially expressed by fungal attack, indicating that these proteins might participate in response to ASR infection. The results suggested that these bZIP proteins are part of the plant defense response to P. pachyrhizi infection, by regulating the gene expression related to ASR infection responses. These bZIP genes are potential targets to obtain new soybean genotypes resistant to ASR.
publishDate 2015
dc.date.issued.fl_str_mv 2015-05-27
dc.date.accessioned.fl_str_mv 2018-05-04T13:22:18Z
dc.date.available.fl_str_mv 2018-05-04T13:22:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://doi.org/10.1007/s10142-015-0445-0
http://www.locus.ufv.br/handle/123456789/19328
dc.identifier.issn.none.fl_str_mv 14387948
identifier_str_mv 14387948
url https://doi.org/10.1007/s10142-015-0445-0
http://www.locus.ufv.br/handle/123456789/19328
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 15, Issue 6, p. 685–696, November 2015
dc.rights.driver.fl_str_mv Springer-Verlag Berlin Heidelberg
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Springer-Verlag Berlin Heidelberg
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Functional & Integrative Genomics
publisher.none.fl_str_mv Functional & Integrative Genomics
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