Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.anifeedsci.2016.10.009 http://www.locus.ufv.br/handle/123456789/21338 |
Resumo: | It was evaluated the impact of the diet switch on nutritional characteristics and bacterial community composition in cattle fed tropical forage-based diet aiming to establish the minimal length of the adaptation period that could be applied to changeover or crossover design protocols. Twelve rumen-cannulated crossbreed (B. taurus × B. indicus) bovines were used in this experiment (four young bulls, four steers, and four heifers). The experiment lasted 60 days and two diet switches were done at d 21 and d 41. Four diet sequences were evaluated (one animal of each category per sequence): sugarcane-corn silage-sugarcane, corn silage-sugarcane-corn silage, high-low-high concentrate diets, and low-high-low concentrate diets. All diets were adjusted to 110 g crude protein/kg dry matter by using urea. Four groups of variables were evaluated along experimental days: voluntary intake characteristics, fecal excretion and composition, ruminal digesta compostion, and ruminal fermentation profile. The pattern of the groups along time was interpreted through a non-hierarchical clustering procedure and the stabilization of each group of characteristics was achieved within 9–13, 9–14, 6–13, and 4–11 days after diet switch, respectively. The diversity of the liquid-associated rumen bacterial community was also evaluated by using PCR-DGGE in the young bulls from 6 days before to 20 days after the second diet switch. The pattern of microbial diversity was evaluated through a clustering procedure using the unweight pair group method with arithmetic mean. The bacterial community in the liquid phase stabilized within 3–9 days after diet switch. From the results of this experiment, among all obtained times to adapt intake, fecal and digesta composition, and ruminal fermentation and microbial diversity, a 14-days adaptation period is recommended for changeover and crossover experiments with cattle fed tropical forage-based diets. |
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Machado, Marcelo G.Detmann, EdenioMantovani, Hilário C.Valadares Filho, Sebastião C.Bento, Cláudia B.P.Marcondes, Marcos I.Assunção, Amanda S.2018-08-22T20:21:17Z2018-08-22T20:21:17Z2016-1203778401https://doi.org/10.1016/j.anifeedsci.2016.10.009http://www.locus.ufv.br/handle/123456789/21338It was evaluated the impact of the diet switch on nutritional characteristics and bacterial community composition in cattle fed tropical forage-based diet aiming to establish the minimal length of the adaptation period that could be applied to changeover or crossover design protocols. Twelve rumen-cannulated crossbreed (B. taurus × B. indicus) bovines were used in this experiment (four young bulls, four steers, and four heifers). The experiment lasted 60 days and two diet switches were done at d 21 and d 41. Four diet sequences were evaluated (one animal of each category per sequence): sugarcane-corn silage-sugarcane, corn silage-sugarcane-corn silage, high-low-high concentrate diets, and low-high-low concentrate diets. All diets were adjusted to 110 g crude protein/kg dry matter by using urea. Four groups of variables were evaluated along experimental days: voluntary intake characteristics, fecal excretion and composition, ruminal digesta compostion, and ruminal fermentation profile. The pattern of the groups along time was interpreted through a non-hierarchical clustering procedure and the stabilization of each group of characteristics was achieved within 9–13, 9–14, 6–13, and 4–11 days after diet switch, respectively. The diversity of the liquid-associated rumen bacterial community was also evaluated by using PCR-DGGE in the young bulls from 6 days before to 20 days after the second diet switch. The pattern of microbial diversity was evaluated through a clustering procedure using the unweight pair group method with arithmetic mean. The bacterial community in the liquid phase stabilized within 3–9 days after diet switch. From the results of this experiment, among all obtained times to adapt intake, fecal and digesta composition, and ruminal fermentation and microbial diversity, a 14-days adaptation period is recommended for changeover and crossover experiments with cattle fed tropical forage-based diets.engAnimal Feed Science and Technologyv. 222, p. 132- 148, dez. 2016Elsevier B.V.info:eu-repo/semantics/openAccessCarryover designsChangeover designsCarry-over effectDGGEEvaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based dietsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf2644456https://locus.ufv.br//bitstream/123456789/21338/1/artigo.pdf8cbd3e1f20159292919a3b3e56575981MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/21338/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg4547https://locus.ufv.br//bitstream/123456789/21338/3/artigo.pdf.jpg4db3cb7866ad29fba345a1964d5e11d5MD53123456789/213382018-08-22 23:00:54.045oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-08-23T02:00:54LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets |
title |
Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets |
spellingShingle |
Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets Machado, Marcelo G. Carryover designs Changeover designs Carry-over effect DGGE |
title_short |
Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets |
title_full |
Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets |
title_fullStr |
Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets |
title_full_unstemmed |
Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets |
title_sort |
Evaluation of the length of adaptation period for changeover and crossover nutritional experiments with cattle fed tropical forage-based diets |
author |
Machado, Marcelo G. |
author_facet |
Machado, Marcelo G. Detmann, Edenio Mantovani, Hilário C. Valadares Filho, Sebastião C. Bento, Cláudia B.P. Marcondes, Marcos I. Assunção, Amanda S. |
author_role |
author |
author2 |
Detmann, Edenio Mantovani, Hilário C. Valadares Filho, Sebastião C. Bento, Cláudia B.P. Marcondes, Marcos I. Assunção, Amanda S. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Machado, Marcelo G. Detmann, Edenio Mantovani, Hilário C. Valadares Filho, Sebastião C. Bento, Cláudia B.P. Marcondes, Marcos I. Assunção, Amanda S. |
dc.subject.pt-BR.fl_str_mv |
Carryover designs Changeover designs Carry-over effect DGGE |
topic |
Carryover designs Changeover designs Carry-over effect DGGE |
description |
It was evaluated the impact of the diet switch on nutritional characteristics and bacterial community composition in cattle fed tropical forage-based diet aiming to establish the minimal length of the adaptation period that could be applied to changeover or crossover design protocols. Twelve rumen-cannulated crossbreed (B. taurus × B. indicus) bovines were used in this experiment (four young bulls, four steers, and four heifers). The experiment lasted 60 days and two diet switches were done at d 21 and d 41. Four diet sequences were evaluated (one animal of each category per sequence): sugarcane-corn silage-sugarcane, corn silage-sugarcane-corn silage, high-low-high concentrate diets, and low-high-low concentrate diets. All diets were adjusted to 110 g crude protein/kg dry matter by using urea. Four groups of variables were evaluated along experimental days: voluntary intake characteristics, fecal excretion and composition, ruminal digesta compostion, and ruminal fermentation profile. The pattern of the groups along time was interpreted through a non-hierarchical clustering procedure and the stabilization of each group of characteristics was achieved within 9–13, 9–14, 6–13, and 4–11 days after diet switch, respectively. The diversity of the liquid-associated rumen bacterial community was also evaluated by using PCR-DGGE in the young bulls from 6 days before to 20 days after the second diet switch. The pattern of microbial diversity was evaluated through a clustering procedure using the unweight pair group method with arithmetic mean. The bacterial community in the liquid phase stabilized within 3–9 days after diet switch. From the results of this experiment, among all obtained times to adapt intake, fecal and digesta composition, and ruminal fermentation and microbial diversity, a 14-days adaptation period is recommended for changeover and crossover experiments with cattle fed tropical forage-based diets. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12 |
dc.date.accessioned.fl_str_mv |
2018-08-22T20:21:17Z |
dc.date.available.fl_str_mv |
2018-08-22T20:21:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.anifeedsci.2016.10.009 http://www.locus.ufv.br/handle/123456789/21338 |
dc.identifier.issn.none.fl_str_mv |
03778401 |
identifier_str_mv |
03778401 |
url |
https://doi.org/10.1016/j.anifeedsci.2016.10.009 http://www.locus.ufv.br/handle/123456789/21338 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 222, p. 132- 148, dez. 2016 |
dc.rights.driver.fl_str_mv |
Elsevier B.V. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Elsevier B.V. |
eu_rights_str_mv |
openAccess |
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dc.publisher.none.fl_str_mv |
Animal Feed Science and Technology |
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Animal Feed Science and Technology |
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LOCUS Repositório Institucional da UFV |
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