Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/2378 |
Resumo: | The age polyethism of Apis mellifera workers is related to morphological and physiological changes in many systems, including the digestive system, and to diet change of the bees, younger workers consuming mainly pollen and older bees consuming honey. The aquaporins are membrane proteins that have been identified in different organs of the digestive tract of insects and are responsible for transmembrane transport of water. This study evaluated whether there are differences in the expression of aqp-4-like, a gene for aquaporin in A. mellifera bees between nurses and foragers workers. The Malpighian tubules, crop, midgut, ileum and rectum of workers with different tasks were used to perform qPCR. The results showed that in nurse bees aqp-4-like mRNA was greater expressed in Malpighian tubules and ileum, while in the forager the highest expression occurred in the crop and rectum. The midgut was the only organ that showed no difference in expression between nurses and foragers, but it was the organ with the highest expression of aqp-4-like compared to the Malpighian tubules. These data show that AQP-4-like is differentially expressed in various organs of the digestive tract of nurses and foragers workers due to its different diets and physiology. The differential expression of the gene is related to osmoregulation in the Malpighian tubules, ileum and rectum. Also, the dehydration of the food content in the crop and midgut by this aquaporin avoids the digestive enzymes dilution, ensuring the completion of digestion. |
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Souza, Débora Linhares Linohttp://lattes.cnpq.br/2563185742178779Serrão, José Eduardohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785636U6Silva, Cynthia Canedo dahttp://lattes.cnpq.br/7077220592875119Campos, Lúcio Antonio de Oliveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783908P92015-03-26T13:04:21Z2015-01-202015-03-26T13:04:21Z2014-05-15SOUZA, Débora Linhares Lino. Expresson of the gene aqp-4-like in the digestive system of Apis mellifera (Hymenoptera: Apidae) workers. 2014. 43 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/2378The age polyethism of Apis mellifera workers is related to morphological and physiological changes in many systems, including the digestive system, and to diet change of the bees, younger workers consuming mainly pollen and older bees consuming honey. The aquaporins are membrane proteins that have been identified in different organs of the digestive tract of insects and are responsible for transmembrane transport of water. This study evaluated whether there are differences in the expression of aqp-4-like, a gene for aquaporin in A. mellifera bees between nurses and foragers workers. The Malpighian tubules, crop, midgut, ileum and rectum of workers with different tasks were used to perform qPCR. The results showed that in nurse bees aqp-4-like mRNA was greater expressed in Malpighian tubules and ileum, while in the forager the highest expression occurred in the crop and rectum. The midgut was the only organ that showed no difference in expression between nurses and foragers, but it was the organ with the highest expression of aqp-4-like compared to the Malpighian tubules. These data show that AQP-4-like is differentially expressed in various organs of the digestive tract of nurses and foragers workers due to its different diets and physiology. The differential expression of the gene is related to osmoregulation in the Malpighian tubules, ileum and rectum. Also, the dehydration of the food content in the crop and midgut by this aquaporin avoids the digestive enzymes dilution, ensuring the completion of digestion.O polietismo etário das operárias de Apis mellifera está relacionado a alterações morfofisiológicas de diversos sistemas, incluindo o sistema digestório, e à mudança da dieta das abelhas, sendo que operárias mais jovens consomem principalmente pólen e as mais velhas mel. As aquaporinas são proteínas de membrana identificadas em diferentes órgãos do trato digestório de insetos e são responsáveis pelo transporte transmembrana de água. Este trabalho avaliou se há diferença na expressão de aqp-4-like, um gene para aquaporina de A. mellifera, entre abelhas nutridoras e campeiras. Os túbulos de Malpighi, papo, intestino médio, intestino fino e reto de operárias exercendo diferentes funções foram utilizados na realização de qPCR. Os resultados demonstraram que em abelhas nutridoras houve maior expressão de mRNA para aqp-4-like nos túbulos de Malpighi e no intestino fino, enquanto que nas campeiras ocorreu maior expressão no papo e reto. O intestino médio foi o único órgão que não apresentou diferença de expressão entre nutridoras e campeiras, mas foi o órgão com maior expressão de aqp-4-like quando comparado aos túbulos de Malpighi. Estes dados mostram que a AQP-4-like é diferencialmente expressa em vários órgãos do trato digestório de operárias nutridoras e campeiras devido às diferentes dietas e fisiologia. A expressão diferencial do gene está relacionada à osmorregulação nos túbulos de Malpighi, intestino fino e reto. A desidratação do alimento no papo e intestino médio por esta aquaporina evita a diluição de enzimas digestivas, garantindo a realização da digestão.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Biologia Celular e EstruturalUFVBRAnálises quantitativas e moleculares do Genoma; Biologia das células e dos tecidosAbelhaAquaporinaProteínasFisiologiaBeeAquaporinProteinsPhysiologyCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALExpressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae)Expresson of the gene aqp-4-like in the digestive system of Apis mellifera (Hymenoptera: Apidae) workersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf318912https://locus.ufv.br//bitstream/123456789/2378/1/texto%20completo.pdfd73ab52d07968e7249563f0272104f15MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain63599https://locus.ufv.br//bitstream/123456789/2378/2/texto%20completo.pdf.txt618be02914bf0d801018fa6b2cb56e5bMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3586https://locus.ufv.br//bitstream/123456789/2378/3/texto%20completo.pdf.jpgf493faa1df2789cc99d93257fd9d8f78MD53123456789/23782016-04-08 23:05:16.1oai:locus.ufv.br:123456789/2378Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-09T02:05:16LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae) |
dc.title.alternative.eng.fl_str_mv |
Expresson of the gene aqp-4-like in the digestive system of Apis mellifera (Hymenoptera: Apidae) workers |
title |
Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae) |
spellingShingle |
Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae) Souza, Débora Linhares Lino Abelha Aquaporina Proteínas Fisiologia Bee Aquaporin Proteins Physiology CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae) |
title_full |
Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae) |
title_fullStr |
Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae) |
title_full_unstemmed |
Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae) |
title_sort |
Expressão do gene aqp-4-like no trato digestório de operárias de Apis mellifera (Hymenoptera: Apidae) |
author |
Souza, Débora Linhares Lino |
author_facet |
Souza, Débora Linhares Lino |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/2563185742178779 |
dc.contributor.author.fl_str_mv |
Souza, Débora Linhares Lino |
dc.contributor.advisor1.fl_str_mv |
Serrão, José Eduardo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785636U6 |
dc.contributor.referee1.fl_str_mv |
Silva, Cynthia Canedo da |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7077220592875119 |
dc.contributor.referee2.fl_str_mv |
Campos, Lúcio Antonio de Oliveira |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783908P9 |
contributor_str_mv |
Serrão, José Eduardo Silva, Cynthia Canedo da Campos, Lúcio Antonio de Oliveira |
dc.subject.por.fl_str_mv |
Abelha Aquaporina Proteínas Fisiologia |
topic |
Abelha Aquaporina Proteínas Fisiologia Bee Aquaporin Proteins Physiology CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Bee Aquaporin Proteins Physiology |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
The age polyethism of Apis mellifera workers is related to morphological and physiological changes in many systems, including the digestive system, and to diet change of the bees, younger workers consuming mainly pollen and older bees consuming honey. The aquaporins are membrane proteins that have been identified in different organs of the digestive tract of insects and are responsible for transmembrane transport of water. This study evaluated whether there are differences in the expression of aqp-4-like, a gene for aquaporin in A. mellifera bees between nurses and foragers workers. The Malpighian tubules, crop, midgut, ileum and rectum of workers with different tasks were used to perform qPCR. The results showed that in nurse bees aqp-4-like mRNA was greater expressed in Malpighian tubules and ileum, while in the forager the highest expression occurred in the crop and rectum. The midgut was the only organ that showed no difference in expression between nurses and foragers, but it was the organ with the highest expression of aqp-4-like compared to the Malpighian tubules. These data show that AQP-4-like is differentially expressed in various organs of the digestive tract of nurses and foragers workers due to its different diets and physiology. The differential expression of the gene is related to osmoregulation in the Malpighian tubules, ileum and rectum. Also, the dehydration of the food content in the crop and midgut by this aquaporin avoids the digestive enzymes dilution, ensuring the completion of digestion. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-05-15 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:04:21Z |
dc.date.available.fl_str_mv |
2015-01-20 2015-03-26T13:04:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUZA, Débora Linhares Lino. Expresson of the gene aqp-4-like in the digestive system of Apis mellifera (Hymenoptera: Apidae) workers. 2014. 43 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2014. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/2378 |
identifier_str_mv |
SOUZA, Débora Linhares Lino. Expresson of the gene aqp-4-like in the digestive system of Apis mellifera (Hymenoptera: Apidae) workers. 2014. 43 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2014. |
url |
http://locus.ufv.br/handle/123456789/2378 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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Universidade Federal de Viçosa |
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Mestrado em Biologia Celular e Estrutural |
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UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos |
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Universidade Federal de Viçosa |
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