Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/2356 |
Resumo: | Among the many clinical and laboratory differences observed in leishmaniasis, a change that stands out is the hypergammaglobulinemia that occurs especially during visceral leishmaniasis caused by L. chagasi. Faced with the characteristic of this specie in activate B cell clones nonspecifically, leading to indiscriminate production of antibodies, the present study was designed to evaluate the effect of administration of crude extract of L. chagasi (EPLc) or L. braziliensis (EPLb), a specie which causes mucocutaneous leishmaniasis, associated or not with incomplete Freund's adjuvant (AIF) or alum (ALU), in the production of specific IgG antibodies against ovalbumin (OVA) in C57BL/6 mice. Furthermore the levels of mRNA to IFN-γ and IL4 in spleen cells from the same animals, aiming to evaluate the profile of immune response induced by the extracts. The results showed that: 1. Both EPLb as EPLc, in the absence of OVA, induce higher production of anti-OVA IgG than the control group (PBS). Interestingly, both extracts decrease the production of anti-OVA IgG in the presence of adjuvants and OVA, and only EPLb is able to decrease the production of IgG in the presence of OVA alone; 2. Only EPLb is able to reduce the levels of IgG1 induced by OVA; 3. EPLb, in the presence of OVA, seems to decrease the production of mRNA to IL4 more effectively than EPLc; 4. Considering the production of IFN-γ mRNA, both extracts show the same profile, and are not able to induce a marked production of this cytokine. These immunosuppressive properties of both extracts, particularly EPLb, may be target in future studies, in cases where it is desired to reduce immune system's reactions for mitigate symptoms, for example in hypersensitivity disorders. |
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Valente, Samara Freirehttp://lattes.cnpq.br/8648742344708227Paula, Sérgio Oliveira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P4Silva, Eduardo de Almeida Marques dahttp://lattes.cnpq.br/9196320705613169Cardoso, Silvia Almeidahttp://lattes.cnpq.br/6041368188542057Eller, Monique Renonhttp://lattes.cnpq.br/48217855239067892015-03-26T13:04:17Z2013-06-242015-03-26T13:04:17Z2013-02-28VALENTE, Samara Freire. Profile of immune response induced for particulate extracts from Leishmania braziliensis or Leishmania chagasi in C57BL/6 mouse. 2013. 45 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2013.http://locus.ufv.br/handle/123456789/2356Among the many clinical and laboratory differences observed in leishmaniasis, a change that stands out is the hypergammaglobulinemia that occurs especially during visceral leishmaniasis caused by L. chagasi. Faced with the characteristic of this specie in activate B cell clones nonspecifically, leading to indiscriminate production of antibodies, the present study was designed to evaluate the effect of administration of crude extract of L. chagasi (EPLc) or L. braziliensis (EPLb), a specie which causes mucocutaneous leishmaniasis, associated or not with incomplete Freund's adjuvant (AIF) or alum (ALU), in the production of specific IgG antibodies against ovalbumin (OVA) in C57BL/6 mice. Furthermore the levels of mRNA to IFN-γ and IL4 in spleen cells from the same animals, aiming to evaluate the profile of immune response induced by the extracts. The results showed that: 1. Both EPLb as EPLc, in the absence of OVA, induce higher production of anti-OVA IgG than the control group (PBS). Interestingly, both extracts decrease the production of anti-OVA IgG in the presence of adjuvants and OVA, and only EPLb is able to decrease the production of IgG in the presence of OVA alone; 2. Only EPLb is able to reduce the levels of IgG1 induced by OVA; 3. EPLb, in the presence of OVA, seems to decrease the production of mRNA to IL4 more effectively than EPLc; 4. Considering the production of IFN-γ mRNA, both extracts show the same profile, and are not able to induce a marked production of this cytokine. These immunosuppressive properties of both extracts, particularly EPLb, may be target in future studies, in cases where it is desired to reduce immune system's reactions for mitigate symptoms, for example in hypersensitivity disorders.Dentre as diversas diferenças clínicas e laboratoriais nas leishmanioses, uma alteração que se destaca é a hipergamaglobulinemia, que ocorre especialmente nos casos de leishmaniose visceral, causada por L. chagasi. Frente à característica desta espécie em ativar inespecificamente clones de linfócitos B, levando à produção indiscriminada de anticorpos, no presente trabalho avaliou-se o efeito da administração de extrato particulado de L. chagasi (EPLc) ou de extrato particulado de L. braziliensis (EPLb), espécie causadora de leishmaniose mucocutânea, associados ou não a adjuvante incompleto de Freund (AIF) ou a alúmen (ALU), na produção de anticorpos IgG específicos contra ovalbumina (OVA) em soro de camundongos C57BL/6. Além disso, os níveis de mRNA para IFN-γ e IL-4 em esplenócitos dos mesmos animais, estimulados in vitro com OVA, com o objetivo de avaliar o perfil de resposta imune induzida pelos extratos. Os resultados obtidos mostraram que: 1. Tanto EPLb como EPLc, na ausência de OVA, induzem produção de IgG anti-OVA superior ao grupo controle (PBS). Porém, os dois extratos diminuíram a produção de anticorpos IgG anti-OVA na presença de outros adjuvantes e OVA, EPLb diminui a produção de IgG na presença de OVA; 2. EPLb diminui os níveis de IgG1 induzida por OVA, o que não ocorre na presença de EPLc; 3. EPLb, na presença de OVA, parece diminuir a produção de mRNA de IL-4 mais acentuadamente que EPLc; 4. Quanto à produção de IFN-γ, os dois extratos tiveram perfis semelhantes entre si, e não foram capazes de induzir uma produção acentuada de mRNA para esta citocina. Essas propriedades imunossupressoras de ambos os extratos, em especial do EPLb, podem ser alvos de estudos futuros em casos em que se deseja uma atenuação dos efeitos causados pela resposta imune, como, por exemplo, em casos de hipersensibilidade.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Biologia Celular e EstruturalUFVBRAnálises quantitativas e moleculares do Genoma; Biologia das células e dos tecidosLeishmaniaResposta imuneAnticorpoLeishmaniaImmune responseAntibodyCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALPerfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6Profile of immune response induced for particulate extracts from Leishmania braziliensis or Leishmania chagasi in C57BL/6 mouseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1354923https://locus.ufv.br//bitstream/123456789/2356/1/texto%20completo.pdf83d3ed0876eda52ed9f49475bc0043dbMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain76834https://locus.ufv.br//bitstream/123456789/2356/2/texto%20completo.pdf.txtf7efe9b0b1231b07a29ff83f643e1371MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3478https://locus.ufv.br//bitstream/123456789/2356/3/texto%20completo.pdf.jpg56426547ab772f383848ee614040c9fcMD53123456789/23562016-04-08 23:06:15.806oai:locus.ufv.br:123456789/2356Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-09T02:06:15LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6 |
dc.title.alternative.eng.fl_str_mv |
Profile of immune response induced for particulate extracts from Leishmania braziliensis or Leishmania chagasi in C57BL/6 mouse |
title |
Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6 |
spellingShingle |
Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6 Valente, Samara Freire Leishmania Resposta imune Anticorpo Leishmania Immune response Antibody CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6 |
title_full |
Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6 |
title_fullStr |
Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6 |
title_full_unstemmed |
Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6 |
title_sort |
Perfil de resposta imune induzida por extrato particulado de Leishmania braziliensis ou de Leishmania chagasi em camundongo C57BL/6 |
author |
Valente, Samara Freire |
author_facet |
Valente, Samara Freire |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/8648742344708227 |
dc.contributor.author.fl_str_mv |
Valente, Samara Freire |
dc.contributor.advisor-co1.fl_str_mv |
Paula, Sérgio Oliveira de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P4 |
dc.contributor.advisor1.fl_str_mv |
Silva, Eduardo de Almeida Marques da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9196320705613169 |
dc.contributor.referee1.fl_str_mv |
Cardoso, Silvia Almeida |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6041368188542057 |
dc.contributor.referee2.fl_str_mv |
Eller, Monique Renon |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4821785523906789 |
contributor_str_mv |
Paula, Sérgio Oliveira de Silva, Eduardo de Almeida Marques da Cardoso, Silvia Almeida Eller, Monique Renon |
dc.subject.por.fl_str_mv |
Leishmania Resposta imune Anticorpo |
topic |
Leishmania Resposta imune Anticorpo Leishmania Immune response Antibody CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Leishmania Immune response Antibody |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Among the many clinical and laboratory differences observed in leishmaniasis, a change that stands out is the hypergammaglobulinemia that occurs especially during visceral leishmaniasis caused by L. chagasi. Faced with the characteristic of this specie in activate B cell clones nonspecifically, leading to indiscriminate production of antibodies, the present study was designed to evaluate the effect of administration of crude extract of L. chagasi (EPLc) or L. braziliensis (EPLb), a specie which causes mucocutaneous leishmaniasis, associated or not with incomplete Freund's adjuvant (AIF) or alum (ALU), in the production of specific IgG antibodies against ovalbumin (OVA) in C57BL/6 mice. Furthermore the levels of mRNA to IFN-γ and IL4 in spleen cells from the same animals, aiming to evaluate the profile of immune response induced by the extracts. The results showed that: 1. Both EPLb as EPLc, in the absence of OVA, induce higher production of anti-OVA IgG than the control group (PBS). Interestingly, both extracts decrease the production of anti-OVA IgG in the presence of adjuvants and OVA, and only EPLb is able to decrease the production of IgG in the presence of OVA alone; 2. Only EPLb is able to reduce the levels of IgG1 induced by OVA; 3. EPLb, in the presence of OVA, seems to decrease the production of mRNA to IL4 more effectively than EPLc; 4. Considering the production of IFN-γ mRNA, both extracts show the same profile, and are not able to induce a marked production of this cytokine. These immunosuppressive properties of both extracts, particularly EPLb, may be target in future studies, in cases where it is desired to reduce immune system's reactions for mitigate symptoms, for example in hypersensitivity disorders. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-06-24 2015-03-26T13:04:17Z |
dc.date.issued.fl_str_mv |
2013-02-28 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:04:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VALENTE, Samara Freire. Profile of immune response induced for particulate extracts from Leishmania braziliensis or Leishmania chagasi in C57BL/6 mouse. 2013. 45 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2013. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/2356 |
identifier_str_mv |
VALENTE, Samara Freire. Profile of immune response induced for particulate extracts from Leishmania braziliensis or Leishmania chagasi in C57BL/6 mouse. 2013. 45 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2013. |
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http://locus.ufv.br/handle/123456789/2356 |
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por |
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Universidade Federal de Viçosa |
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Mestrado em Biologia Celular e Estrutural |
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UFV |
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BR |
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Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos |
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Universidade Federal de Viçosa |
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