The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1093/jxb/erg301 http://www.locus.ufv.br/handle/123456789/14868 |
Resumo: | The sucrose binding protein (SBP) from soybean has been implicated as an important component of the sucrose uptake system. Two SBP genomic clones, gsS641.1 and gsS641.2, which correspond to allelic forms of the GmSBP2/S64 gene, have been isolated and characterized. As a member of the seed storage protein superfamily, it has been shown that the SBP gene structure is similar to vicilin genes with intron/exon boundaries at conserved positions. Fluores cence in situ hybridization (FISH) suggested that the soybean SBP gene family is represented by at least two non‐allelic genes corresponding to the previously isolated GmSBP1 and GmSBP2/S64 cDNAs. These two cDNAs share extensive sequence similarity but are located at different loci in the soybean genome. To investigate transcriptional activation of the GmSBP2 gene, 2 kb 5′‐flanking sequences of gsS641.1 and gsS641.2 were fused to the β‐glucuronidase (GUS) reporter gene and to the green fluorescent protein (GFP) reporter gene and inde pendently introduced into Nicotiana tabacum by Agrobacterium tumefaciens‐mediated transformation. The SBP2 promoter directed expression of both GUS and GFP reporter genes with high specificity to the phloem of leaves, stems and roots. Thus, the overall pattern of SBP–GUS or SBP–GFP expression is consistent with the involvement of SBP in sucrose translocation‐dependent physiological processes. |
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The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoterFISHGene familyGlycine maxPhloem‐specific expressionPromoter activitySucrose binding proteinThe sucrose binding protein (SBP) from soybean has been implicated as an important component of the sucrose uptake system. Two SBP genomic clones, gsS641.1 and gsS641.2, which correspond to allelic forms of the GmSBP2/S64 gene, have been isolated and characterized. As a member of the seed storage protein superfamily, it has been shown that the SBP gene structure is similar to vicilin genes with intron/exon boundaries at conserved positions. Fluores cence in situ hybridization (FISH) suggested that the soybean SBP gene family is represented by at least two non‐allelic genes corresponding to the previously isolated GmSBP1 and GmSBP2/S64 cDNAs. These two cDNAs share extensive sequence similarity but are located at different loci in the soybean genome. To investigate transcriptional activation of the GmSBP2 gene, 2 kb 5′‐flanking sequences of gsS641.1 and gsS641.2 were fused to the β‐glucuronidase (GUS) reporter gene and to the green fluorescent protein (GFP) reporter gene and inde pendently introduced into Nicotiana tabacum by Agrobacterium tumefaciens‐mediated transformation. The SBP2 promoter directed expression of both GUS and GFP reporter genes with high specificity to the phloem of leaves, stems and roots. Thus, the overall pattern of SBP–GUS or SBP–GFP expression is consistent with the involvement of SBP in sucrose translocation‐dependent physiological processes.Journal of Experimental Botany2017-12-13T12:38:49Z2017-12-13T12:38:49Z2003-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1460-2431https://doi.org/10.1093/jxb/erg301http://www.locus.ufv.br/handle/123456789/14868engVol. 54, N. 393, p. 2643-2653, December 2003Contim, Luis Antônio S.Waclawovsky, Alessandro J.Delú‐Filho, NelsonPirovani, Carlos P.Clarindo, Wellington R.Loureiro, Marcelo E.Carvalho, Carlos R.Fontes, Elizabeth P. B.info:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:59:09Zoai:locus.ufv.br:123456789/14868Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:59:09LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter |
title |
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter |
spellingShingle |
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter Contim, Luis Antônio S. FISH Gene family Glycine max Phloem‐specific expression Promoter activity Sucrose binding protein |
title_short |
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter |
title_full |
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter |
title_fullStr |
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter |
title_full_unstemmed |
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter |
title_sort |
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue‐specific expression of the SBP2 promoter |
author |
Contim, Luis Antônio S. |
author_facet |
Contim, Luis Antônio S. Waclawovsky, Alessandro J. Delú‐Filho, Nelson Pirovani, Carlos P. Clarindo, Wellington R. Loureiro, Marcelo E. Carvalho, Carlos R. Fontes, Elizabeth P. B. |
author_role |
author |
author2 |
Waclawovsky, Alessandro J. Delú‐Filho, Nelson Pirovani, Carlos P. Clarindo, Wellington R. Loureiro, Marcelo E. Carvalho, Carlos R. Fontes, Elizabeth P. B. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Contim, Luis Antônio S. Waclawovsky, Alessandro J. Delú‐Filho, Nelson Pirovani, Carlos P. Clarindo, Wellington R. Loureiro, Marcelo E. Carvalho, Carlos R. Fontes, Elizabeth P. B. |
dc.subject.por.fl_str_mv |
FISH Gene family Glycine max Phloem‐specific expression Promoter activity Sucrose binding protein |
topic |
FISH Gene family Glycine max Phloem‐specific expression Promoter activity Sucrose binding protein |
description |
The sucrose binding protein (SBP) from soybean has been implicated as an important component of the sucrose uptake system. Two SBP genomic clones, gsS641.1 and gsS641.2, which correspond to allelic forms of the GmSBP2/S64 gene, have been isolated and characterized. As a member of the seed storage protein superfamily, it has been shown that the SBP gene structure is similar to vicilin genes with intron/exon boundaries at conserved positions. Fluores cence in situ hybridization (FISH) suggested that the soybean SBP gene family is represented by at least two non‐allelic genes corresponding to the previously isolated GmSBP1 and GmSBP2/S64 cDNAs. These two cDNAs share extensive sequence similarity but are located at different loci in the soybean genome. To investigate transcriptional activation of the GmSBP2 gene, 2 kb 5′‐flanking sequences of gsS641.1 and gsS641.2 were fused to the β‐glucuronidase (GUS) reporter gene and to the green fluorescent protein (GFP) reporter gene and inde pendently introduced into Nicotiana tabacum by Agrobacterium tumefaciens‐mediated transformation. The SBP2 promoter directed expression of both GUS and GFP reporter genes with high specificity to the phloem of leaves, stems and roots. Thus, the overall pattern of SBP–GUS or SBP–GFP expression is consistent with the involvement of SBP in sucrose translocation‐dependent physiological processes. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-12-01 2017-12-13T12:38:49Z 2017-12-13T12:38:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
1460-2431 https://doi.org/10.1093/jxb/erg301 http://www.locus.ufv.br/handle/123456789/14868 |
identifier_str_mv |
1460-2431 |
url |
https://doi.org/10.1093/jxb/erg301 http://www.locus.ufv.br/handle/123456789/14868 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vol. 54, N. 393, p. 2643-2653, December 2003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Journal of Experimental Botany |
publisher.none.fl_str_mv |
Journal of Experimental Botany |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1822610592955891712 |