Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/4395 |
Resumo: | Nowadays, Brazil is the largest producer of papaya and the third largest exporter of fruit in the world. Papaya can be affected by various diseases that prevent them from being marketed and, consequently, the exportation of the fruit. Among the fungal diseases, smallpox, or blight, caused by Asperisporium caricae, is one of the diseases that affect the orchards in all regions. This disease interferes directly in the profitability and viability of the production. Beyond its incidence on leaves, it can also cause lesions on the fruit surface, making them unsuitable for marketing. It s almost impossible to find publications related to the biology of the fungus and to the phylogenetic position of the species. A. caricae is considered by many authors as a biotrophic pathogen, based on the difficulty of cultivation and sporulation in vitro. Some aspects of the taxonomic genus Asperisporium are controversial, such as proving Mycosphaerella as a teleomorph organism, the phylogenetic relationships with other genera of the complex cercosporoidal and the separation of the genus Asperisporium from Passalora. Other authors relate M. caricae to the anamorph Phoma caricae-papaya (= Ascochyta caricapapaya), causal agent of stalk rot. However, through the manual identification the genera Phoma and Ascochyta are related to the teleomorph Didymella. The differences between the genders Didymella and Mycosphaerella is the presence of pseudo paraphyses in the last one. This feature is difficult to visualize in histological sections under a light microscope, thus being liable to misunderstand at the time of identification. The aims of the study were the isolation of A. caricae in pure culture, examining the growth and sporulation of the isolates in seven different media and make a morphological characterization of isolates. Beyond these, other aim was to verify the relationship between Asperisporium caricae and Mycosphaerella caricae by comparing nucleotides of three DNA regions (ITS rDNA, LSU and mtSSU) and the phylogenetic relationships with other genera of the complex cercosporoidal, by comparing the sequences obtained to sequences published in databases. It was possible to cultivate A. caricae in all tested media. The culture medium potato dextrose agar, supplemented or not with amino acids, were those who presented the best source to the fungus. Moreover, the species produced spores in vitro in all tested media, except in the medium consisting of papaya leaves. Through the molecular data and clusters obtained, it can be concluded that A. caricae belongs to the family Mycosphaerellaceae grouping it with species of true Passalora and Mycosphaerella. M. caricae was grouped with species of the genus Phoma, Ascochyta and Didymella, representatives of the order Pleosporales. Thus, it is possible that M. caricae is a species of Didymella and teleomorph of Phoma carica-papaya. |
id |
UFV_5f606a31213e8bae7a1b9cbff80806c1 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/4395 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Silva, Larissa Gomes dahttp://lattes.cnpq.br/0110287926259408Mizubuti, Eduardo Seiti Gomidehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8Barreto, Robert Weingarthttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783300H6Pereira, Olinto Liparinihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4Evans, Harold Charleshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4501250T6Paula Júnior, Trazilbo José dehttp://lattes.cnpq.br/7899276097018876Nascimento, Robson José dohttp://lattes.cnpq.br/55744226951995652015-03-26T13:37:45Z2012-03-162015-03-26T13:37:45Z2010-02-23SILVA, Larissa Gomes da. Isolation and growth of Asperisporium caricae and their phylogenetic relationship with Mycosphaerellaceae. 2010. 69 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010.http://locus.ufv.br/handle/123456789/4395Nowadays, Brazil is the largest producer of papaya and the third largest exporter of fruit in the world. Papaya can be affected by various diseases that prevent them from being marketed and, consequently, the exportation of the fruit. Among the fungal diseases, smallpox, or blight, caused by Asperisporium caricae, is one of the diseases that affect the orchards in all regions. This disease interferes directly in the profitability and viability of the production. Beyond its incidence on leaves, it can also cause lesions on the fruit surface, making them unsuitable for marketing. It s almost impossible to find publications related to the biology of the fungus and to the phylogenetic position of the species. A. caricae is considered by many authors as a biotrophic pathogen, based on the difficulty of cultivation and sporulation in vitro. Some aspects of the taxonomic genus Asperisporium are controversial, such as proving Mycosphaerella as a teleomorph organism, the phylogenetic relationships with other genera of the complex cercosporoidal and the separation of the genus Asperisporium from Passalora. Other authors relate M. caricae to the anamorph Phoma caricae-papaya (= Ascochyta caricapapaya), causal agent of stalk rot. However, through the manual identification the genera Phoma and Ascochyta are related to the teleomorph Didymella. The differences between the genders Didymella and Mycosphaerella is the presence of pseudo paraphyses in the last one. This feature is difficult to visualize in histological sections under a light microscope, thus being liable to misunderstand at the time of identification. The aims of the study were the isolation of A. caricae in pure culture, examining the growth and sporulation of the isolates in seven different media and make a morphological characterization of isolates. Beyond these, other aim was to verify the relationship between Asperisporium caricae and Mycosphaerella caricae by comparing nucleotides of three DNA regions (ITS rDNA, LSU and mtSSU) and the phylogenetic relationships with other genera of the complex cercosporoidal, by comparing the sequences obtained to sequences published in databases. It was possible to cultivate A. caricae in all tested media. The culture medium potato dextrose agar, supplemented or not with amino acids, were those who presented the best source to the fungus. Moreover, the species produced spores in vitro in all tested media, except in the medium consisting of papaya leaves. Through the molecular data and clusters obtained, it can be concluded that A. caricae belongs to the family Mycosphaerellaceae grouping it with species of true Passalora and Mycosphaerella. M. caricae was grouped with species of the genus Phoma, Ascochyta and Didymella, representatives of the order Pleosporales. Thus, it is possible that M. caricae is a species of Didymella and teleomorph of Phoma carica-papaya.O Brasil é o maior produtor mundial de mamão e o terceiro maior exportador da fruta. O mamoeiro pode ser afetado por diversas doenças que inviabilizam a sua comercialização e, consequentemente, a exportações de frutos. Dentre as doenças fúngicas, a varíola, ou pinta preta, causada por Asperisporium caricae, é uma das doenças que afetam os pomares em todas as regiões produtoras. Interfere diretamente na rentabilidade e viabilidade da produção, pois, além de incidir em folhas, pode também causar lesões na superfície dos frutos, tornando-os inadequados para a comercialização. Trabalhos referentes à biologia do fungo são quase inexistentes, bem como sobre o posicionamento filogenético da espécie. A. caricae é considerado por muitos autores como biotrófico, baseado na dificuldade de cultivo e esporulação in vitro. Alguns aspectos taxonômicos do gênero Asperisporium são controversos, como a comprovação de Mycosphaerella como teleomorfo, o relacionamento filogenético com os demais gêneros do complexo cercosporóide e a separação de Asperisporium do gênero Passalora. Outros autores relacionam M. caricae ao anamorfo Phoma caricae-papaya (=Ascochyta caricae-papayae), agente etiológico da podridão peduncular. Contudo, por meio dos manuais de identificação os gêneros Phoma e Ascochyta estão relacionados ao teleomorfo Didymella. A distinção entre os gêneros Didymella e Mycosphaerella está na presença de pseudoparáfises neste último, sendo esta característica dificilmente visualizada em cortes histológicos em microscópio de luz, deste modo, sendo passível o equívoco no momento da identificação. Os objetivos do trabalho foram: realizar o isolamento de A. caricae em cultura pura, analisar o crescimento e a capacidade de esporulação dos isolados em sete diferentes meios de cultura e proceder a caracterização morfológica dos isolados; verificar a relação entre Asperisporium caricae e Mycosphaerella caricae por meio da comparação de nucleotídeos de três regiões do DNA (ITS do rDNA, LSU e mtSSU), assim como o relacionamento filogenético com os demais gêneros pertencentes ao complexo cercosporóide, por meio da comparação das sequências obtidas com as sequências publicadas em bancos de dados. Foi possível o cultivo de A. caricae em todos os meios de cultura testados, sendo que os meios de cultura batata-dextrose-ágar, suplementado ou não com aminoácidos, foram os que proporcionaram melhor desenvolvimento ao fungo. Além disso, a espécie produziu esporos in vitro em todos os meios de cultura testados, exceto no meio composto por folhas de mamão. Por meio dos dados moleculares e dos agrupamentos obtidos, pode-se concluir que A. caricae pertence à família Mycosphaerellaceae, agrupando com espécies de Passalora verdadeiras e de Mycosphaerella. M. caricae agrupou-se com espécies pertencentes ao gênero Phoma, Didymella e Ascochyta, representantes da ordem Pleosporales. Com isso, é possível que M. caricae seja uma espécie de Didymella, e teleomorfo de Phoma caricae-papayae.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleMycosphaerellaceaeFungos fitopatogênicosFilogeniaMicologiaMamãoMycosphaerellaceaePhytopathogenic fungiPhylogenyMycologyPapayaCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAIsolamento e crescimento de Asperisporium caricae e sua relação filogenética com MycosphaerellaceaeIsolation and growth of Asperisporium caricae and their phylogenetic relationship with Mycosphaerellaceaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf4648060https://locus.ufv.br//bitstream/123456789/4395/1/texto%20completo.pdf02f10a0cc1d0bf768aa16e7168f46ef9MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain97456https://locus.ufv.br//bitstream/123456789/4395/2/texto%20completo.pdf.txt20144bf0fc576ce9e89d0e2580567743MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3577https://locus.ufv.br//bitstream/123456789/4395/3/texto%20completo.pdf.jpg2984204d459f4ceb642d1aa3cb990106MD53123456789/43952016-04-10 23:08:40.052oai:locus.ufv.br:123456789/4395Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:08:40LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae |
dc.title.alternative.eng.fl_str_mv |
Isolation and growth of Asperisporium caricae and their phylogenetic relationship with Mycosphaerellaceae |
title |
Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae |
spellingShingle |
Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae Silva, Larissa Gomes da Mycosphaerellaceae Fungos fitopatogênicos Filogenia Micologia Mamão Mycosphaerellaceae Phytopathogenic fungi Phylogeny Mycology Papaya CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
title_short |
Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae |
title_full |
Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae |
title_fullStr |
Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae |
title_full_unstemmed |
Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae |
title_sort |
Isolamento e crescimento de Asperisporium caricae e sua relação filogenética com Mycosphaerellaceae |
author |
Silva, Larissa Gomes da |
author_facet |
Silva, Larissa Gomes da |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0110287926259408 |
dc.contributor.author.fl_str_mv |
Silva, Larissa Gomes da |
dc.contributor.advisor-co1.fl_str_mv |
Mizubuti, Eduardo Seiti Gomide |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8 |
dc.contributor.advisor-co2.fl_str_mv |
Barreto, Robert Weingart |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783300H6 |
dc.contributor.advisor1.fl_str_mv |
Pereira, Olinto Liparini |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4 |
dc.contributor.referee1.fl_str_mv |
Evans, Harold Charles |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4501250T6 |
dc.contributor.referee2.fl_str_mv |
Paula Júnior, Trazilbo José de |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7899276097018876 |
dc.contributor.referee3.fl_str_mv |
Nascimento, Robson José do |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/5574422695199565 |
contributor_str_mv |
Mizubuti, Eduardo Seiti Gomide Barreto, Robert Weingart Pereira, Olinto Liparini Evans, Harold Charles Paula Júnior, Trazilbo José de Nascimento, Robson José do |
dc.subject.por.fl_str_mv |
Mycosphaerellaceae Fungos fitopatogênicos Filogenia Micologia Mamão |
topic |
Mycosphaerellaceae Fungos fitopatogênicos Filogenia Micologia Mamão Mycosphaerellaceae Phytopathogenic fungi Phylogeny Mycology Papaya CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
dc.subject.eng.fl_str_mv |
Mycosphaerellaceae Phytopathogenic fungi Phylogeny Mycology Papaya |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
description |
Nowadays, Brazil is the largest producer of papaya and the third largest exporter of fruit in the world. Papaya can be affected by various diseases that prevent them from being marketed and, consequently, the exportation of the fruit. Among the fungal diseases, smallpox, or blight, caused by Asperisporium caricae, is one of the diseases that affect the orchards in all regions. This disease interferes directly in the profitability and viability of the production. Beyond its incidence on leaves, it can also cause lesions on the fruit surface, making them unsuitable for marketing. It s almost impossible to find publications related to the biology of the fungus and to the phylogenetic position of the species. A. caricae is considered by many authors as a biotrophic pathogen, based on the difficulty of cultivation and sporulation in vitro. Some aspects of the taxonomic genus Asperisporium are controversial, such as proving Mycosphaerella as a teleomorph organism, the phylogenetic relationships with other genera of the complex cercosporoidal and the separation of the genus Asperisporium from Passalora. Other authors relate M. caricae to the anamorph Phoma caricae-papaya (= Ascochyta caricapapaya), causal agent of stalk rot. However, through the manual identification the genera Phoma and Ascochyta are related to the teleomorph Didymella. The differences between the genders Didymella and Mycosphaerella is the presence of pseudo paraphyses in the last one. This feature is difficult to visualize in histological sections under a light microscope, thus being liable to misunderstand at the time of identification. The aims of the study were the isolation of A. caricae in pure culture, examining the growth and sporulation of the isolates in seven different media and make a morphological characterization of isolates. Beyond these, other aim was to verify the relationship between Asperisporium caricae and Mycosphaerella caricae by comparing nucleotides of three DNA regions (ITS rDNA, LSU and mtSSU) and the phylogenetic relationships with other genera of the complex cercosporoidal, by comparing the sequences obtained to sequences published in databases. It was possible to cultivate A. caricae in all tested media. The culture medium potato dextrose agar, supplemented or not with amino acids, were those who presented the best source to the fungus. Moreover, the species produced spores in vitro in all tested media, except in the medium consisting of papaya leaves. Through the molecular data and clusters obtained, it can be concluded that A. caricae belongs to the family Mycosphaerellaceae grouping it with species of true Passalora and Mycosphaerella. M. caricae was grouped with species of the genus Phoma, Ascochyta and Didymella, representatives of the order Pleosporales. Thus, it is possible that M. caricae is a species of Didymella and teleomorph of Phoma carica-papaya. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-23 |
dc.date.available.fl_str_mv |
2012-03-16 2015-03-26T13:37:45Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:37:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Larissa Gomes da. Isolation and growth of Asperisporium caricae and their phylogenetic relationship with Mycosphaerellaceae. 2010. 69 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/4395 |
identifier_str_mv |
SILVA, Larissa Gomes da. Isolation and growth of Asperisporium caricae and their phylogenetic relationship with Mycosphaerellaceae. 2010. 69 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010. |
url |
http://locus.ufv.br/handle/123456789/4395 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Mestrado em Fitopatologia |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Etiologia; Epidemiologia; Controle |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/4395/1/texto%20completo.pdf https://locus.ufv.br//bitstream/123456789/4395/2/texto%20completo.pdf.txt https://locus.ufv.br//bitstream/123456789/4395/3/texto%20completo.pdf.jpg |
bitstream.checksum.fl_str_mv |
02f10a0cc1d0bf768aa16e7168f46ef9 20144bf0fc576ce9e89d0e2580567743 2984204d459f4ceb642d1aa3cb990106 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801212866702868480 |