Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite

Detalhes bibliográficos
Autor(a) principal: Pellizzoni, Samantha Gusmão
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5054
Resumo: The objective was to estimate the urinary excretion of purine from the TDN consumption and determine the endogenous contribution in dairy cows. The experiment was conducted at the Unidade de Ensino, Pesquisa e Extensão em Gado de Leite (UEPE-GL) during the month of March 2010. Twelve Holstein cows purebred and crossbred, allocated in a completely randomized design with three treatments based on production of milk, which were assessed as high, medium and low bands by taking the following production: 33,50; 18,42 and 11.07 kg of milk / day, respectively.The diet consisted of corn silage ad libitum and concentrate fed at a rate of one kilogram for every three pounds of milk produced. The experimental period lasted 15 days, se ven to eight days for adaptation and implementation of collection of urine, faeces, blood and milk, as well as to evaluate the consumption and digestibility. Milkings were carried out in two hours, from 6:00 h and 16:00 h. The results were evaluated by analysis of variance, using the 5% level of probability of type I error, through the SAS, by using the t test to compare means. Relations between the daily excretion of purine derivatives and the consumption of total digestible nutrients (TDNI), digestible organic matter intake (DOMI) and dry matter intake (DMI) were assessed using linear regression. No significant differences (P>0.05) for lactose and milk fat, however, the percentage of protein was higher for cows with low production (P<0.05). Intakes of all nutrients, except for neutral detergent fiber corrected for ash and protein (NDF) and neutral detergent fiber indigestible (NDFi) were higher for animals for higher production. The digestibility of dry matter (DM), organic matter (OM), crude protein, and non-fiber carbohydrates and contents of total digestible nutrients (TDN) did not differ between treatments (P> 0.05), whereas the digestibility of ether extract and NDF were influenced by the level of milk production, and lower for cows of high production. The levels of urea nitrogen in plasma and milk and excretion of nitrogenous (N) in urine were highly correlated and higher (P<0.05) for more productive animals, indicating that the concentration varied with the level of milk production. Urinary excretions of purine derivatives (PD) and urea, as well as the relations of allantoin: creatinine, uric acid, creatinine, purine derivatives, creatine and urea, creatinine, when compared between different times of collection and spot urine collection total urine for 24 hours, no significant difference (P>0.05). The synthesis of ruminal microbial crude protein and excretion of PD were associated with intake of TDN and digestible organic matter (DOM), being raised from 23.48 mmol of SD for each kg of TDN consumed, 25.50 mmol SD for each kg of digestible organic matter, revealing that both relations can be used to make these estimates. The gathering spot can be used to estimate the daily excretion of purine derivatives and urea in the urine of cows, regardless of the level of production. Both the use of TDN intake and consumption of MOD can be used in order to estimate the urinary purine derivatives. Whereas the excretion of PD in mmol/kgPC0,75 and dry matter intake expressed as g/kg PC0,75, obtained the value of the fraction of endogenous DP mmol/kgPC0.2370,75.
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spelling Pellizzoni, Samantha Gusmãohttp://lattes.cnpq.br/7612615639640619Valadares Filho, Sebastião de Camposhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787028J6Marcondes, Marcos Ináciohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4731725A6Valadares, Rilene Ferreira Dinizhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787025E4Pina, Douglas dos Santoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778249J82015-03-26T13:46:56Z2011-10-312015-03-26T13:46:56Z2011-03-11PELLIZZONI, Samantha Gusmão. Estimation of urinary excretion of derivatives of purine from the consummate of NDT and determination of the contribution in endogenous milk cows. 2011. 72 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2011.http://locus.ufv.br/handle/123456789/5054The objective was to estimate the urinary excretion of purine from the TDN consumption and determine the endogenous contribution in dairy cows. The experiment was conducted at the Unidade de Ensino, Pesquisa e Extensão em Gado de Leite (UEPE-GL) during the month of March 2010. Twelve Holstein cows purebred and crossbred, allocated in a completely randomized design with three treatments based on production of milk, which were assessed as high, medium and low bands by taking the following production: 33,50; 18,42 and 11.07 kg of milk / day, respectively.The diet consisted of corn silage ad libitum and concentrate fed at a rate of one kilogram for every three pounds of milk produced. The experimental period lasted 15 days, se ven to eight days for adaptation and implementation of collection of urine, faeces, blood and milk, as well as to evaluate the consumption and digestibility. Milkings were carried out in two hours, from 6:00 h and 16:00 h. The results were evaluated by analysis of variance, using the 5% level of probability of type I error, through the SAS, by using the t test to compare means. Relations between the daily excretion of purine derivatives and the consumption of total digestible nutrients (TDNI), digestible organic matter intake (DOMI) and dry matter intake (DMI) were assessed using linear regression. No significant differences (P>0.05) for lactose and milk fat, however, the percentage of protein was higher for cows with low production (P<0.05). Intakes of all nutrients, except for neutral detergent fiber corrected for ash and protein (NDF) and neutral detergent fiber indigestible (NDFi) were higher for animals for higher production. The digestibility of dry matter (DM), organic matter (OM), crude protein, and non-fiber carbohydrates and contents of total digestible nutrients (TDN) did not differ between treatments (P> 0.05), whereas the digestibility of ether extract and NDF were influenced by the level of milk production, and lower for cows of high production. The levels of urea nitrogen in plasma and milk and excretion of nitrogenous (N) in urine were highly correlated and higher (P<0.05) for more productive animals, indicating that the concentration varied with the level of milk production. Urinary excretions of purine derivatives (PD) and urea, as well as the relations of allantoin: creatinine, uric acid, creatinine, purine derivatives, creatine and urea, creatinine, when compared between different times of collection and spot urine collection total urine for 24 hours, no significant difference (P>0.05). The synthesis of ruminal microbial crude protein and excretion of PD were associated with intake of TDN and digestible organic matter (DOM), being raised from 23.48 mmol of SD for each kg of TDN consumed, 25.50 mmol SD for each kg of digestible organic matter, revealing that both relations can be used to make these estimates. The gathering spot can be used to estimate the daily excretion of purine derivatives and urea in the urine of cows, regardless of the level of production. Both the use of TDN intake and consumption of MOD can be used in order to estimate the urinary purine derivatives. Whereas the excretion of PD in mmol/kgPC0,75 and dry matter intake expressed as g/kg PC0,75, obtained the value of the fraction of endogenous DP mmol/kgPC0.2370,75.Objetivou-se estimar a excreção urinária de derivados de purinas a partir do consumo NDT e determinar a contribuição endógena em vacas de leite. O experimento foi realizado na unidade de ensino, pesquisa e extensão em gado de leite (UEPE-GL) durante o mês de março de 2010. Utilizaram-se doze vacas da raça Holandesa, puras e mestiças, alocadas em delineamento inteiramente casualizado, com três tratamentos em função da produção de leite, que foram determinadas como Alta, Média e Baixa seguindo as seguintes faixas de produção: 33,50, 18,42 e 11,07 kg de leite/dia, respectivamente. A dieta foi constituída de silagem de milho à vontade e concentrado fornecido na proporção de um kg para cada três kg de leite produzido. O período experimental teve duração de 15 dias, sendo sete para adaptação e oito dias para realização das coletas de urina, fezes, sangue e leite, bem como para avaliação do consumo e digestibilidade aparente. As ordenhas foram realizadas em dois horários, às 6:00 h e 16:00 h. Os resultados foram avaliados por meio de análises de variância, utilizando-se nível de 5% de probabilidade para o erro tipo I, por intermédio do programa SAS, sendo utilizado o teste de T para comparação de médias. As relações entre as excreções diárias dos derivados de purinas e os consumos de nutrientes digestiveis totais (CNDT), de matéria orgânica digestível (CMOD) e de matéria seca (CMS) foram avaliadas através do modelo de regressão linear simples. Não houve diferença (P>0,05) para os teores de lactose e gordura do leite, porém, o percentual de proteína foi superior para vacas de baixa produção (P<0,05). Os consumos de todos os nutrientes, exceto o de fibra insolúvel em detergente neutro corrigida para cinzas e proteína (FDNcp) e fibra insolúvel em detergente neutro indigestível (FDNi), foram maiores para os animais de maior produção. As digestibilidades da matéria seca (MS), matéria orgânica (MO), proteína bruta, e carboidratos não fibrosos e os teores de nutrientes digestíveis totais (NDT) não diferiram entre os tratamentos (P>0,05), enquanto que as digestibilidades do extrato etéreo e da FDNcp, foram influenciadas pelo nível de produção de leite, sendo menores para vacas de Alta produção. Os teores de nitrogênio uréico no plasma e no leite e a excreção de compostos nitrogenados (N) na urina foram altamente correlacionados e superiores (P<0,05) para animais mais produtivos, indicando que a concentração variou com o nível de produção de leite. As excreções urinárias dos derivados de purinas (DP) e uréia, bem como as relações de alantoina:creatinina, ácido úrico:creatinina, derivados de purina:creatina e uréia:creatinina, quando comparadas entre os diferentes tempos de coleta spot de urina e coleta total de urina durante 24 horas, não apresentaram diferença significativa (P>0,05). A síntese ruminal de proteína bruta microbiana e a excreção de DP foram relacionadas com o consumo de NDT e de matéria orgânica digestível (MOD), sendo obtidos 23,48 mmol de DP para cada kg de NDT consumido e 25,50 mmol de DP para cada kg de MO digestível, sendo possível observar que ambas as relações podem ser utilizadas para realizar estas estimativas. A coleta spot pode ser utilizada para estimar a excreção diária de derivados de purinas e de uréia na urina em vacas, independentemente do nível de produção. Tanto a utilização do consumo de NDT quanto de MOD podem ser utilizados com o intuito de estimar os derivados de purinas urinários. Considerando a excreção de DP em mmol/kgPC0,75 e o consumo de matéria seca expresso em g/kg PC0,75, obteve-se o valor da fração endógena de DP de 0,237 mmol/kgPC0,75.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em Medicina VeterináriaUFVBRBiotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. deHolandesaLeiteNitrogênio microbianoHolstein cowsMilkMicrobial nitrogenCNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMALEstimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leiteEstimation of urinary excretion of derivatives of purine from the consummate of NDT and determination of the contribution in endogenous milk cowsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf308082https://locus.ufv.br//bitstream/123456789/5054/1/texto%20completo.pdfe30aa6dee1e9497438ca76f699ba5715MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain108471https://locus.ufv.br//bitstream/123456789/5054/2/texto%20completo.pdf.txtb4ec18f5a9862371e482f8045ee6c305MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3636https://locus.ufv.br//bitstream/123456789/5054/3/texto%20completo.pdf.jpgdee6bc494ea6adc66cbdade1595f7a3aMD53123456789/50542016-04-11 23:08:36.779oai:locus.ufv.br:123456789/5054Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-12T02:08:36LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite
dc.title.alternative.eng.fl_str_mv Estimation of urinary excretion of derivatives of purine from the consummate of NDT and determination of the contribution in endogenous milk cows
title Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite
spellingShingle Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite
Pellizzoni, Samantha Gusmão
Holandesa
Leite
Nitrogênio microbiano
Holstein cows
Milk
Microbial nitrogen
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL
title_short Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite
title_full Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite
title_fullStr Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite
title_full_unstemmed Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite
title_sort Estimativa da excreção urinária de derivados de purinas a partir do consumo de NDT e determinação da contribuição endógena em vacas de leite
author Pellizzoni, Samantha Gusmão
author_facet Pellizzoni, Samantha Gusmão
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/7612615639640619
dc.contributor.author.fl_str_mv Pellizzoni, Samantha Gusmão
dc.contributor.advisor-co1.fl_str_mv Valadares Filho, Sebastião de Campos
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787028J6
dc.contributor.advisor-co2.fl_str_mv Marcondes, Marcos Inácio
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4731725A6
dc.contributor.advisor1.fl_str_mv Valadares, Rilene Ferreira Diniz
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787025E4
dc.contributor.referee1.fl_str_mv Pina, Douglas dos Santos
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778249J8
contributor_str_mv Valadares Filho, Sebastião de Campos
Marcondes, Marcos Inácio
Valadares, Rilene Ferreira Diniz
Pina, Douglas dos Santos
dc.subject.por.fl_str_mv Holandesa
Leite
Nitrogênio microbiano
topic Holandesa
Leite
Nitrogênio microbiano
Holstein cows
Milk
Microbial nitrogen
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL
dc.subject.eng.fl_str_mv Holstein cows
Milk
Microbial nitrogen
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL
description The objective was to estimate the urinary excretion of purine from the TDN consumption and determine the endogenous contribution in dairy cows. The experiment was conducted at the Unidade de Ensino, Pesquisa e Extensão em Gado de Leite (UEPE-GL) during the month of March 2010. Twelve Holstein cows purebred and crossbred, allocated in a completely randomized design with three treatments based on production of milk, which were assessed as high, medium and low bands by taking the following production: 33,50; 18,42 and 11.07 kg of milk / day, respectively.The diet consisted of corn silage ad libitum and concentrate fed at a rate of one kilogram for every three pounds of milk produced. The experimental period lasted 15 days, se ven to eight days for adaptation and implementation of collection of urine, faeces, blood and milk, as well as to evaluate the consumption and digestibility. Milkings were carried out in two hours, from 6:00 h and 16:00 h. The results were evaluated by analysis of variance, using the 5% level of probability of type I error, through the SAS, by using the t test to compare means. Relations between the daily excretion of purine derivatives and the consumption of total digestible nutrients (TDNI), digestible organic matter intake (DOMI) and dry matter intake (DMI) were assessed using linear regression. No significant differences (P>0.05) for lactose and milk fat, however, the percentage of protein was higher for cows with low production (P<0.05). Intakes of all nutrients, except for neutral detergent fiber corrected for ash and protein (NDF) and neutral detergent fiber indigestible (NDFi) were higher for animals for higher production. The digestibility of dry matter (DM), organic matter (OM), crude protein, and non-fiber carbohydrates and contents of total digestible nutrients (TDN) did not differ between treatments (P> 0.05), whereas the digestibility of ether extract and NDF were influenced by the level of milk production, and lower for cows of high production. The levels of urea nitrogen in plasma and milk and excretion of nitrogenous (N) in urine were highly correlated and higher (P<0.05) for more productive animals, indicating that the concentration varied with the level of milk production. Urinary excretions of purine derivatives (PD) and urea, as well as the relations of allantoin: creatinine, uric acid, creatinine, purine derivatives, creatine and urea, creatinine, when compared between different times of collection and spot urine collection total urine for 24 hours, no significant difference (P>0.05). The synthesis of ruminal microbial crude protein and excretion of PD were associated with intake of TDN and digestible organic matter (DOM), being raised from 23.48 mmol of SD for each kg of TDN consumed, 25.50 mmol SD for each kg of digestible organic matter, revealing that both relations can be used to make these estimates. The gathering spot can be used to estimate the daily excretion of purine derivatives and urea in the urine of cows, regardless of the level of production. Both the use of TDN intake and consumption of MOD can be used in order to estimate the urinary purine derivatives. Whereas the excretion of PD in mmol/kgPC0,75 and dry matter intake expressed as g/kg PC0,75, obtained the value of the fraction of endogenous DP mmol/kgPC0.2370,75.
publishDate 2011
dc.date.available.fl_str_mv 2011-10-31
2015-03-26T13:46:56Z
dc.date.issued.fl_str_mv 2011-03-11
dc.date.accessioned.fl_str_mv 2015-03-26T13:46:56Z
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dc.identifier.citation.fl_str_mv PELLIZZONI, Samantha Gusmão. Estimation of urinary excretion of derivatives of purine from the consummate of NDT and determination of the contribution in endogenous milk cows. 2011. 72 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2011.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5054
identifier_str_mv PELLIZZONI, Samantha Gusmão. Estimation of urinary excretion of derivatives of purine from the consummate of NDT and determination of the contribution in endogenous milk cows. 2011. 72 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2011.
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