Development of microsatellite markers for the guava rust fungus, Puccinia psidii

Detalhes bibliográficos
Autor(a) principal: Zhong, Shaobin
Data de Publicação: 2008
Outros Autores: Yang, Baojun, Alfenas, Acelino Couto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1111/j.1471-8286.2007.01952.x
http://www.locus.ufv.br/handle/123456789/11888
Resumo: We developed and characterized 15 polymorphic microsatellite markers present in the genome of the guava rust fungus, Puccinia psidii. The primers for these microsatellite markers were designed by sequencing clones from a genomic DNA library enriched for a simple sequence repeat (SSR) motif of (AG). All these 15 primer pairs successfully amplified DNA fragments from a sample of 22 P. psidii isolates, revealing a total of 71 alleles. The observed heterozygosity at the 15 loci ranged from 0.05 to 1.00. The SSR markers developed would be useful for population genetics study of the rust fungus.
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spelling Zhong, ShaobinYang, BaojunAlfenas, Acelino Couto2017-10-05T12:24:20Z2017-10-05T12:24:20Z2008-031755-0998https://doi.org/10.1111/j.1471-8286.2007.01952.xhttp://www.locus.ufv.br/handle/123456789/11888We developed and characterized 15 polymorphic microsatellite markers present in the genome of the guava rust fungus, Puccinia psidii. The primers for these microsatellite markers were designed by sequencing clones from a genomic DNA library enriched for a simple sequence repeat (SSR) motif of (AG). All these 15 primer pairs successfully amplified DNA fragments from a sample of 22 P. psidii isolates, revealing a total of 71 alleles. The observed heterozygosity at the 15 loci ranged from 0.05 to 1.00. The SSR markers developed would be useful for population genetics study of the rust fungus.engMolecular Ecology ResourcesVolume 8, Issue 2 Pages 348–350Creative Commons Attributioninfo:eu-repo/semantics/openAccessBasidiomycetesGuava rustMicrosatelliteMyrtaceaePuccinia psidiiSimple sequence repeatDevelopment of microsatellite markers for the guava rust fungus, Puccinia psidiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALZHONG_et_al-2008-Molecular_Ecology_Resources.pdfZHONG_et_al-2008-Molecular_Ecology_Resources.pdftexto completoapplication/pdf62588https://locus.ufv.br//bitstream/123456789/11888/1/ZHONG_et_al-2008-Molecular_Ecology_Resources.pdfb4981901efac49f8d7b502f906fb02cdMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/11888/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILZHONG_et_al-2008-Molecular_Ecology_Resources.pdf.jpgZHONG_et_al-2008-Molecular_Ecology_Resources.pdf.jpgIM Thumbnailimage/jpeg5066https://locus.ufv.br//bitstream/123456789/11888/3/ZHONG_et_al-2008-Molecular_Ecology_Resources.pdf.jpgd405c3d6f5b3bc0739ec0bbaa979774bMD53123456789/118882017-10-05 23:00:29.955oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-06T02:00:29LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Development of microsatellite markers for the guava rust fungus, Puccinia psidii
title Development of microsatellite markers for the guava rust fungus, Puccinia psidii
spellingShingle Development of microsatellite markers for the guava rust fungus, Puccinia psidii
Zhong, Shaobin
Basidiomycetes
Guava rust
Microsatellite
Myrtaceae
Puccinia psidii
Simple sequence repeat
title_short Development of microsatellite markers for the guava rust fungus, Puccinia psidii
title_full Development of microsatellite markers for the guava rust fungus, Puccinia psidii
title_fullStr Development of microsatellite markers for the guava rust fungus, Puccinia psidii
title_full_unstemmed Development of microsatellite markers for the guava rust fungus, Puccinia psidii
title_sort Development of microsatellite markers for the guava rust fungus, Puccinia psidii
author Zhong, Shaobin
author_facet Zhong, Shaobin
Yang, Baojun
Alfenas, Acelino Couto
author_role author
author2 Yang, Baojun
Alfenas, Acelino Couto
author2_role author
author
dc.contributor.author.fl_str_mv Zhong, Shaobin
Yang, Baojun
Alfenas, Acelino Couto
dc.subject.pt-BR.fl_str_mv Basidiomycetes
Guava rust
Microsatellite
Myrtaceae
Puccinia psidii
Simple sequence repeat
topic Basidiomycetes
Guava rust
Microsatellite
Myrtaceae
Puccinia psidii
Simple sequence repeat
description We developed and characterized 15 polymorphic microsatellite markers present in the genome of the guava rust fungus, Puccinia psidii. The primers for these microsatellite markers were designed by sequencing clones from a genomic DNA library enriched for a simple sequence repeat (SSR) motif of (AG). All these 15 primer pairs successfully amplified DNA fragments from a sample of 22 P. psidii isolates, revealing a total of 71 alleles. The observed heterozygosity at the 15 loci ranged from 0.05 to 1.00. The SSR markers developed would be useful for population genetics study of the rust fungus.
publishDate 2008
dc.date.issued.fl_str_mv 2008-03
dc.date.accessioned.fl_str_mv 2017-10-05T12:24:20Z
dc.date.available.fl_str_mv 2017-10-05T12:24:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1111/j.1471-8286.2007.01952.x
http://www.locus.ufv.br/handle/123456789/11888
dc.identifier.issn.none.fl_str_mv 1755-0998
identifier_str_mv 1755-0998
url https://doi.org/10.1111/j.1471-8286.2007.01952.x
http://www.locus.ufv.br/handle/123456789/11888
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 8, Issue 2 Pages 348–350
dc.rights.driver.fl_str_mv Creative Commons Attribution
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Creative Commons Attribution
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Molecular Ecology Resources
publisher.none.fl_str_mv Molecular Ecology Resources
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
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