Electrophoretic characterization ofaspergillus nidulans strains with chromosomal duplications

• Autor(a) principal: Queiroz, Marisa V. de
• Data de Publicação: 2000
• Outros Autores: Pizzirani-Kleiner, Aline Aparecida, Azevedo, João Lúcio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://dx.doi.org/10.1590/S1415-47572000000200009; http://locus.ufv.br//handle/123456789/26018
• topic: Electrophoretic; Strains; Chromosomal; Aspergillus nidulans

Pulsed-field gel electrophoresis was used to characterize strains ofAspergillus nidulans with a chromosomal duplication Dp(I-II). Morphologically deteriorated and improved variants of these strains were also analyzed. The electrophoretic karyotype demonstrated that in two duplicated strains (A and B) the 4.2 Mb band, which corresponds to chromosome II, was absent and a new band was observed. Hybridization studies using the uapA (chromosome I) and wA (chromosome II) genes demonstrated that the new band corresponded to chromosome II plus the duplicated segment of chromosome I. The size of the chromosomal duplication was approximately 1.0 Mb. Analysis of the chromosomal bands of a morphologically improved strain showed that the duplicated segment of chromosome I was completely lost. The morphologically deteriorated variants V9 and V17 had the same karyotype as the duplicated strains. However, the deteriorated variant V5 lost part of chromosome I and had a rearrangement involving chromosome V. This rearrangement may have resulted from the mutagenic treatment used to obtain the genetic markers. Pulsed-field gel electrophoresis was found to be an excellent tool for locating chromosomal rearrangements.