Data analysis for 16S microbial profiling from different benchtop sequencing platforms

Detalhes bibliográficos
Autor(a) principal: Pylro, Victor S.
Data de Publicação: 2014
Outros Autores: Roesch, Luiz Fernando W., Morais, Daniel K., Clark, Ian M., Hirsch, Penny R., Tótola, Marcos R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.mimet.2014.08.018
http://www.locus.ufv.br/handle/123456789/18661
Resumo: Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied.
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spelling Pylro, Victor S.Roesch, Luiz Fernando W.Morais, Daniel K.Clark, Ian M.Hirsch, Penny R.Tótola, Marcos R.2018-04-06T09:51:17Z2018-04-06T09:51:17Z2014-08-270167-7012https://doi.org/10.1016/j.mimet.2014.08.018http://www.locus.ufv.br/handle/123456789/18661Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied.engJournal of Microbiological MethodsVolume 107, Pages 30-37, December 2014Next generation sequencingMicrobial community analysisAmpliconsAlpha diversityBeta diversityData analysis for 16S microbial profiling from different benchtop sequencing platformsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf707244https://locus.ufv.br//bitstream/123456789/18661/5/artigo.pdf0298db70d732c3ec0d7f8e56dd74c133MD55LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18661/6/license.txt8a4605be74aa9ea9d79846c1fba20a33MD56THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg6044https://locus.ufv.br//bitstream/123456789/18661/7/artigo.pdf.jpgb2c8e291650ff8e584f771b83c8d5b76MD57123456789/186612018-04-06 23:00:32.964oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-07T02:00:32LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Data analysis for 16S microbial profiling from different benchtop sequencing platforms
title Data analysis for 16S microbial profiling from different benchtop sequencing platforms
spellingShingle Data analysis for 16S microbial profiling from different benchtop sequencing platforms
Pylro, Victor S.
Next generation sequencing
Microbial community analysis
Amplicons
Alpha diversity
Beta diversity
title_short Data analysis for 16S microbial profiling from different benchtop sequencing platforms
title_full Data analysis for 16S microbial profiling from different benchtop sequencing platforms
title_fullStr Data analysis for 16S microbial profiling from different benchtop sequencing platforms
title_full_unstemmed Data analysis for 16S microbial profiling from different benchtop sequencing platforms
title_sort Data analysis for 16S microbial profiling from different benchtop sequencing platforms
author Pylro, Victor S.
author_facet Pylro, Victor S.
Roesch, Luiz Fernando W.
Morais, Daniel K.
Clark, Ian M.
Hirsch, Penny R.
Tótola, Marcos R.
author_role author
author2 Roesch, Luiz Fernando W.
Morais, Daniel K.
Clark, Ian M.
Hirsch, Penny R.
Tótola, Marcos R.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pylro, Victor S.
Roesch, Luiz Fernando W.
Morais, Daniel K.
Clark, Ian M.
Hirsch, Penny R.
Tótola, Marcos R.
dc.subject.pt-BR.fl_str_mv Next generation sequencing
Microbial community analysis
Amplicons
Alpha diversity
Beta diversity
topic Next generation sequencing
Microbial community analysis
Amplicons
Alpha diversity
Beta diversity
description Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied.
publishDate 2014
dc.date.issued.fl_str_mv 2014-08-27
dc.date.accessioned.fl_str_mv 2018-04-06T09:51:17Z
dc.date.available.fl_str_mv 2018-04-06T09:51:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.mimet.2014.08.018
http://www.locus.ufv.br/handle/123456789/18661
dc.identifier.issn.none.fl_str_mv 0167-7012
identifier_str_mv 0167-7012
url https://doi.org/10.1016/j.mimet.2014.08.018
http://www.locus.ufv.br/handle/123456789/18661
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 107, Pages 30-37, December 2014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Journal of Microbiological Methods
publisher.none.fl_str_mv Journal of Microbiological Methods
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
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reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
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