Differentially expressed proteins during an incompatible interaction between common bean and the fungus Pseudocercospora griseola

• Autor(a) principal: Borges, Leandro Luiz
• Data de Publicação: 2013
• Outros Autores: Santana, Fernanda Abreu, Castro, Isabel Samila Lima, Arruda, Klever Márcio Antunes, Ramos, Humberto Josué de Oliveira, Moreira, Maurilio Alves, Barros, Everaldo Gonçalves de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1007/s11032-013-9922-0; http://www.locus.ufv.br/handle/123456789/21330
• topic: Phaseolus vulgaris; Angular leaf spot; Proteome; Mass spectrometry

The common bean (Phaseolus vulgaris L.) is the main source of protein and an important source of minerals in several countries around the world. Angular leaf spot, caused by the fungus Pseudocercospora griseola, is one of the major diseases of the common bean. In this work, we used two-dimensional gel electrophoresis and mass spectrometry to analyze alterations in the proteome of common bean leaves challenged with an incompatible race of P. griseola. Twenty-three differentially expressed proteins were detected in leaves of cultivar AND 277 collected at 12, 24 and 48 h after inoculation. The proteins were digested with trypsin and submitted to MALDI-TOF/TOF and MicrOTOF-Q electrospray mass spectrometry. Nineteen of them were identified upon MS/MS fragmentation. Most of these proteins are involved with amino acid metabolism, terpenoid metabolism, phenylpropanoid biosynthesis, antioxidant systems, vitamin and cofactor metabolism, plant–pathogen interaction, carbohydrate metabolism, photosynthesis, or genetic information processing, showing that the interaction in this pathosystem affects different genes from various metabolic pathways and processes.