In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation

Detalhes bibliográficos
Autor(a) principal: Camargo, Anderson Carlos
Data de Publicação: 2015
Outros Autores: Paula, Otávio Almeida Lino de, Todorov, Svetoslav Dimitrov, Nero, Luís Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1007/s12010-015-1941-3
http://www.locus.ufv.br/handle/123456789/18944
Resumo: The present study aimed to assess the activity of cell-free supernatant (CFS) containing bacteriocins on the formation and maintenance of biofilms developed by Listeria monocytogenes, and the associated effect of bacteriocins and ethylene-diamine-tetra-acetic acid (EDTA) on the formed biofilm. CFS from 9 lactic acid bacteria (LAB) strains was tested for inhibitory activity against 85 L. monocytogenes isolates and 21 LAB strains. Then, 12 L. monocytogenes strains were selected based on genetic profiles and sensitivity to CFS and were subjected to an in vitro assay to assess biofilm formation in microtiter plates, considering different culture media and incubation conditions. Based on these results, 6 L. monocytogenes strains were subjected to the same in vitro procedure to assess biofilm formation, being co-inoculated with CFS. In addition, these strains were subjected to the same in vitro procedure, modified by adding the CFS after biofilm formation. Relevant decrease in biofilm formation was observed in the first experiment, but CFS added after biofilm formation did not eliminate them. CFS from Lactobacillus curvatus ET31 were selected due to its anti-biofilm activity, being associated to EDTA at different concentrations and tested for biofilm control of three strains of L. monocytogenes, using the same in vitro procedure described previously. Concentrated bacteriocin presented poor performance in eliminating formed biofilms, and EDTA concentration presented no evident interference on biofilm elimination. Twelve selected L. monocytogenes strains were positive for investigated virulence makers and negative for luxS gene, recognized as being involved in biofilm formation. Selected L. monocytogenes strains were able to produce biofilms under different conditions. CFSs have the potential to prevent biofilm formation, but they were not able to destroy already formed biofilms. Nevertheless, low concentrations of CFS combined with EDTA caused a relevant reduction in already formed biofilms, but this association was not able to eliminate them. The activity of selected CFS was demonstrated against L. monocytogenes-formed biofilms, being more effective when associated to EDTA at different concentrations.
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spelling In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formationListeria monocytogenesBiofilmBacteriocinLactic acid bacteriaThe present study aimed to assess the activity of cell-free supernatant (CFS) containing bacteriocins on the formation and maintenance of biofilms developed by Listeria monocytogenes, and the associated effect of bacteriocins and ethylene-diamine-tetra-acetic acid (EDTA) on the formed biofilm. CFS from 9 lactic acid bacteria (LAB) strains was tested for inhibitory activity against 85 L. monocytogenes isolates and 21 LAB strains. Then, 12 L. monocytogenes strains were selected based on genetic profiles and sensitivity to CFS and were subjected to an in vitro assay to assess biofilm formation in microtiter plates, considering different culture media and incubation conditions. Based on these results, 6 L. monocytogenes strains were subjected to the same in vitro procedure to assess biofilm formation, being co-inoculated with CFS. In addition, these strains were subjected to the same in vitro procedure, modified by adding the CFS after biofilm formation. Relevant decrease in biofilm formation was observed in the first experiment, but CFS added after biofilm formation did not eliminate them. CFS from Lactobacillus curvatus ET31 were selected due to its anti-biofilm activity, being associated to EDTA at different concentrations and tested for biofilm control of three strains of L. monocytogenes, using the same in vitro procedure described previously. Concentrated bacteriocin presented poor performance in eliminating formed biofilms, and EDTA concentration presented no evident interference on biofilm elimination. Twelve selected L. monocytogenes strains were positive for investigated virulence makers and negative for luxS gene, recognized as being involved in biofilm formation. Selected L. monocytogenes strains were able to produce biofilms under different conditions. CFSs have the potential to prevent biofilm formation, but they were not able to destroy already formed biofilms. Nevertheless, low concentrations of CFS combined with EDTA caused a relevant reduction in already formed biofilms, but this association was not able to eliminate them. The activity of selected CFS was demonstrated against L. monocytogenes-formed biofilms, being more effective when associated to EDTA at different concentrations.Applied Biochemistry and Biotechnology2018-04-20T14:23:20Z2018-04-20T14:23:20Z2015-12-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15590291https://doi.org/10.1007/s12010-015-1941-3http://www.locus.ufv.br/handle/123456789/18944engv. 178, Issue 6, p. 1239–1251, March 2016Springer Science+Business Media New Yorkinfo:eu-repo/semantics/openAccessCamargo, Anderson CarlosPaula, Otávio Almeida Lino deTodorov, Svetoslav DimitrovNero, Luís Augustoreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:52:31Zoai:locus.ufv.br:123456789/18944Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:52:31LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation
title In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation
spellingShingle In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation
Camargo, Anderson Carlos
Listeria monocytogenes
Biofilm
Bacteriocin
Lactic acid bacteria
title_short In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation
title_full In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation
title_fullStr In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation
title_full_unstemmed In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation
title_sort In Vitro evaluation of bacteriocins activity against Listeria monocytogenes biofilm formation
author Camargo, Anderson Carlos
author_facet Camargo, Anderson Carlos
Paula, Otávio Almeida Lino de
Todorov, Svetoslav Dimitrov
Nero, Luís Augusto
author_role author
author2 Paula, Otávio Almeida Lino de
Todorov, Svetoslav Dimitrov
Nero, Luís Augusto
author2_role author
author
author
dc.contributor.author.fl_str_mv Camargo, Anderson Carlos
Paula, Otávio Almeida Lino de
Todorov, Svetoslav Dimitrov
Nero, Luís Augusto
dc.subject.por.fl_str_mv Listeria monocytogenes
Biofilm
Bacteriocin
Lactic acid bacteria
topic Listeria monocytogenes
Biofilm
Bacteriocin
Lactic acid bacteria
description The present study aimed to assess the activity of cell-free supernatant (CFS) containing bacteriocins on the formation and maintenance of biofilms developed by Listeria monocytogenes, and the associated effect of bacteriocins and ethylene-diamine-tetra-acetic acid (EDTA) on the formed biofilm. CFS from 9 lactic acid bacteria (LAB) strains was tested for inhibitory activity against 85 L. monocytogenes isolates and 21 LAB strains. Then, 12 L. monocytogenes strains were selected based on genetic profiles and sensitivity to CFS and were subjected to an in vitro assay to assess biofilm formation in microtiter plates, considering different culture media and incubation conditions. Based on these results, 6 L. monocytogenes strains were subjected to the same in vitro procedure to assess biofilm formation, being co-inoculated with CFS. In addition, these strains were subjected to the same in vitro procedure, modified by adding the CFS after biofilm formation. Relevant decrease in biofilm formation was observed in the first experiment, but CFS added after biofilm formation did not eliminate them. CFS from Lactobacillus curvatus ET31 were selected due to its anti-biofilm activity, being associated to EDTA at different concentrations and tested for biofilm control of three strains of L. monocytogenes, using the same in vitro procedure described previously. Concentrated bacteriocin presented poor performance in eliminating formed biofilms, and EDTA concentration presented no evident interference on biofilm elimination. Twelve selected L. monocytogenes strains were positive for investigated virulence makers and negative for luxS gene, recognized as being involved in biofilm formation. Selected L. monocytogenes strains were able to produce biofilms under different conditions. CFSs have the potential to prevent biofilm formation, but they were not able to destroy already formed biofilms. Nevertheless, low concentrations of CFS combined with EDTA caused a relevant reduction in already formed biofilms, but this association was not able to eliminate them. The activity of selected CFS was demonstrated against L. monocytogenes-formed biofilms, being more effective when associated to EDTA at different concentrations.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-10
2018-04-20T14:23:20Z
2018-04-20T14:23:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 15590291
https://doi.org/10.1007/s12010-015-1941-3
http://www.locus.ufv.br/handle/123456789/18944
identifier_str_mv 15590291
url https://doi.org/10.1007/s12010-015-1941-3
http://www.locus.ufv.br/handle/123456789/18944
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 178, Issue 6, p. 1239–1251, March 2016
dc.rights.driver.fl_str_mv Springer Science+Business Media New York
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Springer Science+Business Media New York
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Applied Biochemistry and Biotechnology
publisher.none.fl_str_mv Applied Biochemistry and Biotechnology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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