Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro

Detalhes bibliográficos
Autor(a) principal: Krause-Sakate, Renate
Data de Publicação: 2007
Outros Autores: Richard-Forget, Florence, Redondo, Elise, Pavan, Marcelo Agenor, Zerbini, Francisco Murilo, Candresse, Thierry, Gall, Olivier Le
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S0100-54052007000200003
http://locus.ufv.br//handle/123456789/26199
Resumo: Dois isolados de Lettuce mosaic virus capazes de contornar a resistência conferida pelo gene mo12 em alface, LMV-AF199 proveniente do Brasil e LMV-E um isolado europeu, foram avaliados quanto à rapidez e à severidade dos sintomas induzidos em alface variedade Salinas 88 (mo12). Os sintomas de mosaico induzidos pelo isolado LMV-AF-199 em Salinas 88 são mais severos e aparecem aos 7 dias após a inoculação (dpi), enquanto que para o isolado LMV-E os sintomas são visíveis somente a partir dos 15 dpi. Com o intuito de identificar a região do genoma viral responsável por este fenótipo, vírus recombinantes foram construídos entre estes dois isolados, e o fenótipo avaliado quanto a rapidez e severidade dos sintomas em Salinas-88. A região codificadora para as proteínas P1 e Hc-Pro do LMV-AF199 foi associada com o aumento da virulência deste isolado em Salinas-88.
id UFV_6f45069baff23de0eee3f4ddefa6f1f7
oai_identifier_str oai:locus.ufv.br:123456789/26199
network_acronym_str UFV
network_name_str LOCUS Repositório Institucional da UFV
repository_id_str 2145
spelling Krause-Sakate, RenateRichard-Forget, FlorenceRedondo, ElisePavan, Marcelo AgenorZerbini, Francisco MuriloCandresse, ThierryGall, Olivier Le2019-07-11T14:13:12Z2019-07-11T14:13:12Z2007-041980-5454http://dx.doi.org/10.1590/S0100-54052007000200003http://locus.ufv.br//handle/123456789/26199Dois isolados de Lettuce mosaic virus capazes de contornar a resistência conferida pelo gene mo12 em alface, LMV-AF199 proveniente do Brasil e LMV-E um isolado europeu, foram avaliados quanto à rapidez e à severidade dos sintomas induzidos em alface variedade Salinas 88 (mo12). Os sintomas de mosaico induzidos pelo isolado LMV-AF-199 em Salinas 88 são mais severos e aparecem aos 7 dias após a inoculação (dpi), enquanto que para o isolado LMV-E os sintomas são visíveis somente a partir dos 15 dpi. Com o intuito de identificar a região do genoma viral responsável por este fenótipo, vírus recombinantes foram construídos entre estes dois isolados, e o fenótipo avaliado quanto a rapidez e severidade dos sintomas em Salinas-88. A região codificadora para as proteínas P1 e Hc-Pro do LMV-AF199 foi associada com o aumento da virulência deste isolado em Salinas-88.Two Lettuce mosaic virus isolates capable of overcoming the resistance afforded by the resistance gene mo12 in lettuce, LMV-AF199 from Brazil, and LMV-E, an European isolate, were evaluated for the rapidity and severity of symptoms induced on the lettuce variety Salinas 88 (mo12). The mosaic symptoms on Salinas 88 plants inoculated with LMV-AF199 appeared 7 days post-inoculation (dpi) and 15 dpi for LMV-E. The symptoms induced by LMV-AF199 in this cultivar were also more severe than those induced by LMV-E. In order to identify the region of the viral genome responsible for this phenotype, recombinant viruses were constructed between these isolates and the phenotype of each recombinant was analysed. The region encoding proteins P1 and HcPro from LMV-AF199 was associated with the increased virulence in Salinas 88.engSumma Phytopathologicav. 33, n. 2, p. 119- 123, abr./ jun. 2007Gene silencingLactuca sativaInfectious cDNAPotyvirusSilenciamento gênicocDNA infecciosoQuantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCProinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfartigoapplication/pdf288987https://locus.ufv.br//bitstream/123456789/26199/1/artigo.pdf78f1d5d2234099b967dd7dbe8492c4dcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/26199/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/261992019-07-11 11:42:37.578oai:locus.ufv.br:123456789/26199Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-07-11T14:42:37LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro
title Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro
spellingShingle Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro
Krause-Sakate, Renate
Gene silencing
Lactuca sativa
Infectious cDNA
Potyvirus
Silenciamento gênico
cDNA infeccioso
title_short Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro
title_full Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro
title_fullStr Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro
title_full_unstemmed Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro
title_sort Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro
author Krause-Sakate, Renate
author_facet Krause-Sakate, Renate
Richard-Forget, Florence
Redondo, Elise
Pavan, Marcelo Agenor
Zerbini, Francisco Murilo
Candresse, Thierry
Gall, Olivier Le
author_role author
author2 Richard-Forget, Florence
Redondo, Elise
Pavan, Marcelo Agenor
Zerbini, Francisco Murilo
Candresse, Thierry
Gall, Olivier Le
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Krause-Sakate, Renate
Richard-Forget, Florence
Redondo, Elise
Pavan, Marcelo Agenor
Zerbini, Francisco Murilo
Candresse, Thierry
Gall, Olivier Le
dc.subject.pt-BR.fl_str_mv Gene silencing
Lactuca sativa
Infectious cDNA
Potyvirus
Silenciamento gênico
cDNA infeccioso
topic Gene silencing
Lactuca sativa
Infectious cDNA
Potyvirus
Silenciamento gênico
cDNA infeccioso
description Dois isolados de Lettuce mosaic virus capazes de contornar a resistência conferida pelo gene mo12 em alface, LMV-AF199 proveniente do Brasil e LMV-E um isolado europeu, foram avaliados quanto à rapidez e à severidade dos sintomas induzidos em alface variedade Salinas 88 (mo12). Os sintomas de mosaico induzidos pelo isolado LMV-AF-199 em Salinas 88 são mais severos e aparecem aos 7 dias após a inoculação (dpi), enquanto que para o isolado LMV-E os sintomas são visíveis somente a partir dos 15 dpi. Com o intuito de identificar a região do genoma viral responsável por este fenótipo, vírus recombinantes foram construídos entre estes dois isolados, e o fenótipo avaliado quanto a rapidez e severidade dos sintomas em Salinas-88. A região codificadora para as proteínas P1 e Hc-Pro do LMV-AF199 foi associada com o aumento da virulência deste isolado em Salinas-88.
publishDate 2007
dc.date.issued.fl_str_mv 2007-04
dc.date.accessioned.fl_str_mv 2019-07-11T14:13:12Z
dc.date.available.fl_str_mv 2019-07-11T14:13:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-54052007000200003
http://locus.ufv.br//handle/123456789/26199
dc.identifier.issn.none.fl_str_mv 1980-5454
identifier_str_mv 1980-5454
url http://dx.doi.org/10.1590/S0100-54052007000200003
http://locus.ufv.br//handle/123456789/26199
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 33, n. 2, p. 119- 123, abr./ jun. 2007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Summa Phytopathologica
publisher.none.fl_str_mv Summa Phytopathologica
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/26199/1/artigo.pdf
https://locus.ufv.br//bitstream/123456789/26199/2/license.txt
bitstream.checksum.fl_str_mv 78f1d5d2234099b967dd7dbe8492c4dc
8a4605be74aa9ea9d79846c1fba20a33
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
_version_ 1801213072520511488

Registros relacionados